LOCUS       sequence-256626-        7089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyTag003-Talin rod-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, 
            Hytonen VP, Howarth M
  TITLE     Approaching infinite affinity through engineering of peptide-protein
            interaction.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116.
  PUBMED    31822621
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116."
COMMENT     Sequence Label: SpyTag003-Talin rod-mCherry
FEATURES             Location/Qualifiers
     source          1..7089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..54
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             6379..7089
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6524..6542)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6792..6811
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggccgtg gcgtgcctca tatcgtgatg gtggacgcct acaagcgtta caagcagcag
       61 tacaaccgag tggggaaagt ggagcacggc tctgtggctc tgccagccat catgcgctct
      121 ggagcctctg gtcctgagaa tttccaggtg gggagcatgc cacctgccca gcagcagatc
      181 accagtggcc agatgcaccg aggacacatg ccacctctga cttcagccca gcaggcgctc
      241 actggaacca ttaactccag catgcaggct gtgcaggctg cccaggccac tctggatgac
      301 tttgagactc taccccctct tggccaggat gctgcctcca aagcttggcg taagaacaag
      361 atggatgaat caaagcatga gatccactcc caggtagatg ccatcacagc tggcactgcc
      421 tctgtggtaa acctgacagc aggagatcct gcagagacag actatacagc agtgggctgt
      481 gcagtcacca ccatctcttc caacctgacg gagatgtccc gtggggtgaa gctattggct
      541 gccctgctgg aggatgaagg cggcaatggc cggccccttc tgcaagcagc aaagggcctt
      601 gcaggggctg tgtcggaact gcttcgcagt gctcagcccg ccagcgctga gcctcgtcag
      661 aacctgctgc aggcagccgg gaacgtgggc caggccagtg gggagctgtt gcagcaaatt
      721 ggggaaagtg acactgaccc ccacttccag gatgttctaa tgcagctagc aaacgcagtg
      781 gcaagtgctg cagccgccct ggtcctcaag gccaagagtg tggcccagcg cacagaggat
      841 tccgggcttc agacccaagt gattgctgca gctacgcaat gtgctctgtc cacttcccag
      901 ctggtggcct gtaccaaggt ggtagcacct acaatcagct cacctgtgtg ccaagaacag
      961 ctagtcgagg ctgggcgact ggtggccaaa gctgtggagg gctgtgtgtc tgcctcgcaa
     1021 gcagctacag aagatggaca gctgcttcga ggggtaggag cagcagccac ggctgtcacc
     1081 caggccctca atgagctgct gcagcacgtg aaggcccacg ccaccggagc tgggcctgcc
     1141 ggtcggtatg accaagccac tgataccatc ctcaccgtca ctgaaaacat cttcagctcc
     1201 atgggtgatg ctggggagat ggtgcgccag gcccgcatcc tggcccaagc cacctcagac
     1261 ctggtcaatg ctatcaaggc cgatgctgag ggggagagtg atctggagaa ctctagaaag
     1321 ctcctgagtg ctgccaagat cctcgctgat gccaccgcca agatggtgga ggcggccaag
     1381 ggagcagccg cccaccctga cagtgaggaa cagcagcagc gactgcgtga agcagctgag
     1441 gggcttcgca tggccaccaa tgcagctgcg cagaacgcca tcaagaagaa gttggtgcag
     1501 cgcctggagc atgcagccaa gcaagctgca gcctctgcaa cacagaccat tgctgcagcc
     1561 caacatgcag cctctgcccc caaggcctct gcgggccccc agcccctact ggtgcagagc
     1621 tgtaaggccg tggcagagca gattccgttg ctggtgcagg gtgtccgagg aagtcaagct
     1681 cagcctgaca gccctagtgc tcagctcgcc ctcattgctg ccagccagag cttcctgcag
     1741 ccaggtggga agatggtggc agcggcaaaa gcctcagtgc caacaattca ggaccaggct
     1801 tcagcgatgc agctgagtca gtgcgcaaag aacctaggca ctgccctggc cgagctgcgc
