LOCUS       sequence-256639-        8238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-bpA-F-L-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2253..2315
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2620..2999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3001..3278
                     /label=chicken beta-actin promoter
     intron          3279..4296
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4220..4242
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4304..4323
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4356..4389)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    4419..4643
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4709..4742)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(4755..4788)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4798..4800,4801..5511)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4798..5511
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(5542..5544,5545..6258)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5542..6255
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6316..6336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6734..6745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6897..7121
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7475..7509
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7596..7615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7597..7615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7622..7639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7622..7638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7631..7653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7780..8235)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7917..7938)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8129..8148
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg acagcaggct
     2221 gaataataaa aaaattagaa actattattt aaccctagaa agataatcat attgtgacgt
     2281 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     2341 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     2401 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     2461 aatttcttct ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta
     2521 ttttgtactt taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac
     2581 ataatagaaa caaattatac ttattaaccg gtcatggtcg acattgatta ttgactagtt
     2641 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2701 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2761 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2821 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2881 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2941 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3001 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     3061 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     3121 ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg
     3181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     3241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc
     3301 cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     3481 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     3541 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3601 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     3661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3721 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     3781 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3901 gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3961 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     4021 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     4081 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     4141 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     4201 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     4261 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     4321 gtgctgtctc atcattttgg caaagaattg ctagcgaagt tcctatactt tctagagaat
     4381 aggaacttcc ccggctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     4441 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4501 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4561 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4621 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgactag
     4681 agcttgcgga acccttctaa ctagataaga agttcctata ctttctagag aataggaact
     4741 tcatcgatac tagcataact tcgtatagca tacattatac gaagttatag gcgcgccatg
     4801 agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     4861 gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa
     4921 ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc aacacttgtc
     4981 actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat gaaacggcat
     5041 gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa
     5101 gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     5161 cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg acacaaactc
     5221 gaatacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc
     5281 aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact agcagaccat
     5341 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     5401 tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
     5461 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa aggttctgct
     5521 ggatctgcag caggatctgg tatgagcaaa ggagaagaac ttttcactgg agttgtccca
     5581 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtccg tggagagggt
     5641 gaaggtgatg ctacaaacgg aaaactcacc cttaaattta tttgcactac tggaaaacta
     5701 cctgttccgt ggccaacact tgtcactact ctgacctatg gtgttcaatg cttttcccgt
     5761 tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     5821 caggaacgca ctatatcttt caaagatgac gggacctaca agacgcgtgc tgaagtcaag
     5881 tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtattgattt taaagaagat
     5941 ggaaacattc ttggacacaa actcgaatac aactttaact cacacaatgt atacatcacg
     6001 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cgttgaagat
     6061 ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     6121 cttttaccag acaaccatta cctgtcgaca caatctgtcc tttcgaaaga tcccaacgaa
     6181 aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg
     6241 gatgagctct acaaatgatt aattaaaacc tctggattac aaaatttgtg aaagattgac
     6301 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6361 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6421 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6481 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6541 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6661 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6721 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6781 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6841 cgcctccccg caggatccac tagagcggcc gcctagagct cgctgatcag cctcgactgt
     6901 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6961 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7021 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7081 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7141 cagctggggc tcgactagag cttgcggaac ccttaagctt ttttattata tattagtcac
     7201 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
     7261 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
     7321 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
     7381 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
     7441 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
     7501 tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa
     7561 tcttcgtttg acaaaaacca cattgtgttg ggtcgcccta tagtgagtcg tattacaatt
     7621 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     7681 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     7741 gcccttccca acagttgcgc agcctgaatg gcgaatggca aattgtaagc gttaatattt
     7801 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7861 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7921 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7981 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8041 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8101 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8161 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8221 cgctacaggg cgcgtcag
//
