LOCUS       sequence-256652-       15116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ORFeus expression, WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 15116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA007
FEATURES             Location/Qualifiers
     source          1..15116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5279..5302
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5303..5368
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5578..5712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5855..5877)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6437..8226
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(8528..10453)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        10974..11078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11079..11147,11148..11939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11297..11316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12110..12698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12599..12618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12852..12869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12950..12969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12988..13008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12993..13322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13173..13308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13415..14014
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13415..13434)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13911..13931
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    14244..14378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14443..14462)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14487..14535
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag cggccgcgtt taaacttaat taagccacca tgggcaagaa
      361 gcagaaccgc aagaccggca acagcaagac ccagagcgcc agcccccccc ccaaggagcg
      421 cagcagcagc cccgccaccg agcagagctg gatggagaac gacttcgacg agctgcgcga
      481 ggagggcttc cgccgcagca actacagcga gctgcgcgag gacatccaga ccaagggcaa
      541 ggaggtggag aacttcgaga agaacctgga ggagtgcatc acccgcatca ccaacaccga
      601 gaagtgcctg aaggagctga tggagctgaa gaccaaggcc cgcgagctgc gcgaggagtg
      661 ccgcagcctg cgcagccgct gcgaccaatt ggaggagcgc gtgagcgcca tggaggacga
      721 gatgaacgag atgaagcgcg agggcaagtt ccgcgagaag cgcatcaagc gcaacgagca
      781 gagcctgcag gagatctggg actacgtgaa gcgccccaac ctgcgcctga tcggcgtgcc
      841 cgagagcgac gtggagaacg gcaccaagct ggagaacacc ctgcaggaca tcatccagga
      901 gaacttcccc aacctggccc gccaggccaa cgtgcagatc caggagatcc agcgcacccc
      961 ccagcgctac agcagccgcc gcgccacccc ccgccacatc atcgtgcgct tcaccaaggt
     1021 ggagatgaag gagaagatgc tgcgcgccgc ccgcgagaag ggccgcgtga ccctgaaggg
     1081 caagcccatc cgtctcaccg ccgacctgag cgccgagacc ctgcaggccc gccgcgagtg
     1141 gggccccatc ttcaacatcc tgaaggagaa gaacttccag ccccgcatca gctaccccgc
     1201 caagctgagc ttcatcagcg agggcgagat caagtacttc atcgacaagc agatgctgcg
     1261 cgacttcgtg accacccgcc ccgccctgaa ggagctgctg aaggaggccc tgaacatgga
     1321 gcgcaacaac cgctaccagc ccctgcagaa ccacgccaag atgtaaagac catcgagact
     1381 aggaagaaac tgcatcaact aatgagcaaa atcaggcgcg cccatcataa tgaccggcag
     1441 caacagccac atcaccatcc tgaccctgaa catcaacggc ctgaacagcg ccatcaagcg
     1501 ccaccgcctg gccagctgga tcaagagcca ggaccccagc gtgtgctgca tccaggagac
     1561 ccacctgacc tgccgcgaca cccaccgcct gaagatcaag ggctggcgca agatctacca
     1621 ggccaacggc aagcagaaga aggccggcgt ggccatcctg gtgagcgaca agaccgactt
     1681 caagcccacc aagatcaagc gcgacaagga gggccactac atcatggtga agggcagcat
     1741 ccagcaggag gagctgacca tcctgaacat ctacgccccc aacaccggtg ccccccgctt
     1801 catcaagcag gtgctgagcg acctgcagcg cgacctggac agccacaccc tgatcatggg
     1861 cgacttcaac acccccctga gcaccctgga ccgcagcacc cgccagaagg tgaacaagga
     1921 cacccaggag ctgaacagcg ccctgcacca ggccgacctg atcgacatct accgcaccct
     1981 gcaccccaag agcaccgagt acaccttctt cagcgccccc caccacacct acagcaagat
     2041 cgaccacatc gtgggcagca aggccctgct gagcaagtgc aagcgcaccg agatcatcac
     2101 caactacctg agcgaccaca gcgccatcaa gctggagctg cgcatcaaga acctgaccca
     2161 gagccgcagc accacctgga agctgaacaa cctgctgctg aacgactact gggtgcacaa
     2221 cgagatgaag gccgagatca agatgttctt cgaaaccaac gagaacaagg acaccaccta
     2281 ccagaacctg tgggacgcct tcaaggccgt gtgccgcggc aagttcatcg ccctgaacgc
     2341 ctacaagcgc aagcaggagc gcagcaagat cgacaccctg accagccagc tgaaggagct
     2401 ggagaagcag gagcagaccc acagcaaggc cagccgccgc caggagatca ccaagatccg
     2461 cgccgagctg aaggagatcg agacccagaa gaccctgcag aagatcaacg agtcgcgaag
     2521 ctggttcttc gagcgcatca acaagatcga ccgccccctg gcccgcctga tcaagaagaa
     2581 gcgcgagaag aaccagatcg acaccatcaa gaacgacaag ggcgacatca ccaccgaccc
     2641 caccgagatc cagaccacca tccgcgagta ctacaagcac ctgtacgcca acaagctgga
     2701 gaacctggag gagatggaca ccttcctgga cacctacacc ctgccccgcc tgaaccagga
     2761 ggaggtggag agcctgaacc gccccatcac cggcagcgag atcgtggcca tcatcaacag
     2821 cctgcccacc aagaagagcc ccggccccga cggcttcacc gccgagttct accagcgcta
     2881 caaggaggag ctggtgccct tcctgctgaa gctgttccaa agcatcgaga aggagggcat
     2941 cctgcccaac agcttctacg aggccagcat catcctgatc cccaagcccg gccgcgacac
     3001 caccaagaag gagaacttcc gccccatcag cctgatgaac atcgacgcca agatcctgaa
     3061 caagatcctg gccaaccgca tccagcagca catcaagaag ctgatccacc acgaccaggt
     3121 gggcttcatc cccgggatgc agggctggtt caacatccgc aagagcatca acgtgatcca
     3181 gcacatcaac cgcgccaagg acaagaacca catgatcatc agcatcgacg ccgagaaggc
     3241 cttcgacaag atccagcagc ccttcatgct gaagaccctg aacaagctgg gcatcgacgg
     3301 cacctacttc aagatcatcc gcgccatcta cgacaagccc accgccaaca tcatcctgaa
     3361 cggccagaag ctggaggcct tccccctgaa gaccggcacg cgtcagggct gccccctgag
     3421 ccccctgctg ttcaacatcg tgctggaggt gctggcccgc gccatccgcc aggagaagga
     3481 gatcaagggc atccagctgg gcaaggagga ggtgaagctg agcctgttcg ccgacgacat
     3541 gatcgtgtac ctggagaacc ccatcgtgag cgcccagaac ctgctgaagc tgatcagcaa
     3601 cttcagcaag gtgagcggct acaagatcaa cgtgcagaag agccaggcct tcctgtacac
