LOCUS       sequence-25664-e         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycEIF2C1
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             121..150
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg gactctagcc taggccgcgg taccaccatg
      121 gaacaaaaac ttatttctga agaagatctg tctagatcaa caagtttgta caaaaaagca
      181 ggctccacca tggaagcggg accctcggga gcagctgcgg gcgcttacct gccccccctg
      241 cagcaggtgt tccaggcacc tcgccggcct ggcattggca ctgtggggaa accaatcaag
      301 ctcctggcca attactttga ggtggacatc cctaagatcg acgtgtacca ctacgaggtg
      361 gacatcaagc cggataagtg tccccgtaga gtcaaccggg aagtggtgga atacatggtc
      421 cagcatttca agcctcagat ctttggtgat cgcaagcctg tgtatgatgg aaagaagaac
      481 atttacactg tcacagcact gcccattggc aacgaacggg tcgactttga ggtgacaatc
      541 cctggggaag ggaaggatcg aatctttaag gtctccatca agtggctag
//
