LOCUS       sequence-25663-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycQKI
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             121..150
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg gactctagcc taggccgcgg taccaccatg
      121 gaacaaaaac ttatttctga agaagatctg tctagatcaa caagtttgta caaaaaagca
      181 ggctccacca tgggaaccaa ttcagtcgac atggtcgggg aaatggaaac gaaggagaag
      241 ccgaagccca ccccagatta cctgatgcag ctgatgaacg acaagaagct catgagcagc
      301 ctgcccaact tctgcgggat cttcaaccac ctcgagcggc tgctggacga agaaattagc
      361 agagtacgga aagacatgta caatgacaca ttaaatggca gtacagagaa aaggagtgca
      421 gaattgcctg atgctgtggg acctattgtt cagttacaag agaaacttta tgtgcctgta
      481 aaagaatacc cagattttaa ttttgttggg agaatccttg gacctagagg acttacagcc
      541 aaacaacttg aagcagaaac cggatgtaaa atcatggtcc gaggcaaagg ctcaatgagg
      601 gataaaaaaa aggaggagca aaatagaggc aagcccaatt gggagcatct aaatgaagat
      661 ttacatgta
//