     3661 caacaaccgc cagaccgaga gccagatcat gggcgagctg cccttcacca tcgctagcaa
     3721 gcgcatcaag tacctgggca tccagctgac ccgcgacgtg aaggacctgt tcaaggagaa
     3781 ctacaagccc ctgctgaagg agatcaagga ggagaccaac aagtggaaga acatcccctg
     3841 cagctgggtg ggccgcatca acatcgtgaa gatggccatc ctgcccaagg tgatctaccg
     3901 cttcaacgcc atccccatca agctgcccat gaccttcttc accgagctgg agaagaccac
     3961 cctgaagttc atctggaacc agaagcgcgc ccgcatcgcc aagagcatcc tgagccagaa
     4021 gaacaaggcc ggcggcatca ccctgcccga cttcaagctg tactacaagg ccaccgtgac
     4081 caagaccgcc tggtactggt accagaaccg cgatatcgac cagtggaacc gcaccgagcc
     4141 cagcgagatc atgccccaca tctacaacta cctgatcttc gacaagcccg agaagaacaa
     4201 gcagtggggc aaggacagcc tgttcaacaa gtggtgctgg gagaactggc tggccatctg
     4261 ccgcaagctg aagctggacc ccttcctgac cccctacacc aagatcaaca gccgctggat
     4321 caaggacctg aacgtgaagc ccaagaccat caagaccctg gaggagaacc tgggcatcac
     4381 catccaggac atcggcgtgg gcaaggactt catgagcaag acccccaagg ccatggccac
     4441 caaggacaag atcgacaagt gggacctgat caagctgaag agcttctgca ccgccaagga
     4501 gaccaccatc cgcgtgaacc gccagcccac cacctgggag aagatcttcg ccacctacag
     4561 cagcgacaag ggcctgatca gccgcatcta caacgagctg aagcagatct acaagaagaa
     4621 gaccaacaac cccatcaaga agtgggccaa ggacatgaac cgccacttca gcaaggagga
     4681 catctacgcc gccaagaagc atatgaagaa gtgcagcagc agcctggcca tccgcgagat
     4741 gcagatcaag accaccatgc gctaccacct gacccccgtg cgcatggcca tcatcaagaa
     4801 gagcggcaac aaccgctgct ggcgcggctg cggcgagatc ggcaccctgc tgcactgctg
     4861 gtgggactgc aagctggtgc agcccctgtg gaagagcgtg tggcgcttcc tgcgcgacct
     4921 ggagctggag atccccttcg accccgccat ccccctgctg ggcatctacc ccaacgagta
     4981 caagagctgc tgctacaagg acacctgcac ccgcatgttc atcgccgccc tgttcaccat
     5041 cgccaagacc tggaaccagc ccaagtgccc caccatgatc gattggatca agaagatgtg
     5101 gcacatctac accatggagt actacgccgc catcaagaac gacgagttca tcagcttcgt
     5161 gggcacctgg atgaagctgg agaccatcat cctgagcaag ctgagccagg agcagaagac
     5221 caagcaccgc atcttcagcc tgatcggcgg caacggcggc ggcagcggcg gcggcagcct
     5281 ggaagttctg ttccaggggc ccgactacaa agaccatgac ggtgattata aagatcatga
     5341 catcgattac aaggatgacg atgacaagta gacaatgaga tcacatggac acaggaaggg
     5401 gaatatcaca ctctggggac tgtggtgggg tcgggggagg ggggagggat agcattggga
     5461 gatataccta atgctagatg acacattagt gggtgcagcg caccagcatg gcacatgtat
     5521 acatatgtaa ctaacctgca caatgtgcac atgtacccta aaacttagag tataatggat
     5581 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5641 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5701 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     5761 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     5821 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5881 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5941 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     6001 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     6061 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     6121 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     6181 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     6241 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     6301 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     6361 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     6421 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     6481 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     6541 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     6601 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     6661 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     6721 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     6781 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     6841 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     6901 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     6961 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     7021 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     7081 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     7141 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     7201 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     7261 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     7321 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     7381 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     7441 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     7501 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     7561 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     7621 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     7681 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     7741 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     7801 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     7861 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7921 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7981 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     8041 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     8101 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     8161 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     8221 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     8281 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     8341 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     8401 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     8461 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     8521 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     8581 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     8641 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     8701 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     8761 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     8821 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     8881 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     8941 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     9001 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     9061 