LOCUS       sequence-256486-        4713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM
  TITLE     mKikGR, a monomeric photoswitchable fluorescent protein.
  JOURNAL   PLoS One. 2008;3(12):e3944. doi: 10.1371/journal.pone.0003944. Epub 
            2008 Dec 15.
  PUBMED    19079591
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008"; volume: "3(12):e3944. doi"; pages: " 
            10.1371/journal.pone.0003944. Epub 2008 Dec 15"
COMMENT     Sequence Label: mKikGR-C1
FEATURES             Location/Qualifiers
     source          1..4713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        344..547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     498..518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      586..595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             592..1290
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     misc_feature    1291..1356
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1480..1601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1517..1536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1571..1590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1608..2063)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1745..1766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1957..1976
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2090..2194
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2196..2553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2196..2387
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2219..2239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2404..2539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2588..3382
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2588..3382
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2642..2661)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3252..3271
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3570..3589)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3614..3661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3990..4578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4479..4498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
       61 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      121 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      181 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      241 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      301 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      361 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      421 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      481 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      541 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagt
      601 gtgattacat cagaaatgaa gatcgagctg cgtatggaag gctctgtaaa cgggcacaag
      661 ttcgtgatta cagggaaagg aagtggcaga ccttacgagg gaacacagac tgtggacctg
      721 acagtcatag agggcggacc tcttcctttt gctttcgata tcctgacaac agcattccat
      781 tacggcaacc gggtatttgt cgaataccca gaagaaatag tagactactt caagcagttg
      841 tttcctgagg gttattcttg ggaacgaagc atgagttacg aagacggggg aatttgcctc
      901 gccacaaaca atataacgat gaagaaagac ggcagcaaca cttttgtcaa tgaaattcga
      961 tttgatggta caaactttcc tgccaatggt ccagttatgc agaggaagac cgtcaaatgg
     1021 gagccatcca ctgagaaaat gtatgtgcgt gatggagtgc tgaagggtga tgttgaaatg
     1081 gctctgttgc ttgaaggagg tggccattac cgatgtgact tcagaactac ttacaaagca
     1141 aagaaggttg tccagttgcc agactatcac tacgtggatc atcaaatgga gataacaagc
     1201 catgacaagg attacaacaa ggttaaggcg tatgaacatg ctaaggctta ttccgggacc
     1261 tacaggggcg ccaagtatga atttgaagcc tccggactca gatctcgagc tcaagcttcg
     1321 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
     1381 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     1441 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1561 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     1621 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1681 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     1741 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     1801 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     1861 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     1921 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     1981 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2041 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2161 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     2221 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2281 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     2341 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2461 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2521 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     2581 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2641 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2701 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2761 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2821 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     2881 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     2941 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     3001 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     3061 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     3121 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     3181 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     3241 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     3301 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     3361 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     3421 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3481 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3541 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     3601 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     3661 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     3721 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     3781 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     3841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4681 accgccatgc attagttatt aatagtaatc aat
//
