LOCUS       sequence-256450-       12792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial nuclease-null SP Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 10901 to 10935)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcasN-
FEATURES             Location/Qualifiers
     source          1..12792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      274..301
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_RNA        10658..10736
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        10901..10935
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   10952..10987
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     rep_origin      11169..11907
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        11955..12046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12047..12792,1..46)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
       61 ttcaagccga ggggccgcaa gatccggcca cgatgacccg gtcgtcggtt cagggcaggg
      121 taccaggcac gcctaaccgt cagtgagatt ggatgagtga acgatattga tcgagaagag
      181 ccctgcgcag ccgctgccgt gcctgcagga agcaacggcc cggagggtgg cgggcaggac
      241 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
      301 ttgcgtttct acaaactctg ctagcttcta gagcacagct aacaccacgt cgtccctatc
      361 tgctgcccta ggtctatgag tggttgctgg ataactttac gggcatgcat aaggctcgta
      421 tgatatattc agggagacca caacggtttc cctctacaaa taattttgtt taacttttac
      481 tagaggagga ggcaaaaatg gacaagaagt actccattgg gctcgctatc ggcacaaaca
      541 gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc
      601 tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact
      661 ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca
      721 gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct aaggtggatg
      781 actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa aagcacgagc
      841 gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag tacccaacca
      901 tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg cggttgatct
      961 atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag ggggacctga
     1021 acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact tacaatcagc
     1081 ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc ctgagcgcta
     1141 ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga
     1201 acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac tttaaatcta
     1261 acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac gatgatgatc
     1321 tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg gcggcaaaga
     1381 acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag atcaccaaag
     1441 ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac ttgactttgc
     1501 tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc ttcgatcagt
     1561 ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa ttttacaaat
     1621 ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta aagcttaaca
     1681 gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc caccagattc
     1741 acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc tttttgaaag
     1801 ataacaggga aaagattgag aaaatcctca catttcggat accctactat gtaggccccc
     1861 tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag accatcactc
     1921 cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc atcgaaagga
     1981 tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt
     2041 acgagtactt cacagtttat aacggggttt gagggccaat ggaacgaaaa cgtacgttta
     2101 tggtataact tattgattat aatacagtat acaaaggtcc cttttcaggg accctttcgt
     2161 atgttgtagc tgattttatt ttcttctttt cttttgggtg cttttaatag ccatatatga
     2221 tgtttcatat aaagttaaaa gtttccccat ctgtctatct atagcgtcat gtttttcagg
     2281 atttcttaat ttctgcagca gtttagatat atatttcgca ggtaacttta ttattaatga
     2341 attatgggat tgatacattt cctttgtcca atctaaatct tttcctgatt tcaatacctc
     2401 actctcatct ctattaatta taaaaccgag ctttccatac ggacctgtta aatatgattg
     2461 taattgtcta tactcagatg gacctaattc ttcaaaattt ttagcatcaa aaacaacttg
     2521 tcttgtttta tagtcctcca atactcgttt ccaaaaatca gatttgccac cattggtgcc