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     9121 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     9181 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     9241 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     9301 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     9361 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     9421 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     9481 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     9541 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     9601 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     9661 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     9721 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     9781 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     9841 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     9901 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     9961 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
    10021 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
    10081 ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
    10141 ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg
    10201 tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc
    10261 ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc
    10321 ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc
    10381 cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc
    10441 cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta gacgacgctc
    10501 agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct
    10561 tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg
    10621 tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg
    10681 tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat
    10741 ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg
    10801 gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca
    10861 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    10921 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    11041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    11101 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    11161 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    11221 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    11281 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
    11341 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    11401 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    11461 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    11521 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    11581 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
    11641 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    11701 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    11761 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    11821 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    11881 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11941 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    12001 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    12061 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    12121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    12181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    12241 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    12421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    12481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12721 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12781 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    12901 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    12961 ctgatgccgc atagttaagc cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    13021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    13081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    13141 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    13201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    13261 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    13321 aaaagcttgc atgcctgcag gtcggccgcc acgaccggtg ccgccaccat cccctgaccc
    13381 acgcccctga cccctcacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg
    13441 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
    13501 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
    13561 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    13621 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    13681 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    13741 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    13801 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    13861 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    13921 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    13981 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
    14041 gaccgaaagg agcgcacgac cccatggctc cgaccgaagc cacccggggc ggccccgccg
    14101 accccgcacc cgcccccgag gcccaccgac tctagaggat cataatcagc cataccacat
    14161 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    14221 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    14281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    14341 tgtccaaact catcaatgta tcttatcatg tctggatcac tcgccgatag tggaaaccga
    14401 cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac
    14461 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
    14521 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
    14581 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
    14641 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
    14701 ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg
    14761 ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc
    14821 atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg
    14881 tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca
    14941 agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt
    15001 gccattgctg caggcgcaga actggtaggt atggaagatc tctagaagct gggtaccagc
    15061 tgctagcaag cttgctagcg gccgaggcgt atcacgaggc cctttcgtct tcactc
//
