LOCUS       sequence-256659-        9395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB542
FEATURES             Location/Qualifiers
     source          1..9395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..32
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             234..263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(431..1513)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1514..1591)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1661..1677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1694..1710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1729..1740
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4927..5013
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    5062..5078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5095..5111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5130..5141
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5193..5246
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with 
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
     terminator      7151..7245
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      7261..7347
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7382..7399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7516..8061)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7596..7615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8175..8269)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8313..9104)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacgtctgtg tggaattgtg agcggataac aaattcacac agggccctcg gacaccgagg
       61 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
      121 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
      181 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
      241 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
      301 aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct tctaacagaa
      361 atgtcctgag caatcaccta tgaactgtcg actcgacaat tcgcgctaac ttacattaat
      421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      541 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      601 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
      661 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      721 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      781 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      841 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      901 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      961 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     1021 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     1081 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     1141 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     1201 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     1261 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     1321 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1381 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1441 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1501 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1561 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gcatgccgct tcgccttcgc
     1621 gcgcgaattg tcgagaaaat ttatcaaaaa gagtgttgac ttgtgagcgg ataacaatga
     1681 tacttagatt caattgtgag cggataacaa tttcacacag aattcattaa agaggagaaa
     1741 ggatccatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg tgtagcgatt
     1801 atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac cacgccttct
     1861 atcattgcct atacccagga tggtgaaact ctagttggtc agccggctaa acgtcaggca
     1921 gtgacgaacc cgcaaaacac tctgtttgcg attaaacgcc tgattggtcg ccgcttccag
     1981 gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc tgctgataac
     2041 ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat ttctgctgaa
     2101 gtgctgaaaa aaatgaagaa aaccgctgaa gattacctgg gtgaaccggt aactgaagct
     2161 gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc
     2221 cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct
     2281 tacggtctgg acaaaggcac tggcaaccgt actatcgcgg tttatgacct gggtggtggt
     2341 actttcgata tttctattat cgaaatcgac gaagttgacg gcgaaaaaac cttcgaagtt
     2401 ctggcaacca acggtgatac ccacctgggg ggtgaagact tcgacagccg tctgatcaac
     2461 tatctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga tccgctggca
     2521 atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag
     2581 accgacgtta acctgccata catcactgca gacgcgaccg gtccgaaaca catgaacatc
     2641 aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc tggtaaaccg ttccattgag
     2701 ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga cgacgttatc
     2761 ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga gttctttggt
     2821 aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc tgctgttcag
     2881 ggtggtgttc tgactggtga cgtaaaagac gtactgctgc tggacgttac cccgctgtct
     2941 ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa caccactatc
     3001 ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc ggtaaccatc
     3061 catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg tcagttcaac
     3121 ctagatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac cttcgatatc
     3181 gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa agagcagaag
     3241 atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac
     3301 gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac tcgcaaccag
     3361 ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga caaactgccg
     3421 gctgacgaca aaactgctat cgagtctgcg ctgactgcac tggaaactgc tctgaaaggt
     3481 gaagacaaag ccgctatcga agcgaaaatg caggaactgg cacaggtttc ccagaaactg
     3541 atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga tgcttctgca
     3601 aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa
     3661 taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt cctctccgcc
     3721 cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt acgagatttt
     3781 aggcgtttcc aaaacagcgg aagagcgtga aatcagaaag gcctacaaac gcctggccat
     3841 gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat ttaaagagat
     3901 caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg
     3961 tcatgctgcg tttgagcaag gtggcatggg cggcggcggt tttggcggcg gcgcagactt
     4021 cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg
     4081 tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag aagctgtacg
     4141 tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt gccacggtag
     4201 cggtgcaaaa ccaggtacac agccgcagac ttgtccgacc tgtcatggtt ctggtcaggt
     4261 gcagatgcgc cagggattct tcgctgtaca gcagacctgt ccacactgtc agggccgcgg
     4321 tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa
     4381 aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc gtcttgcggg
     4441 cgaaggtgaa gcgggcgagc atggcgcacc ggcaggcgat ctgtacgttc aggttcaggt
     4501 taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag tcccgatcaa
     4561 cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg gtcgcgtcaa
     4621 actgaaagtg cctggcgaaa cccagaccgg taagctattc cgtatgcgcg gtaaaggcgt
     4681 caagtctgtc cgcggtggcg cacagggtga tttgctgtgc cgcgttgtcg tcgaaacacc
     4741 ggtaggcctg aacgaaaggc agaaacagct gctgcaagag ctgcaagaaa gcttcggtgg
     4801 cccaaccggc gagcacaaca gcccgcgctc aaagagcttc tttgatggtg tgaagaagtt
     4861 ttttgacgac ctgacccgct aagatgtcga cctgcagcca agcttaatta gctgatctag
     4921 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4981 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta gtcgagaaaa
     5041 tttatcaaaa agagtgttga cttgtgagcg gataacaatg atacttagat tcaattgtga
     5101 gcggataaca atttcacaca gaattcatta aagaggagaa attaaccatg aatattcgtc
     5161 cattgcatga tcgcgtgatc gtcaagcgta aagaagttga aactaaatct gctggcggca
     5221 tcgttctgac cggctctgca gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca
     5281 atggccgtat ccttgaaaat ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg
     5341 ttattttcaa cgatggctac ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga
     5401 tcatgtccga aagcgacatt ctggcaattg ttgaagcgta atccgcgcac gacactgaac
     5461 atacgaattt aaggaataaa gataatggca gctaaagacg taaaattcgg taacgacgct
     5521 cgtgtgaaaa tgctgcgcgg cgtaaacgta ctggcagatg cagtgaaagt taccctcggt
     5581 ccaaaaggcc gtaacgtagt tctggataaa tctttcggtg caccgaccat caccaaagat
     5641 ggtgtttccg ttgctcgtga aatcgaactg gaagacaagt tcgaaaatat gggtgcgcag
     5701 atggtgaaag aagttgcctc taaagcaaac gacgctgcag gcgacggtac caccactgca
     5761 accgtactgg ctcaggctat catcactgaa ggtctgaaag ctgttgctgc gggcatgaac
     5821 ccgatggacc tgaaacgtgg tatcgacaaa gcggttaccg ctgcagttga agaactgaaa
     5881 gcgctgtccg taccatgctc tgactctaaa gcgattgctc aggttggtac catctccgct
     5941 aactccgacg aaaccgtagg taaactgatc gctgaagcga tggacaaagt cggtaaagaa
     6001 ggcgttatca ccgttgaaga cggtaccggt ctgcaggacg aactggacgt ggttgaaggt
     6061 atgcagttcg accgtggcta cctgtctcct tacttcatca acaagccgga aactggcgca
     6121 gtagaactgg aaagcccgtt catcctgctg gctgacaaga aaatctccaa catccgcgaa
     6181 atgctgccgg ttctggaagc tgttgccaaa gcaggcaaac cgctgctgat catcgctgaa
     6241 gatgtagaag gcgaagcgct ggcaactctg gttgttaaca ccatgcgtgg catcgtgaaa
     6301 gtcgctgcgg ttaaagcacc gggcttcggc gatcgtcgta aagctatgct gcaggatatc
     6361 gcaaccctga ctggcggtac cgtgatctct gaagagatcg gtatggagct ggaaaaagca
     6421 accctggaag acctgggtca ggctaaacgt gttgtgatca acaaagacac caccactatc
     6481 atcgatggcg tgggtgaaga agctgcaatc cagggccgtg ttgctcagat ccgtcagcag
     6541 attgaagaag caacttctga ctacgaccgt gaaaaactgc aggaacgcgt agcgaaactg
     6601 gcaggcggcg ttgcagttat caaagtgggt gctgctaccg aagttgaaat gaaagagaaa
     6661 aaagcacgcg ttgaagatgc cctgcacgcg acccgtgctg cggtagaaga aggcgtggtt
     6721 gctggtggtg gtgttgcgct gatccgcgta gcgtctaaac tggctgacct gcgtggtcag
     6781 aacgaagacc agaacgtggg tatcaaagtt gcactgcgtg caatggaagc tccgctgcgt
     6841 cagatcgtat tgaactgcgg cgaagaaccg tctgttgttg ctaacaccgt taaaggcggc
     6901 gacggcaact acggttacaa cgcagcaacc gaagaatacg gcaacatgat cgacatgggt
     6961 atcctggatc caaccaaagt aactcgttct gctctgcagt acgcagcttc tgtggctggc
     7021 ctgatgatca ccaccgaatg catggttacc gacctgccga aaaacgatgc agctgactta
     7081 ggcgctgctg gcggtatggg cggcatgggt ggcatgggcg gcatgatgta agcttaatta
     7141 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     7201 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagaggcat
     7261 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     7321 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggata tattccgctt
     7381 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     7441 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     7501 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     7561 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7621 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     7681 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     7741 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     7801 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     7861 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     7921 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     7981 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     8041 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     8101 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     8161 ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     8221 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     8281 gatatccgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     8341 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     8401 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     8461 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     8521 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     8581 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     8641 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     8701 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     8761 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     8821 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     8881 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     8941 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     9001 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     9061 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt
     9121 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     9181 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
     9241 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     9301 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct
     9361 tatgtccact gggttcgtgc cttcatccgg atatc
//
