LOCUS       sequence-256623-       10007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Swap out effector and terminator to generate SPARC2-I CRISPR-donor 
            vector for CRISPR-HDR genomic insertion near the attP40 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE,
            Ishida IG, Maimon G, Wilson RI, Clandinin TR
  TITLE     SPARC enables genetic manipulation of precise proportions of cells.
  JOURNAL   Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 
            10.1038/s41593-020-0668-9. Epub 2020 Jul 20.
  PUBMED    32690967
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-0668-9"; journalName: "Nat Neurosci"; date: 
            "2020-09"; volume: "23"; issue: "9"; pages: "1168-1175"
COMMENT     Sequence Label: pHD-SPARC2-I-LexA::p65
FEATURES             Location/Qualifiers
     source          1..10007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2348..2442
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2476..2570
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2604..2698
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2732..2826
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2851..3089
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3036..3057
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          3300..3365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3495..3515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3787..3921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4324..4393
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(4400..4651,4652..5005)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             5450..5821
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     protein_bind    6125..6158
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6384..6393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6390..7067
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6520..6538)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6570..6590)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6788..6807
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6982..7005
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    7188..7309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7316..7349
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9008..9025)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9008..9024)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9123..9140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9294..9882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9374..9393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10006..10007,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg cacagaaaag catcttacgg atggtgtttg
     1261 ccaaaagtgc tggagatgtt ttagatatag atggtttatt ttaagaaatg gatgtatata
     1321 attgagctac tgcatcacgg gtttctgttt aacagtaaca gtgaaaagtg ctcaatgata
     1381 catatattac aaataataac ttttggtttt taaagtagat ttatataata taatcataat
     1441 tcttaatggg aatccatgtt acgaccaaat tagccgtggc ctttcaagtg gctgccactt
     1501 atattttaca actcgcacac agtggctcac accctcgtaa acaaaataat gctattaaat
     1561 caaattgaac atgtgaattc tctcaagaag acgccacttt tttttggggg gccagctgca
     1621 actgccacca atgtttactt ttatctttaa tgggcacatg ccttacatat acgaatttta
     1681 cacgattttt caagtggcgg tcgagagtgg ggtgataatt aattaaggtt gcctttagat
     1741 aaagtattat agagtttcaa aaaaatgaaa cttaagaatg gaatagctgc attccattct
     1801 atgtagattt cccttcaaaa ttattaaaat ccgttgacct ttgttcattt agcaattaga
     1861 attcgtctat gaataacaaa ttaaattgcg ttgcgggcaa attgctttag gccatttgaa
     1921 gtgcaacttt atatctcagg gccaacttct cggcgcgaca acaataaaaa catatacata
     1981 gatacacata tcagatgcaa tgcaatcacc acagcgtgac gtcattagtc taggcttgaa
     2041 aatacacggc aaacttgttt ataactctac gctgattaca taagccgcaa acacgacgaa
     2101 aaaacatttg ttttggtttc aaatccatga atgactttca ttaagtgcag ctgtaagctg
     2161 agctttgcca tgcgctgacc catattttca gcctctcgtt ctctctgttt cgcagatact
     2221 gtgaatatta gtaaaacgat ccataggggc tgtagtttat aatcataatc aaataggcat
     2281 aaacaaccac cagcgcggcc gcggacatat gggatccagt gcaccgtaag cttgcatgcc
     2341 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     2401 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagccctag
     2461 ggcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     2521 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct
     2581 agcgctagcg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     2641 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     2701 gagactctag cactagtgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     2761 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     2821 tccgagcgga gactctagcg acgtcgagcg ccggagtata aatagaggcg cttcgtctac
     2881 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     2941 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     3001 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     3061 taattattga atacaagaag agaactctga attaacgtaa gctagctaga ggatctttgt
     3121 gaaggaacct tacggtacca atcaaaagat ctactccagt ccccaactgg ggtaaccttt
     3181 gagttctctc agttggggta acgtaagcta gctagaggat ctttgtgaag gaaccttact
     3241 tggtaccctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     3301 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     3361 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     3421 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     3481 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     3541 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     3601 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     3661 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     3721 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3781 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3901 gtatcttatc atgtctggat cgatctggcc ggcgggagta gtgccccaac tggggtaacc
     3961 tttgagttct ctcagttggg ggcgtagggt cggggtcggc atggcatctc cacctcctcg
     4021 cggtccgacc tgggcatccg aaggaggacg tcgtccactc ggatggctaa gggagagctc
     4081 ggatccgggc aatacgaggt actggcagac acgcagtgtc gaacaacacc tgacctaacg
     4141 gtaagatgag tagataactc aactacttaa atcctggcct gcttgatatc tcgtagcgtt
     4201 cttagagatc gacgaactgt ttcacgaccg ggtcggcatg gcatctccac ctcctcgcgg