     1861 actgctgctc agaaggctca ggaggcatgt ggacctttgg agatggattc tgcactgagt
     1921 gttgtacaaa atctagagaa agacctacag gaaataaaag cagcagctcg agatggcaag
     1981 cttaagccct tacctgggga aacaatggag aagtgtaccc aagatcttgg caacagcacc
     2041 aaagcggtga gctctgccat cgccaagctg ctgggggaga ttgcccaggg caacgagaac
     2101 tatgcaggta ttgcagctcg ggatgtagct ggtggactaa ggtcactagc ccaggctgcg
     2161 cggggtgtgg ctgcgctgac atcagatcct gcagtgcagg ccattgtgct tgacacagcc
     2221 agcgacgttc tggacaaggc cagcagcctc attgaagagg caaaaaaagc atctggacac
     2281 ccaggggacc cagaaagcca gcaaaggctt gctcaggtag ctaaagcagt gacccaagcc
     2341 ctgaaccgct gtgtcagctg cctgcctggc caaagagacg tggataatgc cctacgagcc
     2401 gttggagatg ccagcaagcg actcctgagt gacttgcttc ctccaagcac ggggacattt
     2461 caagaagcac agagccgatt gaatgaagct gcagctgggt taaatcaggc cgccacagaa
     2521 ctggtgcaag cctcccgagg aactcctcag gacctggctc gggcctccgg ccgatttgga
     2581 caggacttca gcaccttcct ggaagctggt gtggagatgg ccggacaggc cccgagccag
     2641 gaggaccgag cccaggtggt gtccaacctg aagggcatat ccatgtcttc aagcaaactt
     2701 ctccttgccg ctaaggcctt gtccacagac cctgcttctc ccaacctcaa gagtcagctg
     2761 gctgcggctg cccgggcagt gacggacagc atcaaccagc tcatcaccat gtgcacccag
     2821 caggcacctg gccagaagga gtgtgacaat gcacttcggc agctagagac agtccgagaa
     2881 ctcctggaga atccagtcca gcccatcaac gacatgtcct acttcggttg cttggacagt
     2941 gtcatggaga actccaaggt cctaggtgag gccatgactg gcatctccca aaatgccaag
     3001 aatggaaatc tgccggagtt tggagatgcc attgccacag cctccaaggc tctctgtggc
     3061 ttcacggagg cagccgcaca ggcagcatat ctagttggtg tctctgaccc caacagccaa
     3121 gctggacagc aaggactggt ggaacccaca cagtttgccc gtgcaaacca ggcaattcag
     3181 atggcctgtc agagtctggg ggagcctggc tgtacccagg cccaggtgtt atctgcagcc
     3241 actattgtag ccaaacacac atctgcattg tgtaacagct gtcgcctggc ttccgctagg
     3301 actgccaatc ccactgccaa gcgccagttt gtacagtcag ccaaggaggt ggccaacagt
     3361 acagccaatc ttgtcaagac catcaaggca ctagatgggg acttcacaga agagaaccgt
     3421 gcccagtgcc gagcagccac agcccctctg ctggaagctg tggataacct gagtgccttt
     3481 gcctccaacc ctgagttctc cagcgtccct gcccagatca gccctgaggg cagagcagcc
     3541 atggagccca ttgtaatctc tgctaagaca atgttggaga gtgctggagg gctcatccag
     3601 acagctcggg cgttagcagt caatccccga gaccccccac gttggtctgt gctagctggc
     3661 cactctcgga ctgtctcaga ttccatcaag aaacttatta caagcatgag ggacaaagcc
     3721 ccagggcagc tggagtgtga gacagccatt gcggctctga acagctgctt gcgggaccta
     3781 gatcaggctt cgcttgccgc tgtcagccag cagcttgctc cccgtgaagg aatctctcaa
     3841 gaggctttgc acacccagat gctcactgca gtgcaagaaa tttctcatct cattgagccg
     3901 ctggccagcg ctgctcgggc tgaagcctcc cagctgggac acaaggtgtc ccaaatggcc
     3961 cagtactttg aaccactcac cctggctgca gtgggtgctg cgtctaagac cctgagccac
     4021 ccgcagcaga tggcacttct ggaccagact aaaacgttgg cagagtctgc cttgcagttg
     4081 ctatatactg ccaaggaggc tggtggtaac cccaagcaag cagcacacac ccaggaagcc
     4141 ctggaggagg ctgtgcagat gatgacagag gccgtagaag acctgacgac gaccctcaac
     4201 gaagcagcca gtgctgcagg agtcgttggc ggcatggtgg actctatcac tcaggccatc
     4261 aaccagctag atgaaggacc tatgggtgac ccagaaggct cattcgtaga ttaccagaca
     4321 accatggtga ggacagccaa ggccattgct gtcactgttc aggagatggt aaccaagtca
     4381 aacaccagcc ctgaagagct aggccctctt gccaaccagc tgaccagtga ctatggccga
