LOCUS       sequence-256442-       10255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Gallardo T, Shirley L, John GB, Castrillon DH
  TITLE     Generation of a germ cell-specific mouse transgenic Cre line, 
            Vasa-Cre.
  JOURNAL   Genesis. 2007 Jun . 45(6):413-7.
  PUBMED    17551945
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Jun . 45(6):413-7."
COMMENT     Sequence Label: pVasa-Cre
FEATURES             Location/Qualifiers
     source          1..10255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             406..414
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     intron          5503..6074
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     6082..6101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     6129..6148
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6129..6147
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6195..6215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6216..7244
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     polyA_signal    7278..7399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7315..7334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7369..7388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7433..7450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7604..8192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7684..7703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8363..9154,9155..9223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8986..9005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9224..9328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9396..9414
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9452..9474)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9574..9593
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9660..10115)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9797..9818)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10009..10028
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(10241..10255,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10255,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtcgacgg cgcgcctgtg ccaccatgcc tggcccagtt
       61 tcttgctttg tattataaac tttatagctg gtaagactct ggacccacac gtgtttgatt
      121 tgtctcttct ctcctgagac attttttccc agtaacattt cctgaatttt gtattttatt
      181 gtctctgcat ctgtgctgag acataacaac ttctgtttct tctatttgcc atgaactggg
      241 agttaagtct aagaatttga ttaaagctga agttgagttt gaaatcaaac ctctacatgt
      301 agcgttttgt atttcccact gtgatacatc aaccagcaca tggttcttgg aggtctttgc
      361 tttgtggtat aaagttggcc cagtgtgttt aaatgttgtc ctctttaagg aggtagctag
      421 gtaagatcac acgccggtta cctacctccc tactgatgca ctaactggtg tgcctagcct
      481 agcctaaggc cctagggtac catgcatccc ataatagctt atgtgaggcc caacacattt
      541 gtagataaca gcatcaggtc atagtgtcat aaggttggac acccctggca gagtttcatg
      601 caccacacta tcttcaaaag agctaaagaa gtaaggtgga aaaaacaaac atcatagtgt
      661 tctggtttta ctgtgacagg aaaagactca ctgaaaaggt gccaaggccc agatttcccc
      721 tcccacaatt ccaagcttca tggagaatga tacctccact ttggggatgg agaagggatt
      781 aaatcctgac cacagacttg aagcagtaag aacttatcac cgtgatgtca gttctgggtg
      841 gaaccctatg catcccgtgg cccagtacct gcagatgctg ctgctggacc tgtgatagat
      901 ggccagtact acagttagga atctttccga caactgcagc ttttagaatg gaaagcaaaa
      961 ctccacctac tggatggcac aacatgaaag caagggctgt gccttgcagc tcagggccag
     1021 ccttagtact agtctctgag ggaccgttgc taccaccggt tcccaacctg tagcagcaca
     1081 actatcctgg accagggttc acggatggag ctcaagacct tgacatctgt cacagttatg
     1141 ctctgcacca tgaggaagtt gtctcagttt gccttactgc cacagcaccg gctgtgggct
     1201 cagcattgtg acactcagag ccacaggtct ggagcaggaa ccagggcagg cccctctggt
     1261 gattctttct acagttcacg accagaggct gttggctttg gaaggcacct ggaggtctgt
     1321 gcaagccccg gacaaaggct tgtgtagatg aaggaccctt tataaaaagc tccctaattg
     1381 agtcttgaaa aactcacccg ctgaggattt aggagaaacc taagctcgga tcgcctccta
     1441 acactgccac aggtaacctg aattttggtg ccatatagtg ttagaaacta taggctggga
