LOCUS       sequence-256588-       10464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES 
            eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Labuhn M, Perkins K, Matzk S, Varghese L, Garnett C, Papaemmanuil E,
            Metzner M, Kennedy A, Amstislavskiy V, Risch T, Bhayadia R, 
            Samulowski D, Hernandez DC, Stoilova B, Iotchkova V, Oppermann U, 
            Scheer C, Yoshida K, Schwarzer A, Taub JW, Crispino JD, Weiss MJ, 
            Hayashi Y, Taga T, Ito E, Ogawa S, Reinhardt D, Yaspo ML, Campbell 
            PJ, Roberts I, Constantinescu SN, Vyas P, Heckl D, Klusmann JH
  TITLE     Mechanisms of Progression of Myeloid Preleukemia to Transformed 
            Myeloid Leukemia in Children with Down Syndrome.
  JOURNAL   Cancer Cell. 2019 Aug 12;36(2):123-138.e10. doi: 
            10.1016/j.ccell.2019.06.007. Epub 2019 Jul 11.
  PUBMED    31303423
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.06.007"; journalName: "Cancer Cell"; date: 
            "2019-08-12- 12"; volume: "36"; issue: "2"; pages: "123-138.e10"
COMMENT     Sequence Label: LeGO-iG-CSF2RB(A455D)
FEATURES             Location/Qualifiers
     source          1..10464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2614..2630
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2672..2705)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2776..3182
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5500..5511
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(5911..6485,6486..6488,6489..6497)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6078..6095)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6305..6324
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6498..6500,6501..6503,6504..7217)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6498..6500,6501..6503,6504..7214)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6543..6564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6804..6823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7151..7172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7264..7297)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7353..7941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7406..7426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7824..7835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7944..7960)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8470..8650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8712..9300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8792..8811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9471..10262,10263..10331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10094..10113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10332..10436)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgccgaggtc gacggtatcg ataagctcgc
     2641 ttcacgagat tccagcaggt cgagggacct aataacttcg tatagcatac attatacgaa
     2701 gttatattaa gggttccaag cttaagcggc cggccgctga aagaccccac ctgtaggttt
     2761 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
     2821 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
     2881 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     2941 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
     3001 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     3061 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     3121 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgattgac tgagtcgccc
     3181 ggatccatgg tgctggccca gggcctgctg tctatggccc tgctggccct gtgctgggag
     3241 agaagcctcg caggcgccga ggaaaccatc cccctgcaga ccctgcggtg ctacaacgac
     3301 tacaccagcc acatcacctg tagatgggcc gacacccagg acgcccagcg gctggtcaac
     3361 gtgaccctga tcagaagagt gaacgaggac ctgctggaac ccgtgtcctg cgacctgagc
     3421 gacgacatgc cttggagcgc ctgcccccac cctagatgtg tgcctcggag atgcgtgatc
     3481 ccctgccaga gcttcgtggt caccgacgtg gactacttca gcttccagcc cgaccggcct
     3541 ctgggcacca gactgaccgt gacactgacc cagcacgtgc agccccccga gcccagggat
     3601 ctccagatct ctaccgacca ggaccacttc ctgctgactt ggagcgtggc cctcggcagc
     3661 cctcagagcc attggctgag ccctggcgac ctggaattcg aggtggtgta caagcggctg
     3721 caggacagct gggaggacgc tgccatcctg ctgagcaaca ccagccaggc caccctgggc
     3781 cccgagcacc tgatgcctag cagcacctac gtggccagag tgcggaccag actggcccct
     3841 ggcagcaggc tgagcggcag acctagcaag tggtcccccg aagtgtgctg ggacagccag
     3901 ccaggcgacg aggcccagcc ccagaacctg gaatgcttct tcgacggcgc tgccgtgctg
     3961 agctgcagct gggaagtgcg gaaagaggtg gccagcagcg tcagcttcgg cctgttctac
     4021 aagcccagcc ccgacgccgg cgaagaggaa tgcagcccag tgctgagaga gggcctgggc
     4081 tccctgcaca cccggcacca ctgtcagatc cccgtgcccg atcctgccac ccacggccag
