LOCUS       sequence-256480-        7384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7384)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmocollin1-N-18
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      617..626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3353..3362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3359..4069
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3504..3522)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3772..3791
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4192..4313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4229..4248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4283..4302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4320..4775)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4457..4478)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4669..4688
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4802..4906
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4908..5265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4908..5099
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4931..4951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5116..5251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5300..6094
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5300..6094
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5354..5373)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5964..5983
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6282..6301)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6326..6373
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6702..7290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7191..7210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      601 cgtcagatcc gctagcgcca ccatggctct ggcctctgct gccccaggga gcatcttctg
      661 taagcagctc cttttctctc tcctggtttt aacattactt tgcgatgctt gtcagaaagt
      721 ttatcttcga gttccttctc atcttcaggc tgaaacactt gtaggcaaag tgaatctgga
      781 ggagtgtctc aagtcggcca gcctaatccg gtccagtgac cctgccttca ggattctaga
      841 agatggctca atttacacaa cacatgacct cattttgtct tctgaaagga aaagtttttc
      901 cattttcctt tcagatggtc agagacggga acaacaagag ataaaagttg tactgtcagc
      961 aagagaaaac aagtctccta agaagagaca taccaaagac acagccctca agcgcagcaa
     1021 gagacgatgg gctcctattc cagcttcatt gatggagaac tcgttgggtc catttccaca
     1081 acacgttcag cagatccaat ctgatgctgc acagaattac accatctttt attccataag
     1141 tgggccaggc gtggacaaag aacccttcaa tttgttttac atagagaaag acactgggga
     1201 tatcttttgt acaaggagca ttgaccgtga gaaatacgaa cagtttgcgt tatatggcta
     1261 tgcaacaact gcagatggct atgcaccaga atatccactc cctttgatca tcaaaattga
     1321 agatgataat gataacgccc catattttga acacagagtg actatcttta ctgtgcctga
     1381 aaattgccga tccggcactt cagtgggaaa agtgaccgcc acagaccttg acgaacctga
     1441 cactctccat actcgtctga aatataaaat cttacaacaa atcccagatc atccaaagca
     1501 tttctccata cacccagata ccggagtcat caccacaact acaccttttc tggatagaga
     1561 aaaatgtgat acttaccagt taataatgga agtgcgagac atgggtggtc agcctttcgg
     1621 tttatttaat acaggaacaa ttactatttc acttgaggat gaaaatgaca atccaccatc
     1681 tttcacagaa acttcttatg ttacagaagt agaagaaaac agaattgacg tggagatttt
     1741 gcgaatgaag gtacaggatc aggatttgcc aaacactcct cactcaaagg ctgtatacaa
     1801 aatcttacaa ggaaatgaaa atggaaactt cataattagc acagatccaa atacaaatga
     1861 aggagtgctg tgtgttgtca agccattgaa ctatgaagtc aatcgccaag ttattttgca
     1921 agttggtgtc attaacgagg cacaattctc taaagcagcg agctcacaaa ctcctacaat
     1981 gtgcactaca actgtcaccg ttaaaattat agacagtgat gagggccctg aatgccaccc
     2041 tccagtgaaa gttattcaga gtcaagatgg cttcccagct ggccaagaac tccttggata
     2101 caaagcactg gacccggaaa tatccagtgg tgaaggctta aggtatcaga agttagggga
     2161 tgaagataac tggtttgaaa ttaatcaaca cactggcgac ttgagaactc taaaagtact
     2221 agatagagaa tccaaatttg taaaaaacaa ccaatacaat atttcagttg ttgcagtgga
     2281 tgcagttggc cgatcttgca ctggaacatt agtagttcat ttggatgatt acaacgatca
     2341 cgcacctcaa attgacaaag aagtgaccat ttgtcagaat aatgaggatt ttgctgttct
     2401 gaaacctgta gatccagatg gacctgaaaa tggaccacct tttcaattct ttctggataa
     2461 ttctgccagt aaaaactgga acatagaaga aaaggatggt aaaactgcca ttcttcgtca
     2521 acggcaaaat cttgattata actattattc tgtgcctatt caaataaaag acaggcatgg
     2581 tttagttgca acacatatgt taacagtgag agtatgtgac tgttcaactc catctgagtg
     2641 tagaatgaag gataaaagta caagagacgt tagaccaaat gtaatacttg gaagatgggc
     2701 tattcttgct atggtgttgg gttctgtatt gttattatgt attctgttta catgtttctg
     2761 tgtcactgct aagagaacag tcaagaaatg ttttccagaa gacatagccc agcaaaattt
     2821 aattgtatca aatactgaag gacctggaga agaagtaacg gaagcaaata ttagactccc
     2881 catgcagaca tccaacattt gtgacacaag catgtctgtt ggtactgttg gtggccaggg
     2941 aatcaaaaca cagcaaagtt ttgagatggt caaaggaggc tacactttgg attccaacaa
     3001 aggaggtgga catcagacct tggagtccgt caagggagtg gggcagggag atactggcag
     3061 atatgcgtac acggactggc agagtttcac ccaacctcgg cttggcgaaa aggtgtattt
     3121 gtgtggacaa gatgaggagc ataaacattg tgaagactac gtttgttcgt ataactatga
     3181 aggcaaaggt tctctggccg gctcagtagg ttgctgcagc gatcggcagg aagaagaggg
     3241 actggagttt ctagatcacc tggaacccaa atttaggaca ttagcaaaga catgcatcaa
     3301 gaaaagcagc ggtggagcaa gcgcagccag tggtagcgcg gatccaccgg tcgccaccat
     3361 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3421 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3481 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3541 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3601 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3661 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3721 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3781 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3841 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3901 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3961 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4021 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga
     4081 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     4141 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     4321 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     4381 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     4441 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     4501 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     4561 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     4621 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     4681 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     4741 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     4801 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4861 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     4921 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4981 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5041 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5221 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     5281 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5341 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5401 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5461 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5521 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5581 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5641 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5701 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5761 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5821 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5881 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5941 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6001 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6061 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6121 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6181 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6241 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     6301 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     6361 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6421 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6481 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     6541 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6841 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7321 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7381 gata
//