     4261 tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg agagctcgga
     4321 tccctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     4381 cacctcaccc atcgtcgaca tgaaggctct cacggcccga caacaggaag tttttgattt
     4441 gatacgggat catatatccc aaacgggtat gcctccgacc cgcgcagaga tagcacagcg
     4501 actgggcttt cgatcgccta acgccgcgga ggagcacttg aaggcactgg cccgcaaggg
     4561 tgtcattgaa atcgtgtccg gtgcgagccg cggaatccgg ctgttgcagg aggaggaaga
     4621 gggcctgcca ctggtgggac gcgtggccgc tggcgagccg ctgctggccc agcaacacat
     4681 agagggacac tatcaggtgg acccctcctt gtttaagcca aatgctgatt tcctgttgcg
     4741 ggtgtcggga atgtccatga aggacatcgg tattatggat ggtgacctcc tcgccgtcca
     4801 taagacacag gatgtcagga acggccaggt agtcgttgcc aggatagacg atgaggtcac
     4861 tgtgaaacgt ctcaagaagc aaggcaataa ggtcgagctg ctgccggaga atagcgagtt
     4921 caagccgatc gtggtggatc tgcgacagca gtcctttact atcgagggct tggccgtggg
     4981 tgtgatccgc aacggagatt ggctgggatc cacgccgatg gagttccagt acctgcccga
     5041 tacggatgac cgtcaccgta tcgaagaaaa gcggaagcga acctatgaaa ccttcaagtc
     5101 catcatgaaa aagtccccct tctcgggccc cacggacccg cgccccccgc cccgtcgtat
     5161 tgcggttcct tcgcgcagca gtgccagcgt ccccaaaccc gcaccgcagc cctacccgtt
     5221 cacttcctcc cttagcacga ttaactatga tgagttcccc acgatggtgt tccccagtgg
     5281 acaaatttcc caggcatcgg cactggctcc ggccccaccg caagtcctcc cccaggcgcc
     5341 cgctccggca ccggctcccg caatggtgag tgctctggcc caggcccccg ctccagtccc
     5401 cgtgctggcg cctggacccc cacaggcagt tgcccctcct gctccgaaac caacgcaggc
     5461 gggcgaagga accctgagcg aggccctctt gcagcttcag ttcgatgacg aagacttggg
     5521 agccctgctg ggtaacagca cagaccctgc cgtattcacc gatctcgcat ccgtggacaa
     5581 cagcgagttt cagcagctct tgaatcaggg aatcccggtc gcacctcata ccacagagcc
     5641 catgctgatg gaatacccgg aggctatcac gcgactggtg accggcgcac agcgaccacc
     5701 cgatccagcc cctgccccac tgggtgcccc gggtttgccc aatggcctcc tcagcggcga
     5761 tgaggatttc tccagcatcg ctgatatgga tttctccgct ttgctgagcc agataagctc
     5821 ctaagtttaa acaagcttat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt
     5881 cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt
     5941 aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat
     6001 tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca
     6061 gggagtgagt ttgcttaaaa actcgtttag atccactagg tcgactgagg cactgcctcg
     6121 agagataact tcgtataatg tatgctatac gaagttatcg tacgggatct aattcaatta
     6181 gagactaatt caattagagc taattcaatt aggatccaag cttatcgatt tcgaaccctc
     6241 gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     6301 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     6361 aaacaatcgg ctcgaagccg gtcgccacca tggcctcctc cgaggacgtc atcaaggagt
     6421 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     6481 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     6541 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg
     6601 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct
     6661 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     6721 cctccctcca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg aacttcccct
     6781 ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcgtccacc gagcgcctgt
     6841 acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg
     6901 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg
     6961 gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg
     7021 tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagggg ccgcgactct
     7081 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     7141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     7201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ccggtataac
     7321 ttcgtataat gtatgctata cgaagttatc acattgggta gcacagactc tgagctttac
     7381 tacgattact atacagctct tctctcgcga ctttttggac tggacaaggc gtagcacatt
     7441 gaacggcagt gggtttgggt ttagtatcga accggctttc tacgacagcg gattggaagc
     7501 gcggagcgac aaagtcgccg ggtaataatg tgatatagcc ggctatgttc agcaggcaaa
     7561 actgaaataa aagtaataaa caccgaaagc ccccgaattc tcgcatcttc ttttgtggga
     7621 ctgcgagaag aattgcgagc gaaatactaa tttccttacc taactgtgtg agataatctc
     7681 cggttggcat tgtgtgtcgc gcaataaaat ccccaaaagc ccaaagcttc aagtgtttaa
     7741 tataataaaa tagccaagaa gcgtgtgtat ttgcatttgg gaaaaattta aggttgaatt
     7801 acgtgggccg aaaaattaaa gactgaaggc cattcaaggg ttgttcaaag agaaaattcg
     7861 gtgaaaagtc accgtgtgtg tgtgtgtgag tgtgtgtgcg ttgcgtgaga aaaatcaatt
     7921 ctgcatttgg tggaaagccg aaaacggaga gaaaattggt ggagcgagga gaaaaggaca
     7981 cgaattaaag gctggttttt tggggctgcg aaaaactcgt gaccgatgac aatttggctt
     8041 gacgagggtc tattagcgaa atcaatgcta ttatggctga tagtggagga ccaggatcca
     8101 agcacatgga tccaacaacc atagatgccg gcgggggcgg agcaggagca gccggaggtg
     8161 atgacgtgcc tccagtccca gctgttcgcc ggcgtcgtgc gcatgagcag aaatcctcga
     8221 gagagcaggt gctggaggag gagaagagcc aacagctgga gacatcgacg atgacgagga
     8281 cctacatgaa gaccatcacc acctccctca ccacctcgag tagctccaac gtggaagagt
     8341 ttatcctggg ggagcacgca gcaggagcag cagctgctcc atcgcccaac cagcagcgat
     8401 tgcgtcaaaa ggtggcccag tacgagaagg tctggtcgga tggctccagt cccgttaagc
     8461 gtccagccga gcagagctct gatgagctgc gtctgaccga cgaccaggat gagtacgcca
     8521 tccgtaagat gcttttctgt gtcaatcgag ttcaagggcg acacaaaatt tattctaaat
     8581 gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta tcgttgacat
     8641 gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt tattttctta
     8701 attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa tagatgaata
     8761 gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct
     8821 tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac aggcgccctt
     8881 aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg
     8941 gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca gggggcccga
     9001 gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca
     9061 tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc gccttccgct
     9121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg ggctaactac
     9721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9901 tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg
     9961 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgctttt
//