     4141 tacatcgtgt ccgtgcagcc cagacgggcc gagaagcaca tcaagagcag cgtgaacatc
     4201 cagatggccc ctcccagcct gaacgtgacc aaggacggag acagctacag cctgagatgg
     4261 gagacaatga agatgcgcta cgagcacatc gaccacacct tcgagatcca gtaccggaag
     4321 gacaccgcca cctggaagga cagcaagacc gagacactgc agaacgccca cagcatggcc
     4381 ctgcccgccc tggaacccag caccagatac tgggccagag tccgcgtgcg gacctccaga
     4441 accggctaca acggcatttg gagcgagtgg tctgaggcca gaagctggga caccgagagc
     4501 gtgctgccta tgtgggtgct ggctctgatc gtgatcttcc tgacaatcga tgtgctgctg
     4561 gcactgcggt tctgcggcat ctacggctac cggctgcggc ggaagtggga ggaaaagatc
     4621 cccaacccca gcaagagcca cctgttccag aacggctccg ccgagctgtg gcctcctggc
     4681 tccatgagcg ccttcaccag cggctctcca ccccaccagg gcccttgggg cagcagattt
     4741 cctgagctgg aaggcgtgtt ccccgtgggc ttcggcgaca gcgaggtgtc ccctctgacc
     4801 atcgaggacc ccaagcacgt gtgcgacccc cccagcggac ctgatacaac ccctgccgcc
     4861 agcgacctgc ctaccgagca gcctccatct ccccagcctg gacctccagc cgccagccac
     4921 acaccagaga agcaggccag cagcttcgac ttcaacggcc cctacctggg ccctccccac
     4981 agcagaagcc tgcccgatat cctcggccag cccgagcctc ctcaggaagg cggcagccag
     5041 aagtcccctc cacccggcag cctggaatac ctgtgtctgc ctgccggcgg acaggtgcag
     5101 ctggtgcctc tcgctcaggc catgggacct ggacaggctg tggaagtgga aagaaggcct
     5161 agccagggcg ctgccggctc cccatctctg gaatctggcg gaggacctgc ccctcctgcc
     5221 ctgggaccta gagtgggcgg ccaggaccag aaagacagcc ccgtggccat ccccatgagc
     5281 agcggcgata cagaggaccc tggcgtggcc agcggctacg tgtccagcgc cgatctggtg
     5341 ttcaccccca acagcggcgc cagctccgtg tccctggtgc cttctctggg cctgcccagc
     5401 gaccagaccc ctagcctgtg tcctggactg gcctctggac ctcctggcgc ccctggacct
     5461 gtgaagtccg gcttcgaggg ctacgtggaa ctgcccccca tcgagggcag aagcccccgg
     5521 tcccccagaa acaaccctgt gccccctgag gccaagagcc ccgtgctgaa tcccggcgaa
     5581 agacccgccg acgtgtcccc cacaagccct cagccagagg gactgctggt gctgcagcaa
     5641 gtgggagact actgcttcct gcctggcctc ggaccagggc cactgtccct gagaagcaag
     5701 cctagctctc ccggcccagg ccccgagatc aagaacctgg accaggcctt ccaggtcaaa
     5761 aagcctcccg gacaggccgt gccccaggtg cccgtgatcc agctgttcaa ggccctgaag
     5821 cagcaggact acctgagcct gcccccctgg gaagtgaaca agcccggcga agtctgctga
     5881 gcggccgcta cgtaaattcc gccccccccc cccctctccc tccccccccc ctaacgttac
     5941 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6001 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6061 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6121 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6181 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6241 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6301 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6361 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6421 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6481 ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6661 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6961 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7081 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7201 acgagctgta caagtaaagc ggccggccgc cagcacagtg gtcgaaattc gtcgagggac
     7261 ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc
     7321 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7381 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7441 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7501 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7561 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7621 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7681 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7741 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7801 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7861 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7921 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7981 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8041 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8101 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8161 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8221 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     8281 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     8341 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     8401 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     8461 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8521 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8581 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8641 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8881 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9121 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10441 tttccccgaa aagtgccacc tgac
//
