LOCUS       sequence-256595-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
  TITLE     Tracing Information Flow from Erk to Target Gene Induction Reveals 
            Mechanisms of Dynamic and Combinatorial Control.
  JOURNAL   Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 
            10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
  PUBMED    28826673
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.07.016"; journalName: "Mol Cell"; date: 
            "2017-09-7- 7"; volume: "67"; issue: "5"; pages: "757-769.e5"
COMMENT     Sequence Label: pAAV Btg2 YFP MS2
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6157..6612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6244..6263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6454..6475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6629..6648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6748..6770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6808..6826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6894..6998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6999..7067,7068..7859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7217..7236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8030..8618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8519..8538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctggcctttt gctcacatgt tcccgcctgc ctcccacagc acgggagtcc
       61 ggtgcttgtt cccaataatg acgtcagtga gcgatgacct cagcgcctgg gaggcgcggg
      121 cgagctggcg ggcggggcgg agaggggcgg gcgcaggcgg aggggagagg cgagacaccg
      181 gaggggcacg cccccatccg ccagcgtcgg ccaatgggcg ccaccgacgg ctccccgcct
      241 ccccgagtgg tatgaaaggc gcagcccggg gaaagtccgg gcagagcccg agaggtggcc
      301 agaccgtcat catcgttcta atacagctac ttcctcagcc accggtatga gccacgggaa
      361 gagaaccgac atgctcccgg agatcgccgc cgccgtgggt ttcctctcca gtctcctgag
      421 gactcggggc tgcgtgagcg agcagagact caaggttttc agtagggcgc tccaggacgc
      481 actgaccggt gagcacacgt cggggtcttg gaaccactcg ggcgcggcca cttcttgcct
      541 ggggtgtctt tatcactgtt ctgtgacggg gacccctacg cgaacaactc cgggtctcag
      601 atgtctttct ctaattaatc tctgaggcca ctgatggtcc tctgtccctt cggttaagtc
      661 cccgtctccc agccctccag tccccgcctc gctcccgagg ctccgaccct cttccttgag
      721 tacattctga gaccctgaca acaccatgag aaaagtcagt gtgtgtgtgt attcagagtc
      781 cgagctcacc cttatctccg aatcttggag aacaacagac cctcagggga tgtgaagcca
      841 ggccaagctg tgcgactttt atgcgcaatt gaactttcta gacaactctt gagtcctagc
      901 cactgttgct gtatagtcct gagcctgtca tcatacaggc ccattctgcc gcgtgtttac
      961 ccgctgcaag ttgtaatctg ggcccccttt gtattttttc ttttttggtc cctcttcacc
     1021 atctgccacc tattgtagta cagaaaagag cctggtagcc tagccaactg cgagggcggg
     1081 gagggggtgt gtgtgttatg gaggcagggt gtgagggacg gaggtggagg agtgataagc
     1141 caccctttag cctctgtccg cagctgttat aactggttgg ttgccttggg ctgcgatagc
     1201 catctctcaa catgttggca cagatctgag gaccaacagc tgtttcatca aaacaaaacc
     1261 attttttatc cccttacctt tgctaaagat ctcaaaactc ttctgatgcg gaagggatga
     1321 gaggccttgt gacttgttgg gcaaccaaca acattggccg cttacctacc ggctttgaaa
     1381 tttcagtgcc ttggtcgtca gaggccctgc cggttcttgc tagcccttcc tgccttaaac
     1441 aggattgtgc caaaaatatc catgaccacc atagagcatc tacctgaaag ctctggggct
     1501 gctttccagt gtacaaaaac ttggaaagga cccaggaatc tttctaagtg gcagggggag
     1561 tttaaggaga ggctcttaaa tactgtcgct gagaagagga agtctcctcc ccagccctct
     1621 cccctcatcc tccccttgtg gtaaacatca gagaatgctt tctaacggcg catcttcctc
     1681 ttgttttttt ccacagatca ttacaaacac cactggtttc cagaaaaacc atccaaaggt
     1741 tctggctatc gctgtatccg tatcaaccac aagatggacc ccatcatcag caaggtggcc
     1801 agccagattg gactcagcca gccccagctg caccggctgc tgcccagtga gctgaccctg
     1861 tgggttgatc cctatgaagt gtcttaccgc atcggggagg atggctccat ctgtgtcctg
     1921 tatgaggagg ccccggtggc tgcctcctat gggctcctca cctgcaagaa ccagatgatg
     1981 ctgggcagga gcagcccctc gaagaactat gtgatggccg tctccagcgg cggatccggg
     2041 ggaagtaaag gtgaagaact gttcaccggt gttgttccga tcctggttga actggatggt
     2101 gatgttaacg gccacaaatt ctctgttcgt ggtgaaggtg aaggtgatgc aaccaacggt
     2161 aaactgaccc tgaaattcat ctgcactacc ggtaaactgc cggttccatg gccgactctg
     2221 gtgactaccc tgacctatgg tgttcagtgt ttttctcgtt acccggatca catgaagcag
     2281 catgatttct tcaaatctgc aatgccggaa ggttatgtac aggagcgcac catttctttc
     2341 aaagacgatg gcacctacaa aacccgtgca gaggttaaat ttgaaggtga tactctggtg
     2401 aaccgtattg aactgaaagg cattgatttc aaagaggacg gcaacatcct gggccacaaa
     2461 ctggaatata acttcaactc ccataacgtt tacatcaccg cagacaaaca gaagaacggt
     2521 atcaaagcta acttcaaaat tcgccataac gttgaagacg gtagcgtaca gctggcggac
     2581 cactaccagc agaacactcc gatcggtgat ggtccggttc tgctgccgga taaccactac
     2641 ctgtcctacc agtctaaact gtccaaagac ccgaacgaaa agcgcgacca catggtgctg
     2701 ctggagttcg ttactgcagc aggtatcacg cacggcatgg atgaactcta caaatgataa
     2761 gccgcgaatt cgcccttcgc gggatcctac ggtacttatt gccaagaaag cacgagcatc
     2821 agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca
     2881 gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc
     2941 aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga
     3001 tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt
     3061 caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact
     3121 tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg
     3181 atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa
     3241 agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc
     3301 tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc
     3361 agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca
     3421 gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc
     3481 aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga
     3541 tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt
     3601 caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact
     3661 tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg
     3721 atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa
     3781 agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc
     3841 tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc
     3901 agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca
     3961 gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc
     4021 aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga
     4081 tctaagggcg aattcgttta taggagccac ccgaccctgg cactctactg ttctcatgct
     4141 gccctgacaa caggccaccg tatacctcaa cctggggaac tgtattttta aaatgaagag
     4201 ctatttatac atgttttttt ttttttttta agaaaagagg aaaaaaacca aaagattttt
     4261 tttaaaaaaa aaagaaaaag aaaaacaatt ctttaaaggg agctgcttgg aagtggcctc
     4321 cccaggtgcc tttggagaga actgttcttg attgcatctg tgagccagtg tttgcctagg
     4381 ggaatgggtt ggggattggc ctagccaagg taaaagggga ttcttggctg atccccccag
     4441 gaggtggtgg aagggagcaa ggttagcaac tgtgaatgag aggggtcagg gtctgctctg
     4501 ggttaccgtc ccagctggga tgcctgtatg cctggtccct ctcttactca ggggcattca
     4561 agcctgatct taaataatac tacattgcct aatcttctct tttgtttttc ggctgagatc
     4621 aggggcagac tgaaaggcct ctcctgtccc ttctgttcta agcagtctct tgaagccgtg
     4681 tctcgtttct gagtctaccc ttgggggcct gaagagcttg cttcccagcc cggaatctgt
     4741 ctaaacattt tttggagggt gggatgtaag gcaggcgcgc aaagactttg gggctaagat
     4801 ggagaggacc tgcacaaaac ctttgctttg ctctgtgctg ctttgtatgg gtggatagtg
     4861 aataatttag ggatgatttg caatggaatt ttgggaccca aagagtatcc aatgggggta
     4921 ggtcttttgg acccagtcct tccttttggg aaccacacga cagtctgaat gctgctacca
     4981 ctattccttt gagaggtggc tcaaagctcc agggaactcc aagtcctttc ttactgcctt
     5041 ctcttcaaga gcaaccttcc ccattttttc tttccccttt cctgcggttg ggtcctggag
     5101 ggccctattt cctaggacaa gcgttctcag tcactgtgca atagtcccag gatgctctga
     5161 gaccggacct cccagcccct cctgatgccc tggtaggttt tagggaccca ttcttcccat
     5221 tcttctaggg gttctgactg gctggtgggc cttgcggaga tcttcctggg ccacagggag
     5281 ggcacctgtg cactgcagga ctacctggta ttcttgtagg gctgccatga agagtcaaac
     5341 cttgggcaca gctttagctc cttggtgctc agagcacctg tgggggaggt tacctctctc
     5401 tctctcttag taaaatccaa atttatttgt agatgtgtgc aatatttact gttctgggtt
     5461 ggagaaaatt gggaaacact gggaagaaat ggctttcctt caggttcagt gacactgatg
     5521 agggcttctt agaaggcctc aagtctctca aactgaagga cagagctaga gctagcccgt
     5581 caccccttgg tgaggattcc cttccccgtt tctctccacc gccatggcat cctgtgtcct
     5641 agatttctca gctcctcagt ttctgctcaa aggtgctatt taccaaactc tctgcctgcc
     5701 cgggcagaca ggccccagct tcgcacagcc ttcccaggtg gcttcgtctc tcttgcttta
     5761 accttaactc tgggcccaca gacctgagag ctgtggccta caacaaagct gtgaattgtt
     5821 ccagatggtt cttgtgtttt gtccgcacac aggtgcctgc cgtttagaag ctgcctcctg
     5881 gtctcatgct taaatcttca attctttact gtccttgttc actttagaaa tgacaaaccc
     5941 tagagctgga ctgttgagca ggcctgtctc tcttattaag taaaaataag aaatagtggt
     6001 aagtttgtaa gctattctga cagaaaagac aaaggttact aattgtataa tagcgctttt
     6061 atatggaaga ctgtacagct ttggcgcctg atgcggtatt ttctccttac gcatctgtgc
     6121 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     6181 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     6241 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6301 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6361 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6421 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6481 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     6541 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6601 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6661 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6721 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6781 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6841 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6961 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7021 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7081 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7141 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7201 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7261 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7681 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8641 tacggttcc
//
