LOCUS       sequence-256662-       13620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ORFeus expression, WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA056
FEATURES             Location/Qualifiers
     source          1..13620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..379
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5598..5732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5622..5641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5676..5695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5875..5897)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6457..8246
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10287..10305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10373..10477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10478..10546,10547..11338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10696..10715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11509..12097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11998..12017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12528..12673
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             12716..13114
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    13156..13204
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
       61 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
      121 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
      181 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
      241 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
      361 cacttcctac cctcgtaaag cggccgcgtt taaacttaat taagccacca tgggcaagaa
      421 gcagaaccgc aagaccggca acagcaagac ccagagcgcc agcccccccc ccaaggagcg
      481 cagcagcagc cccgccaccg agcagagctg gatggagaac gacttcgacg agctgcgcga
      541 ggagggcttc cgccgcagca actacagcga gctgcgcgag gacatccaga ccaagggcaa
      601 ggaggtggag aacttcgaga agaacctgga ggagtgcatc acccgcatca ccaacaccga
      661 gaagtgcctg aaggagctga tggagctgaa gaccaaggcc cgcgagctgc gcgaggagtg
      721 ccgcagcctg cgcagccgct gcgaccaatt ggaggagcgc gtgagcgcca tggaggacga
      781 gatgaacgag atgaagcgcg agggcaagtt ccgcgagaag cgcatcaagc gcaacgagca
      841 gagcctgcag gagatctggg actacgtgaa gcgccccaac ctgcgcctga tcggcgtgcc
      901 cgagagcgac gtggagaacg gcaccaagct ggagaacacc ctgcaggaca tcatccagga
      961 gaacttcccc aacctggccc gccaggccaa cgtgcagatc caggagatcc agcgcacccc
     1021 ccagcgctac agcagccgcc gcgccacccc ccgccacatc atcgtgcgct tcaccaaggt
     1081 ggagatgaag gagaagatgc tgcgcgccgc ccgcgagaag ggccgcgtga ccctgaaggg
     1141 caagcccatc cgtctcaccg ccgacctgag cgccgagacc ctgcaggccc gccgcgagtg
     1201 gggccccatc ttcaacatcc tgaaggagaa gaacttccag ccccgcatca gctaccccgc
     1261 caagctgagc ttcatcagcg agggcgagat caagtacttc atcgacaagc agatgctgcg
     1321 cgacttcgtg accacccgcc ccgccctgaa ggagctgctg aaggaggccc tgaacatgga
     1381 gcgcaacaac cgctaccagc ccctgcagaa ccacgccaag atgtaaagac catcgagact
     1441 aggaagaaac tgcatcaact aatgagcaaa atcaggcgcg cccatcataa tgaccggcag
     1501 caacagccac atcaccatcc tgaccctgaa catcaacggc ctgaacagcg ccatcaagcg
     1561 ccaccgcctg gccagctgga tcaagagcca ggaccccagc gtgtgctgca tccaggagac
     1621 ccacctgacc tgccgcgaca cccaccgcct gaagatcaag ggctggcgca agatctacca
     1681 ggccaacggc aagcagaaga aggccggcgt ggccatcctg gtgagcgaca agaccgactt
     1741 caagcccacc aagatcaagc gcgacaagga gggccactac atcatggtga agggcagcat
     1801 ccagcaggag gagctgacca tcctgaacat ctacgccccc aacaccggtg ccccccgctt
     1861 catcaagcag gtgctgagcg acctgcagcg cgacctggac agccacaccc tgatcatggg
     1921 cgacttcaac acccccctga gcaccctgga ccgcagcacc cgccagaagg tgaacaagga
     1981 cacccaggag ctgaacagcg ccctgcacca ggccgacctg atcgacatct accgcaccct
     2041 gcaccccaag agcaccgagt acaccttctt cagcgccccc caccacacct acagcaagat
     2101 cgaccacatc gtgggcagca aggccctgct gagcaagtgc aagcgcaccg agatcatcac
     2161 caactacctg agcgaccaca gcgccatcaa gctggagctg cgcatcaaga acctgaccca
     2221 gagccgcagc accacctgga agctgaacaa cctgctgctg aacgactact gggtgcacaa
     2281 cgagatgaag gccgagatca agatgttctt cgaaaccaac gagaacaagg acaccaccta
     2341 ccagaacctg tgggacgcct tcaaggccgt gtgccgcggc aagttcatcg ccctgaacgc
     2401 ctacaagcgc aagcaggagc gcagcaagat cgacaccctg accagccagc tgaaggagct
     2461 ggagaagcag gagcagaccc acagcaaggc cagccgccgc caggagatca ccaagatccg
     2521 cgccgagctg aaggagatcg agacccagaa gaccctgcag aagatcaacg agtcgcgaag
     2581 ctggttcttc gagcgcatca acaagatcga ccgccccctg gcccgcctga tcaagaagaa
     2641 gcgcgagaag aaccagatcg acaccatcaa gaacgacaag ggcgacatca ccaccgaccc
     2701 caccgagatc cagaccacca tccgcgagta ctacaagcac ctgtacgcca acaagctgga
     2761 gaacctggag gagatggaca ccttcctgga cacctacacc ctgccccgcc tgaaccagga
     2821 ggaggtggag agcctgaacc gccccatcac cggcagcgag atcgtggcca tcatcaacag
     2881 cctgcccacc aagaagagcc ccggccccga cggcttcacc gccgagttct accagcgcta
     2941 caaggaggag ctggtgccct tcctgctgaa gctgttccaa agcatcgaga aggagggcat
     3001 cctgcccaac agcttctacg aggccagcat catcctgatc cccaagcccg gccgcgacac
     3061 caccaagaag gagaacttcc gccccatcag cctgatgaac atcgacgcca agatcctgaa
     3121 caagatcctg gccaaccgca tccagcagca catcaagaag ctgatccacc acgaccaggt
     3181 gggcttcatc cccgggatgc agggctggtt caacatccgc aagagcatca acgtgatcca
     3241 gcacatcaac cgcgccaagg acaagaacca catgatcatc agcatcgacg ccgagaaggc
     3301 cttcgacaag atccagcagc ccttcatgct gaagaccctg aacaagctgg gcatcgacgg
