LOCUS       sequence-256440-        1410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyCatcher003 for display at the cell surface of mammalian
            cells, with superfolder GFP for visualization and myc tag for 
            antibody detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, 
            Hytonen VP, Howarth M
  TITLE     Approaching infinite affinity through engineering of peptide-protein
            interaction.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116.
  PUBMED    31822621
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116."
COMMENT     Sequence Label: TfR-sfGFP-myc tag-SpyCatcher003
FEATURES             Location/Qualifiers
     source          1..1410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             283..996
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             997..1026
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1054..1392
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
ORIGIN
        1 atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatgt
       61 acccgggcca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt
      121 gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa
      181 aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga
      241 tttatgattg gctacttggg ctatggaagc ggaggatccg ggatgcgtaa aggcgaagag
      301 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      361 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      421 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      481 ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc
      541 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
      601 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
      661 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
      721 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
      781 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
      841 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
      901 ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca
      961 gcgggcatca cgcatggtat ggatgaacta tacaaagaac aaaaactcat ctcagaagag
     1021 gatctgggtt ccggtggctc gggtggatcc atggtgacca cactgtccgg actgtctgga
     1081 gagcagggac catccggcga catgaccaca gaggaggatt ctgccacaca catcaagttc
     1141 agcaagaggg acgaggacgg aagagagctg gcaggagcaa ccatggagct gagggatagc
     1201 tccggcaaga ccatcagcac atggatctcc gacggccacg tgaaggattt ctacctgtat
     1261 cccggcaagt acacctttgt ggagacagca gcaccagacg gatatgaggt ggcaacccct
     1321 atcgagttta cagtgaacga ggacggacag gtgaccgtgg atggagaggc aacagagggc
     1381 gatgcacaca ccggatctag cggatcttaa
//