     4441 ctggcctcac aagccaagcc tgcagctgtg gctgctgaaa atgaagagat aggcgctcac
     4501 atcaaacacc gagtacagga gctgggccat ggctgctctg ctctggtcac caaggcaggt
     4561 gccttgcagt gtagccccag tgatgtctac accaagaagg agctcataga gtgtgcccgc
     4621 agagtgtcag agaaggtctc ccatgttctg gctgcactcc aggctgggaa tcgtggtacc
     4681 caggcctgca ttacagcagc cagtgctgtg tctggtatca ttgctgacct cgacaccacc
     4741 atcatgttcg ctactgctgg cacacttaac cgtgagggtg ctgaaacttt tgctgaccac
     4801 cgggagggta tcttaaagac agcaaaggtt cttgtggagg acaccaaggt cctagtgcag
     4861 aatgcagctg ggagccagga gaagttggca caagccgccc agtcctccgt ggccaccatt
     4921 acccgcctcg ctgatgtggt caagctcggt gcagccagcc taggagccga agaccctgaa
     4981 actcaggtgg tgctgatcaa tgcagtaaag gacgtagcca aggccctggg tgacctcatc
     5041 agcgctacga aggctgcagc gggcaaagtt ggggatgacc ctgcagtgtg gcagctcaag
     5101 aactctgcca aggtgatggt gaccaatgtg acatcattgc tcaagacagt gaaggctgtg
     5161 gaagatgagg ccaccaaagg cacacgggcc ctagaggcaa ccacagagca catacgtcag
     5221 gaactggcgg tcttctgttc cccagagcca cctgccaaga cctctacccc tgaagacttc
     5281 atccgaatga ccaagggtat tactatggca acagccaaag ccgttgctgc tggcaattcc
     5341 tgtcgacagg aagatgtcat tgccacagcc aatctgagcc gacgggctat tgcggacatg
     5401 cttcgggctt gcaaggaagc agctttccac ccagaagtgg cgcctgatgt acggctccga
     5461 gccctgcact atggccggga gtgtgccaat ggttacctgg aactgctgga ccacgtgctg
     5521 ctgacccttc agaagccaaa cccagacctg aagcagcagc taactggaca ctcaaagcgg
     5581 gttgctggct ctgtgactga gctcatccag gctgctgaag ccatgaaagg aacagagtgg
     5641 gtggacccag aggaccctac tgtcattgct gagaatgagc tcttgggagc cgcagccgcc
     5701 atcgaggctg cagccaagaa gctggagcag ctgaagccca gggccaaacc caaggaggcg
     5761 gatgagtcct tgaactttga ggaacaaatc ctagaagctg ccaagtccat cgctgcagcc
     5821 accagtgcac tggtaaaggc tgcgtcagca gcccagaggg agctggtggc tcaaggaaag
     5881 gtgggcgcca ttccagccaa tgcactggat gatgggcagt ggtcgcaggg cctcatttcc
     5941 gctgcccgta tggtggctgc ggccaccaac aatctgtgtg aagcagctaa cgcagctgtc
     6001 cagggccatg ctagccagga gaaactcatt tcctcagcca agcaagtagc tgcctccaca
     6061 gctcagctcc tggtagcttg caaggtcaaa gcagatcagg actctgaggc aatgaaacgg
     6121 ctccaggctg ctggcaatgc agtgaagagg gcctcagata acctggtaaa ggcggcccag
     6181 aaagctgcag ccttcgaaga ccaggagaat gagacggtgg tggtgaagga gaaaatggtt
     6241 gggggcattg cccagattat cgcagcacag gaagagatgc ttcggaagga acgagagctg
     6301 gaagaggctc ggaaaaagct cgcccagatc cggcagcagc agtacaagtt cttgccttca
     6361 gagcttcgag acgagcacat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     6421 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     6481 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     6541 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     6601 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     6661 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     6721 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     6781 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     6841 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     6901 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     6961 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     7021 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     7081 tacaagtaa
//