     1501 gagagaaaaa aaaaacattc ttttttcaat ttctgaaaac aaacaaaacc aaaacaagcc
     1561 atattatgaa gaccggaata aatacctaat cctttgtcct cgaatgacat cacgcatcaa
     1621 taagaaccaa ggatgtttaa ggaactatga cctcatctga cagtataaat ataaaatgcc
     1681 agaaaccgtc aagatagtaa ccagtgtggg tggatgctca tatgtgtact gtattagtta
     1741 ctttttcatt gtcgtgataa aataccacga ctaaagcctg acttatagga gaaggaattt
     1801 gccctgtgtt ttcagagtga taagactcca tcatggcagg gacgcatgga agccagcaac
     1861 aggtatgatg ctggggcagc aagctgagag ctcacatccg ccactgaagg cacaaaacag
     1921 caagtgtgag ctggaagtgg tgtgaggcta tatactagca aaggctgccc tactgatacg
     1981 ctgtctccag gaggactgcg ttccccaagc ttctctgatc atcacaacca actgaggact
     2041 aaatgttcaa atacctgggt cagtggggat catttctcat tcaaacttcc acagataatt
     2101 aaaagcaaat aaatgaacca ggcttaagga aacttactga attccaatag aaaatataga
     2161 aataaatcaa tactttagta gatataattg actgagacat gggggcaaat tttaaaaagt
     2221 cgaaattctt gataatgaag gtatactaaa caaaataaaa ttgcattagg gaagcattga
     2281 tgttagagta aatcaagcag aagagagaat gagtgaattc caagataggc catttgaaaa
     2341 tgcacagaag agaaaaagaa ttaagaggaa gaaatgtact catggtaacc ttggagcaac
     2401 agtggaaggg taagtaggtt agtggggata tggacgggac ttgagaacac caaaggagtt
     2461 aatcgatgca actacgttat gatcaaccac agtcaggttc caggttctgc agtaaactga
     2521 atctccagtt ttcacgctat gtagatccat ctccatgaat ctggcctggc cttgtgactt
     2581 tctggtaact gattagttga aagttgcaaa tataattgtt taacttctta tgctagaatt
     2641 caagaagcct tgcaactatt accctggttt ttcggagcga tgtccctgac aggtgtgtcc
     2701 agttgaagtg cagtatcctt acagagtcgg actgggattg tccaacaacc ttcagagcct
     2761 aagacagcct cttggaaact attcaattgt tctggccatc ccagccccta gatgccagag
     2821 tagagaagtc accttggatg ttaaacaaac tggtattgga ggttggggat aaactcagta
     2881 gtacagagct tgcctggcac gctcaagtac ctaggttcaa actctggcaa tgccaaaaaa
     2941 gaaaaaagtt gatagcaaac tctcagatga ttcaggttct agttagcgtt tgaatggaat
     3001 tgtatgagat ggtctcctga gccctctcaa tccctgagag agaatttttt tgtaagtcaa
     3061 tatattatgc aataatttgt tatatagttg tactggctaa tttgggtcaa gctggagtta
     3121 tcacagagaa aggagcttca gttggggaaa tgcctccatg agatccaact gtaaggcatt
     3181 ttctcaatta gtgatcaagg gggaaaggcc ccttgtgggt agggccatct ctgggctggt
     3241 agtcttggtt ctataagaga gcaggctgag caagccaggt gaggcaagcc agtaaggaac
     3301 atccctccat ggcctctgta tcagctcctg ctcctgacct gcttgagttc cagtcctgac
     3361 ttcctttggt gatgaacagc aatgtggaaa gtgtaagctg gataaaccct ttcctcccca
     3421 atttgcttag tggtcatgat gttttgtcca ggaatagaaa ccctgactaa gacaatagta
     3481 ataggtaaca agaagagagc tgtgaactcg tcatgtagtt aagataagtg tttgctagat
     3541 ttcctattgc atagttccta ttttctcttt atatttggaa aaaaattgtg gtaacatacc
     3601 ttggacactt attgttgttc tcccccctga cctgcactca atgctgaatt ctcagccaaa
     3661 ctctagtgat gtcctcccca ttcaaaagct tcaaccttcc agtctttatt taaaaaaaat
     3721 gttattatta catttactta ttctgtggtg tgtgtgtgtg tgtgagagag gatgggagca
     3781 ggtgtgtgcg tgtcggtgtg tttgttaaat cagaacatga gtgtgtgtgt aggacagaga
     3841 cggggtgagt gtgtgtgtgt gtgtatggga gcaggtgtgt gtgtgtgtgg gtcagtggat
     3901 aagttgtgga agtcagttct ttccttccct tactcggatc tcagggatca aacccaagtc
     3961 ctcaggttgc caggtacatg tcacctgtct ttgctttatt attatttagt cctggctttt
     4021 gagcctaagt gttatgttac ataggttgac cttgaattag ctatgcactg agctcctgat
     4081 cctcctgcct atatcttcca agtaatggtt acaggaatgg gccactggac ctagcaagtg
     4141 aacctacctg gtcactccaa aaatgcacag caaagaatat acgtttaaaa ataggttcat
     4201 tttaggaagt ttgtccacat tttaaatgac agttctgtta aagtatactg tgttttgtcc
     4261 tttgttaaat gtgactttta aaagcaattc accttaatag cctgggcgac tacagtgctc
     4321 actgtataaa tgctagtgtg tttttggtgc tgagacagaa aggtggctct agaaagctgg
     4381 agtccctcat ctttaagttc cagactgaga tatctagaac ttcttcaaaa atccagggaa
     4441 aggaaagcga tggaagtgaa aataaaaaca agaacatgct ttacatatat ttgattgtga
     4501 tccctttggc gggtactagg aaaaccacgg atggaatttt ccttcttgaa ggaaaggaaa
     4561 gccgaggagc aggcagaatg tgaacatcta cttaatgagc tgaactggcc ggtgccctca
     4621 gaattgtaaa caggttcacc acaaatccag gccttggcaa acggaccaag tcttcctctc