     2581 tataatatct cgtctctgaa ctgcgttacc atttggatgg gacttgatgt ctgttaggtg
     2641 ggatgcaaat actattctta gtgcgtctaa acaccattgt tcaaattcag tggcaccttc
     2701 atttcctatc ggtatctgat ctagatgagt agttatttga cctattgttt tatttctaat
     2761 ggctgaatta tctgaaataa tattaatatc atattcgtca tttatctcct ctgcttcctc
     2821 tggagcaagt gcattacgat ttagattcaa accaagccag taacatggat gaattaataa
     2881 tttctcatta ctttcaaaac ctttatctgg agttcgcccg tcatggcaaa atgaatagga
     2941 tgaggtgttt ttatcacgaa taccaacaaa tcctacacta tacaaacttt ggagaattcc
     3001 acttgccttt aacaactgta tttcagacgt tattttagga tctccatttt cttcgattaa
     3061 ttcgaaagat gcttctattt tttttaagca cgtataaact gttaattcag gttcaatgct
     3121 acgaaatgca ctagttataa cctgtattga aggaaagatc ttctgatact ctttccagag
     3181 atcttcaagt ctggccatgg aaattgactt ggctgcatat tctaggtcag tgtttatgat
     3241 agtttctcta ttctctctga atgcggaaaa aaaagcttca ttcaacaatg atagtaaatc
     3301 cctgggccgg taaagggtaa attgcaaaca tcgcttaaaa ccattcctcc ctttaagatc
     3361 atccgctgtg catctatccc aaactcgttg atctttctca atatctagct taaatgctac
     3421 tttcattctt ttagctgaca gcattaggag ttgtgcccag tcccaatgca accttatgac
     3481 ttgaccctct atatttctcg agtaatcagg atcttccttt gatagcgacc taaatatatt
     3541 atcccttaaa aaaattattg gacgaatgca ttttgctttt tgatttaatt caatagatgc
     3601 atatgctaga cctgcaatga ttccaattcc tatattatcc ggttcatacg cctcatctag
     3661 cttatccatt aatatgacaa ctttcctgtc tgagcgttca agaagtgata ctatattatt
     3721 ttctatttct gagatattca aattgaattg aagatcacca attgattctt ctgggttatt
     3781 ttcatctaaa tactcctttg cgacaagcct acactttctt aaaatgtcac cttgtgcaga
     3841 attccatttt ttcaaatgtt cattcaacaa tgtttctgat gatatttgag atgacaattt
     3901 gtaatgagat gatatatatg atgctatctc cattagcata gcgtatcgcc ataatagtct
     3961 tgttgctgcc cttgctaaat taaatgatcc tgtaaatggt ttcaacattg atctgaaacc
     4021 aataatttga gaatcgtctg gtgagaaact caggattaat atttttttgt ctttcttcca
     4081 atgctcattt agctgaataa ataaagcact tttacctgtc cctcgtctac caacaacaat
     4141 ggtcctgtca tcagtttcaa ttagagtcct aaagtcagca gtttcaatga aagcattact
     4201 caacatcttt ttatcatttt ctgctgtcgt atcaccaaac ggattagact ttgaagtaat
     4261 attcaattcc atattcaacc ttttatgtta gttgctttca tttattactt tatatactgt
     4321 tgaacgagca atattcattg tttttgatat atgtgaggca cctaacccct gttgccacat
     4381 atttaatact gcatctctat ctatttttct ttttctacca aaaacaactc cttttgccat
     4441 tgcctcttgg cgaccttcat tggtacgctc tagtattctc tgtcgttctg cctgggccac
     4501 tgcagataga atagtgacaa ccattttacc catctcccca tcggtactga ttccgtcatc
     4561 aataaaccga atggatacac cttgggcgtc aaactctttt attaactgga tcatgtcagc
     4621 agtatcgcgc ccaaggcggt caagtttctt caccaagatg acgtcacctt cctccacctt
     4681 catcctcagc aagtccagcc ctttccgatc gcttgaactg cccgatgcct tgtcagtaaa
     4741 gatgcgattt gctttcacgc ctgcgtcttt gagtgcccga acctgaatat cgagagattg
     4801 ctggctggtt gatacccgtg cgtaaccaaa aagtcgcata aaaatgtatc ctaaatcaaa
     4861 tatcggacaa gcagtgtctg ttataacaaa aaatcgattt aatagacaca ttaacagcac
     4921 tgtttttatg tgtgcgataa tttataatat ttcggacggt tgcaaaattg ttaatgaata
     4981 actgtcaggt agggagcctg atatgcctgt cgactttcta acctctgacc aaaaacagaa
     5041 ttacggttgt tatgctgcag aacctaatga cgtgcaactg gcgcgctatt ttcatcttga
     5101 tgaacgggat ctggccttca ttaaccaacg acggggcaaa cataataggc tgggcattgc
     5161 gcttcagctc accacagccc gttttctggg aacatttctg acggatttaa ctcaggttct
     5221 gcctggtgtt caacattttg tcgcggtaca gcttaatatc caccgtccag aagttctctc
     5281 ccgctatgct gaacgggaca ctacccttag agaacatact gcattaatta aggaatatta
     5341 cggctatcat gaatttggtg attttccatg gtctttccgc ctgaagcgtc tgctatatac
     5401 ccgggcgtgg ctcagtaatg agcgaccggg tctgatgttt gattttgcca ctgcatggtt
     5461 gcttcaaaat aaggtattac tgcccggagc aaccacacta gtacgtctca tcagtgaaat
     5521 tcgtgaaagg gcaaatcagc ggctgtggaa aaagctggcc gcactgccga acaaatggca
     5581 ggcagctcaa gtgatggagc ttctggtcat tccggaaggt cagcgtgtat cagcactgga
     5641 acagttgaaa aaaggccctg ttacagtcag tggacctgcg tttaatgaag cgctggaacg
     5701 atatatccga ttacgaagtc ttgagttttc ccgactgaac ttttccggtc tgcctgccat
     5761 tcaactgcgt aatctggctc gttatgctgg catggcgtcg gtaaaatata tcgctcgaat
