LOCUS       sequence-256579-        9300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid to co-express a guide RNA and TagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Giuliano CJ, Lin A, Girish V, Sheltzer JM
  TITLE     Generating Single Cell-Derived Knockout Clones in Mammalian Cells 
            with CRISPR/Cas9.
  JOURNAL   Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
  PUBMED    31503414
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2019-09"; volume: "128(1):e100. doi"; pages: " 
            10.1002/cpmb.100"
COMMENT     Sequence Label: LRG2.1-TagBFP2
FEATURES             Location/Qualifiers
     source          1..9300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..66
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     316..336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        318..336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        364..590
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             591..771
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    818..943
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1436..1669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1854..1898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2076..2324
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2076..2096
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2247..2266
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4350..4467
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4536..4747
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4767..4776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4776..4799
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4800..5501
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5516..6104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5569..5589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5987..5998)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6176..6409
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6487..6608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6524..6543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6578..6597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6648..6783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6803..6822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6804..6822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6832..6849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6832..6848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6841..6863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6990..7445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7077..7096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7287..7308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7471..7575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7576..7644,7645..8436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7794..7813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8607..9195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9096..9115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      301 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
      361 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
      421 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
      481 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
      541 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
      601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      781 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
      841 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      901 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      961 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1021 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1081 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1141 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1201 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1261 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1321 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1381 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1441 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1501 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1561 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1621 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1681 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1741 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1801 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1861 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1921 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1981 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2041 ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt
     2101 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2161 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2221 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2281 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac ggttgtaaat
     2341 gagcacacaa aatacacatg ctaaaatatt atattctatg acctttataa aatcaaccaa
     2401 aatcttcttt ttaataactt tagtatcaat aattagaatt tttatgttcc tttttgcaaa
     2461 cttttaataa aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc gttgttttct
     2521 tcacctttaa taatagctac tccaccactt gttcctaagc ggtcagctcc tgcttcaatc
     2581 attttttgag catcttcaaa tgttctaact ccaccagctg ctttaactaa agcattgtct
     2641 ttaacaactg acttcattag tttaacatct tcaaatgttg cacctgattt tgaaaatcct
     2701 gttgatgttt taacaaattc taatccagct tcaacagcta tttcacaagc tttcatgatt
     2761 tcttcttttg ttaataaaca attttccata atacatttaa caacatgtga tccagctgct
     2821 ttttttacag ctttcatgtc ttctaaaact aattcataat ttttgtcttt taatgcacca
     2881 atatttaata ccatatcaat ttctgttgca ccatctttaa ttgcttcaga aacttcgaat
     2941 gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta caacatttgt tattcctaca
     3001 tttgtgcctt ttaataattc tttacaatag cttgttcaat atgaattaac acaaactgtt
     3061 gcaaaatcaa attcaattgc ttcatcacat aattgtttaa tttcagcttt cgtagcatct
     3121 tgttttaata atgtgtgatc tatatatttg tttagtttca ttttttctcc tatatattca
     3181 tttttaattt taattcttta ataatttcgt ctactttaac tttagcgttt tgaacagatt
     3241 caccaacacc tataaaataa atttttagtt taggttcagt tccacttggg cgaacagcaa
     3301 atcatgactt atcttctaaa taaaatttta gtaagtcttg tcctggcata ttatacattc
     3361 catcgatgta gtcttcaaca ttaacaactt taagtccagc aatttgagtt aagggtgttg
     3421 ctctcaatga tttcattaat ggttcaattt ttaatttctt ttcttctggt ttaaaattca
     3481 agtttaaagt gaaagtgtaa tatgcaccca tttctttaaa taaatcttct aaatagtcta
     3541 ctaatgtttt attttgtttt ttataaaatc aagcagcctc tgctattaat atagaagctt
     3601 gtattccatc tttatctcta gctgagtcat caattacata tccataactt tcttcataag
     3661 caaaaacaaa atttaatccg ttatcttctt ctttagcaat ttctctaccc attcatttaa
     3721 atccagttaa agtttttaca atattaactc catatttttc atgagcgatt ctatcaccca
     3781 aatcacttgt tacaaaactt gaatatagag ccggattttt tggaatgcta tttaagcgtt
     3841 ttagatttga taattttcaa tcaattaaaa ttggtcctgt ttgatttcca tctaatctta
     3901 caaaatgacc atcatgtttt attgccattc caaatctgtc agcatctggg tcattcataa
     3961 taataatatc tgcatcatgt ttaataccat attcaagcgg tatttttcat gcaggatcaa
     4021 attctggatt tggatttaca acatttttaa atgtttcatc ttcaaatgca tgctcttcaa
     4081 cctcaataac gttatatcct gattcacgta atatttttgg ggtaaattta gttcctgttc
     4141 cattaactgc gctaaaaata atttttaaat cttttttagc ttcttgctct tttttgtacg
     4201 tctctgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     4261 tcaactttgc tggaaacagc aaagtggcac cgagtcggtg ctttttttga attctagatc
     4321 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4381 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4441 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4501 tggcgccggc tcgagtggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4561 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     4621 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4681 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4741 aacacaggtg tcgtgacgcg ggatccgcca ccatggatta caaagacgat gacgataaga
     4801 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     4861 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4921 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     4981 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     5041 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     5101 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     5161 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     5221 aggccttcac cgaaacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     5281 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     5341 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     5401 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     5461 actgcgacct ccctagcaaa ctggggcaca agcttaattg acgcgttaag tcgacaatca
     5521 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5581 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5641 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5701 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5761 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5821 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5881 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5941 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6001 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6061 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6121 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6181 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6241 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6301 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6361 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     6421 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6481 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6601 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6661 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6721 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6781 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6841 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6901 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6961 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7441 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7501 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7561 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7621 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7681 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7741 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7801 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7861 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7921 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7981 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8041 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8101 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8161 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8221 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8281 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8341 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8401 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
//
