LOCUS       sequence-25665-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycEIF2C2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             122..151
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     gap             576..589
                     /estimated_length=14
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctagc ctaggccgcg gtaccaccat
      121 ggaacaaaaa cttatttctg aagaagatct gtctagatca acaagtttgt acaaaaaagc
      181 aggctccacc atgggaacca attcagtcga catgtactcg ggagccggct cccgagtaca
      241 tgtcgacgcg tgggccggcc cacgcgtcga ctggatccgg atgtactcgg gagccggccc
      301 cgcacttgca cctcctgcgc cgccgccccc catccaagga tatgccttca agcctccacc
      361 tagacccgac tttgggacct ccgggagaac aatcaaatta caggccaatt tcttcgaaat
      421 ggacatcccc aaaattgaca tctatcatta tgaattggat atcaagccag agaagtgccc
      481 gaggagagtt aacagggaaa tcgtggaaca catggtccag cactttaaaa cacagatctt
      541 tggggatcgg aagcccgtgt ttgacggcag gaanannnnn nnnnnnnnnc ccccnnnnnn
      601 ntngganggn acaaggtgga gctggaggtc acgctgccag gagaaggcaa ggatcgcatc
      661 ttcnangnnn nncatcaagt gggtgtcctg cgtggannnn nnnnnggcgt tacacgatgc
      721 actttcaggg cggnctgccc agcgtccctt ttgagacgat ccaggccctg
//
