LOCUS       sequence-256439-        9672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human C1ORF43 with C-terminal GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, 
            Jimenez-Morales D, Stevenson E, Krogan NJ, Swaney DL, Snyder MP, 
            Mukherjee S, Bassik MC
  TITLE     Systematic Identification of Host Cell Regulators of Legionella 
            pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
  JOURNAL   Cell Host Microbe. 2019 Oct 9;26(4):551-563.e6. doi: 
            10.1016/j.chom.2019.08.017. Epub 2019 Sep 17.
  PUBMED    31540829
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.08.017"; journalName: "Cell Host Microbe"; 
            date: "2019-10-9- 9"; volume: "26"; issue: "4"; pages: "551-563.e6"
COMMENT     Sequence Label: C1ORF43-GFP
FEATURES             Location/Qualifiers
     source          1..9672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..282
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          283..1221
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1178..1198
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1242..1838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1242..1261)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1738..1758
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1848..1901
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1908..1917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2685..2687,2688..2690,2691..3404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2730..2751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2991..3010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3338..3359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3427..3460)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3516..4104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3569..3589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3987..3998)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4107..4123)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4108..4124)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4633..4813
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4875..5463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4955..4974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5634..6425,6426..6494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6257..6276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6495..6599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6674..6693)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6865..7244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7246..7444
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7399..7419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7462..7642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7689..7814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8311..8544
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8729..8773
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9071..9188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(9441..9474)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9478..9498
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg ccatgcatgt
       61 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      121 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      181 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      241 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      301 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      361 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      421 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      481 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      541 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      601 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      661 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      721 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
      781 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
      841 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
      901 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
      961 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1021 cgcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1081 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1141 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1201 agtttttttc ttccatttca ggtgtcgtga gctagcccac catgaccgag tacaagccca
     1261 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     1321 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     1381 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     1441 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     1501 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     1561 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     1621 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     1681 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     1741 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     1801 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atcgggagag ggcagaggaa
     1861 gtctgctaac atgcggtgac gtcgaggaga atcctggccc accggtcgcc accatggcgt
     1921 ccggcagtaa ctggctctcc ggggtgaatg tcgtgctggt gatggcctac gggagcctgg
     1981 tgtttgtact gctatttatt tttgtgaaga ggcaaatcat gcgctttgca atgaaatctc
     2041 gaaggggacc tcatgtccct gtgggacaca atgcccccaa ggacttgaaa gaggagattg
     2101 atattcgact ctccagggtt caggatatca agtatgagcc ccagctcctt gcagatgatg
     2161 atgctagact actacaactg gaaacccagg gaaatcaaag ttgctacaac tatctgtata
     2221 ggatgaaagc tctggatgcc attcgtacct ctgagatccc atttcattct gaaggccggc
     2281 atccccgttc cttaatgggc aagaatttcc gctcctacct gctggatctg cgaaacacta
     2341 gtacgccttt caagggtgta cgcaaagcac tcattgatac ccttttggat ggctatgaaa
     2401 cagcccgcta tgggacaggg gtctttggcc agaatgagta cctacgctat caggaggccc
     2461 tgagtgagct ggccactgcg gttaaagcac gaattgggag ctctcagcga catcaccagt
     2521 cagcagccaa agacctaact cagtcccctg aggtctcccc aacaaccatc caggtgacat
     2581 acctcccctc cagtcagaag agtaaacgtg ccaagcactt ccttgaattg aagagcttta
     2641 aggataacta taacacattg gagagtactc tgggtggaag tggtatggtg agcaagggcg
     2701 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2761 acaagttcag cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2821 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2881 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2941 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3001 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3061 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3121 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact
     3181 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     3241 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     3301 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3361 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataagaa ttcgtcgagg
     3421 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
     3481 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     3541 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3601 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3661 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3721 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3781 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3841 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3901 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3961 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4021 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4081 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     4141 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4201 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     4261 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4321 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     4381 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     4441 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     4501 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     4561 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     4621 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4801 aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6601 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     6661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     6721 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     6781 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     6841 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     6901 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6961 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7021 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7081 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7141 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7201 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     7261 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     7321 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     7381 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     7441 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7501 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7561 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7621 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7681 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7741 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7801 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7861 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7921 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7981 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8041 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     8101 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     8161 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8221 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8281 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8341 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8401 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8461 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8521 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8581 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8641 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8701 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8761 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8821 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8881 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8941 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     9001 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     9061 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9121 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9181 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc
     9241 tctagagatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcacg gacttgtggg
     9301 agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata
     9361 gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta
     9421 catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag ttataaacag
     9481 cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataagtatcc
     9541 cttggagaac caccttgttg gaccaggatg ggcaccaccc gtttaagagc taagctggaa
     9601 acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     9661 cggtgctttt tt
//