     3361 cacctacttc aagatcatcc gcgccatcta cgacaagccc accgccaaca tcatcctgaa
     3421 cggccagaag ctggaggcct tccccctgaa gaccggcacg cgtcagggct gccccctgag
     3481 ccccctgctg ttcaacatcg tgctggaggt gctggcccgc gccatccgcc aggagaagga
     3541 gatcaagggc atccagctgg gcaaggagga ggtgaagctg agcctgttcg ccgacgacat
     3601 gatcgtgtac ctggagaacc ccatcgtgag cgcccagaac ctgctgaagc tgatcagcaa
     3661 cttcagcaag gtgagcggct acaagatcaa cgtgcagaag agccaggcct tcctgtacac
     3721 caacaaccgc cagaccgaga gccagatcat gggcgagctg cccttcacca tcgctagcaa
     3781 gcgcatcaag tacctgggca tccagctgac ccgcgacgtg aaggacctgt tcaaggagaa
     3841 ctacaagccc ctgctgaagg agatcaagga ggagaccaac aagtggaaga acatcccctg
     3901 cagctgggtg ggccgcatca acatcgtgaa gatggccatc ctgcccaagg tgatctaccg
     3961 cttcaacgcc atccccatca agctgcccat gaccttcttc accgagctgg agaagaccac
     4021 cctgaagttc atctggaacc agaagcgcgc ccgcatcgcc aagagcatcc tgagccagaa
     4081 gaacaaggcc ggcggcatca ccctgcccga cttcaagctg tactacaagg ccaccgtgac
     4141 caagaccgcc tggtactggt accagaaccg cgatatcgac cagtggaacc gcaccgagcc
     4201 cagcgagatc atgccccaca tctacaacta cctgatcttc gacaagcccg agaagaacaa
     4261 gcagtggggc aaggacagcc tgttcaacaa gtggtgctgg gagaactggc tggccatctg
     4321 ccgcaagctg aagctggacc ccttcctgac cccctacacc aagatcaaca gccgctggat
     4381 caaggacctg aacgtgaagc ccaagaccat caagaccctg gaggagaacc tgggcatcac
     4441 catccaggac atcggcgtgg gcaaggactt catgagcaag acccccaagg ccatggccac
     4501 caaggacaag atcgacaagt gggacctgat caagctgaag agcttctgca ccgccaagga
     4561 gaccaccatc cgcgtgaacc gccagcccac cacctgggag aagatcttcg ccacctacag
     4621 cagcgacaag ggcctgatca gccgcatcta caacgagctg aagcagatct acaagaagaa
     4681 gaccaacaac cccatcaaga agtgggccaa ggacatgaac cgccacttca gcaaggagga
     4741 catctacgcc gccaagaagc atatgaagaa gtgcagcagc agcctggcca tccgcgagat
     4801 gcagatcaag accaccatgc gctaccacct gacccccgtg cgcatggcca tcatcaagaa
     4861 gagcggcaac aaccgctgct ggcgcggctg cggcgagatc ggcaccctgc tgcactgctg
     4921 gtgggactgc aagctggtgc agcccctgtg gaagagcgtg tggcgcttcc tgcgcgacct
     4981 ggagctggag atccccttcg accccgccat ccccctgctg ggcatctacc ccaacgagta
     5041 caagagctgc tgctacaagg acacctgcac ccgcatgttc atcgccgccc tgttcaccat
     5101 cgccaagacc tggaaccagc ccaagtgccc caccatgatc gattggatca agaagatgtg
     5161 gcacatctac accatggagt actacgccgc catcaagaac gacgagttca tcagcttcgt
     5221 gggcacctgg atgaagctgg agaccatcat cctgagcaag ctgagccagg agcagaagac
     5281 caagcaccgc atcttcagcc tgatcggcgg caactgagta aaacaatgag atcacatgga
     5341 cacaggaagg ggaatatcac actctgggga ctgtggtggg gtcgggggag gggggaggga
     5401 tagcattggg agatatacct aatgctagat gacacattag tgggtgcagc gcaccagcat
     5461 ggcacatgta tacgccggca aaataccgac cagaatcatg caagtgcgta agatagtcgc
     5521 gggccgggga aaagccggca tatgtaacta acctgcacaa tgtgcacatg taccctaaaa
     5581 cttagagtat aataaaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     5641 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5701 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5761 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     5821 gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     5881 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5941 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac
     6001 tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt
     6061 cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     6121 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     6181 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     6241 tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     6301 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     6361 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     6421 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     6481 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     6541 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     6601 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     6661 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     6721 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     6781 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     6841 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     6901 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     6961 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     7021 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     7081 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     7141 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     7201 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     7261 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     7321 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     7381 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     7441 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     7501 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     7561 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     7621 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     7681 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     7741 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     7801 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     7861 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     7921 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     7981 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     8041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     8101 tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata
     8161 cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg
     8221 aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga atttaaggag
     8281 gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc
     8341 aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg
     8401 ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca
     8461 cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg
     8521 attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc
     8581 acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg
     8641 tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt
     8701 gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc
     8761 aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc
     8821 aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg
     8881 acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc
     8941 tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact
     9001 cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc
     9061 ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc
     9121 ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc
     9181 cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc
     9241 ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc
     9301 ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct
     9361 ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc
     9421 tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc
     9481 tcgaccccgg cctccacctc ctgctcctgc cnnncctcct gctcctgccc ctcctcctgc
     9541 tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt
     9601 ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct
     9661 tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct
     9721 tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc
     9781 ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc
     9841 ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca
     9901 gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt
     9961 catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt
    10021 cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt
    10081 cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg
    10141 gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg
    10201 caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa
    10261 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    10321 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
    10381 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10441 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10501 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10561 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10621 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10681 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
    10741 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10801 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10861 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10921 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10981 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
    11041 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11101 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11161 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11221 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11281 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11641 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    12001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    12061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12121 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
    12181 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
    12241 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
    12301 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
    12361 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
    12421 gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc
    12481 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
    12541 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
    12601 cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
    12661 gcgacccgct taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatggc
    12721 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
    12781 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
    12841 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
    12901 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
    12961 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
    13021 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
    13081 attgctgccc tctggttatg tgtgggaggg ctaaaacacg gaaggagaca ataccggaag
    13141 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
    13201 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
    13261 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
    13321 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
    13381 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
    13441 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
    13501 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
    13561 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
//