     5821 gccacagcag agaaagcttg ctgtacttac tgcattcgtt aaagcacagg aaataacggc
     5881 attagacgat gccgttgatg tgcttgatat gctaattctg gacattatcc gcgaagcaaa
     5941 gaaaaccggg caaaaaaaaa gactcaggac actgaaagat cttgatcagg ccgcattgtt
     6001 actggcgcgg gcatgtgcat tgttgctgga tgataataca gatgtcccag atctcaggca
     6061 ggttatcttc aagtgcgtac ccaaaaacag actggcagaa tctgtaagca aggttaatga
     6121 acttgctcgt ccacagaaca ataatttcca tgacgaaatg gttgagcagt acggtcgggt
     6181 taaacgtttt cttccggcgg tgttgcggga cctgcatttc cgtgcggcac cggcaggtga
     6241 acatgtactg gctgcgattc attatctggc agaactgaat ggttcgaaaa agcgcatcct
     6301 tgatgatgcg cctgaacata ttatcaccgg tccctggaaa cgcctcgtat acgatgcgga
     6361 gggacggata cagcgtgcag gttattcact atgtttgctg gaacgccttc aggatgcact
     6421 gcgccgccgg gacatctggc ttgaaaacag tgatcgctgg ggagatcctc gcgagaagtt
     6481 gttgcaaggt gaagagtggc agactcagcg tattcctgtc tgtcgggcac tgggacatcc
     6541 tgtcgatgga cgtaaaggtg tgcaacaact ggctattcag ctggatgaga cctggaaagc
     6601 cgtggcatca cgatttgaaa agaatgcgga agttcatatc tgtaatgaag gtaaatatcc
     6661 atccctgact atcagttgtc tggagaaaca ggaagagcca ccatcattgc ttcgtctaaa
     6721 taatcggatc aaacagctac tcccaccggt agatttaacg gaactgttac ttgagataga
     6781 tgcccagaca ggatttacac atgagtttgc gcatgtcagt gaatctggtg ctcgagcgca
     6841 agatttgcac atcagtttat gtgcggtatt gatggctgaa gcctgtaata tcggactgga
     6901 accgctgata aagcacaata taccagcact gacccgccat cggctcagtt gggtgaaaca
     6961 gaattacctt cgtgcagaaa cgctggtcag cgccaatgcc cgcctggttg attttcagtc
     7021 cacactggag cttgctggtc gttggggagg tggagaagtg gcatcagctg acggcatgcg
     7081 ctttgtcaca ccagtgaaga ccatcaactc aggatctaac agaaaatatt ttggttctgg
     7141 acgaggcatc acctggtata acttcgtatc tgatcagtac tctgggttcc atggcattgt
     7201 ggtacccggt acattacggg attcgatttt tgtactggaa ggacttcttg agcagcagac
     7261 agggctgaat ccagttgaaa tcatgacaga cactgcgggt agcagcgata ttattttcgg
     7321 tctgttctgg ctactggggt atcagttttc cccccggctt gccgatgcag gtgaggcggt
     7381 gttctggcgg gtgaataaat cggcaaacta cggagtactg gataagttgg cccgaggata
     7441 tgcagatctg tcaaaagcgg agagtcagtg ggatgagatg atgcgaaccg ctggttcgct
     7501 gaagttgggt acaattcatg cgtcagaact cattcgctct ttactgaaaa gctcgcgccc
     7561 atcaggactg gcacaggcaa ttatggaagt agggcgtgta aacaaaacgc tatatctcct
     7621 caattatatt gatgatgaag attatcgtcg gcggatcctg acgcagctta atcgaggaga
     7681 aggccgccac gctgtggctc gtgcgatttg ttacggacaa cgcggagaga tcagaaagcg
     7741 ttatcgtgaa gggcaggaag atcaactggg ggcgctgggc ctggttacta atgcagtggt
     7801 tctgtggaac acgctctata tggaggaagc gttgagctgg atgcgccgta atggcgaaga
     7861 aattatagat gaagatatcg ctcggctatc tcccctgatg cacgggcata tcaatatgtt
     7921 gggccattat acattcacgt tgccagagga tattttaaaa ggggaactga gagctctaaa
     7981 tttaaatata aacaacgaat tatctcctta acgtacgttt tcgttccatt ggccctcaaa
     8041 ccccataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
     8101 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     8161 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     8221 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     8281 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     8341 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     8401 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     8461 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     8521 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     8581 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     8641 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     8701 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     8761 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     8821 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     8881 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     8941 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggct
     9001 gctatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     9061 tccgataaag ctagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     9121 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     9181 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     9241 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     9301 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     9361 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     9421 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     9481 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     9541 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     9601 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     9661 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     9721 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     9781 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     9841 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     9901 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     9961 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
    10021 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
    10081 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
    10141 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
    10201 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
    10261 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
    10321 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
    10381 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
    10441 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
    10501 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
    10561 atcgacctct ctcagctcgg tggagactaa ccggctgata aatttctttg aatttctcct
    10621 tgattatttg ttataaatgt tataaaataa tcttgttgga accattcaaa acagcatagc
    10681 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
    10741 tttgatactt ctattctact ctgactgcaa accaaaaaaa caagcgcttt caaaacgctt
    10801 gttttatcat ttttagggaa attaatctct taatcctttt atcattctac atttaggcgc
    10861 tgccatcttg ggacaatgaa aacgttagtc atggcgcgcc ttgacggcta gctcagtcct
    10921 aggtacagtg ctagcttaat tagtctacga ggttttagag ctatgctgtt ttgaatggtc
    10981 ccaaaacctg cagacaagcc cggccggcct aaggcgatgc cccctcgacc tcgatcaggg
    11041 aggcgttcag gacgactcac aaagaaagcc gggcaatgcc cggctttttc cacgcctcct
    11101 gggctgactt caggtgctac atttgaagag ataaattgca ctgaaatcta gagcggttca
    11161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct
    11221 gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact
    11281 gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta
    11341 gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg
    11401 aactcccagt taccaatggc tgctgccagt ggcgttttac cgtgcttttc cgggttggac
    11461 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta
    11521 cagcccagct tggagcgaac gacctacacc gagccgagat accagtgtgt gagctatgag
    11581 aaagcgccac acttcccgta agggagaaag gcggaacagg tatccggtaa acggcagggt
    11641 cggaacagga gagcgcaaga gggagcgacc cgccggaaac ggtggggatc tttaagtcct
    11701 gtcgggtttc gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac
    11761 cggaaaagcg tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca
    11821 ccggcgcggc cctgctgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt
    11881 gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc
    11941 gagtgagcta gctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    12001 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gggaagcggt
    12061 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
    12121 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
    12181 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
    12241 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
    12301 tgtagaagtc accattgttg tgcacgacga catcattcct tggcgttatc cagctaagcg
    12361 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcgggtatct tcgagccagc
    12421 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
    12481 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
    12541 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
    12601 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
    12661 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
    12721 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
    12781 gttggaagaa tt
//