     4681 ttcggttttc tttttacaga ctggctttct tgacaacttc aagatggagt ctcatccttg
     4741 ccctttttat ggagaggaga agcattgctt ctagttggtt ttagtagagg agtgaagtgc
     4801 atttctcaga tacaaagaga gcacttgaga cgttcagact cagaatggcc aagcctggca
     4861 ctttgggagg tcaagaggag gctggaacgg ctgggagaga aagcaattag atgttccgcc
     4921 cctttggttt ttctccagac agggtttctc tgtgtagccc tgactgtcct gaagtttgct
     4981 ctgtagacca ggctggcctc cacccaggga tttgcctgcc tctgcctccc cgagccccag
     5041 atttttattt ttatttatat atatattaac ttttgaatga acacaatgga attgatgagc
     5101 ccttggagag tgaaacggga tgtcgtggga tgtcgtgcgt ggcagccccg gggatcagct
     5161 cactcccaca ggcctcacaa gccatggagc caagaggcct ccctgcctcg gcctcggcct
     5221 cggcctcggc ctcaacaaag gtggagaacg cgcaggccgt ccgtccatgg ggcgggaagt
     5281 cgcgcgccgc ggccgctgat tggctggcgg gcccggtcgc ctgatgctat ttgttgtccc
     5341 cgcgccaatg acgcagtcgg cgtcccggcg tccgcccgca cgtgcagccg tttaagccgc
     5401 gtcggccggc cgcgaggagc ccggggagcc tggagcggag agcggccgct taattaacta
     5461 ggcttttgca aaaagctccg gatcgatcct gagaacttca gggtgagttt ggggaccctt
     5521 gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc
     5581 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
     5641 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
     5701 tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt
     5761 gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt
     5821 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     5881 agaatatttc tgcatataaa ttctggctgc gtggaaatat tcttattggt agaaacaact
     5941 acatcctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     6001 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     6061 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     6121 aagaattgta atacgactca ctatagggcg aattcgcgtg tggcctcgaa caccgagcga
     6181 ccctgcagac catgcccaag aagaagagga aggtgtccaa tttactgacc gtacaccaaa
     6241 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
     6301 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
     6361 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
     6421 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
     6481 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
     6541 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
     6601 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
     6661 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
     6721 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
     6781 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
     6841 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
     6901 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
     6961 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
     7021 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
     7081 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
     7141 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
     7201 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagccatta acgcgaattc
     7261 ggatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7381 aactcatcaa tgtatcttat catgtctggt cgactctaga ctcttccgct tcctcgctca
     7441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9361 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9421 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9541 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9601 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     9661 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     9721 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     9781 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9841 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9901 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9961 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
    10021 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
    10081 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
    10141 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10201 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgtt
//
