LOCUS       sequence-256665-       11459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Tet-On RPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 11459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA095
FEATURES             Location/Qualifiers
     source          1..11459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        338..650
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    346..364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    382..400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    417..435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    453..471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    489..507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    524..542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    560..578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     623..647
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5660..5781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5671..5690)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5725..5744
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      6498..6507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6504..6506,6507..6509,6510..7220)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6504..6506,6507..6509,6510..7220)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6549..6570)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6810..6829)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7157..7178
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7230..7286
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7287..7904,7905..7910,7911..7946,7947..7949,7950..7985,
                     7986..7988,7989..8024,8025..8030)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             8037..8093
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8088..8888
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8148..8167)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8758..8777
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8943..8960)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8949..9173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9499..9516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9670..10258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9750..9769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10429..11220,11221..11289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11052..11071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11290..11394)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taccgcatca ggaaattgta agcgttaata ttttgttaaa attggatccc tatacagttg
       61 aagtcggaag tttacataca cttaagttgg agtcattaaa actcgttttt caactactcc
      121 acaaatttct tgttaacaaa caatagtttt ggcaagtcag ttaggacatc tactttgtgc
      181 atgacacaag tcatttttcc aacaattgtt tacagacaga ttatttcact tataattcac
      241 tgtatcacaa ttccagtggg tcagaagttt acatacacta agttcgactc ctctgcagaa
      301 tgcggcgatg tttcggtaag gggtccgcta tctagacgag tttactccct atcagtgata
      361 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
      421 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      481 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
      541 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgat taggcgtgta
      601 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagccaat
      661 tccaactctt ttgccttata cctactttcc gtaccacttc ctaccctcga aaggcctctg
      721 atgggcaaga agcagaaccg caagaccggc aacagcaaga cccagagcgc cagccccccc
      781 cccaaggagc gcagcagcag ccccgccacc gagcagagct ggatggagaa cgacttcgac
      841 gagctgcgcg aggagggctt ccgccgcagc aactacagcg agctgcgcga ggacatccag
      901 accaagggca aggaggtgga gaacttcgag aagaacctgg aggagtgcat cacccgcatc
      961 accaacaccg agaagtgcct gaaggagctg atggagctga agaccaaggc ccgcgagctg
     1021 cgcgaggagt gccgcagcct gcgcagccgc tgcgaccaat tggaggagcg cgtgagcgcc
     1081 atggaggacg agatgaacga gatgaagcgc gagggcaagt tccgcgagaa gcgcatcaag
     1141 cgcaacgagc agagcctgca ggagatctgg gactacgtga agcgccccaa cctgcgcctg
     1201 atcggcgtgc ccgagagcga cgtggagaac ggcaccaagc tggagaacac cctgcaggac
     1261 atcatccagg agaacttccc caacctggcc cgccaggcca acgtgcagat ccaggagatc
     1321 cagcgcaccc cccagcgcta cagcagccgc cgcgccaccc cccgccacat catcgtgcgc
     1381 ttcaccaagg tggagatgaa ggagaagatg ctgcgcgccg cccgcgagaa gggccgcgtg
     1441 accctgaagg gcaagcccat ccgtctcacc gccgacctga gcgccgagac cctgcaggcc
     1501 cgccgcgagt ggggccccat cttcaacatc ctgaaggaga agaacttcca gccccgcatc
     1561 agctaccccg ccaagctgag cttcatcagc gagggcgaga tcaagtactt catcgacaag
     1621 cagatgctgc gcgacttcgt gaccacccgc cccgccctga aggagctgct gaaggaggcc
     1681 ctgaacatgg agcgcaacaa ccgctaccag cccctgcaga accacgccaa gatgtaaaga
     1741 ccatcgagac taggaagaaa ctgcatcaac taatgagcaa aatcaggcgc gcccatcata
     1801 atgaccggca gcaacagcca catcaccatc ctgaccctga acatcaacgg cctgaacagc
     1861 gccatcaagc gccaccgcct ggccagctgg atcaagagcc aggaccccag cgtgtgctgc
     1921 atccaggaga cccacctgac ctgccgcgac acccaccgcc tgaagatcaa gggctggcgc
     1981 aagatctacc aggccaacgg caagcagaag aaggccggcg tggccatcct ggtgagcgac
     2041 aagaccgact tcaagcccac caagatcaag cgcgacaagg agggccacta catcatggtg
     2101 aagggcagca tccagcagga ggagctgacc atcctgaaca tctacgcccc caacaccggt
     2161 gccccccgct tcatcaagca ggtgctgagc gacctgcagc gcgacctgga cagccacacc
     2221 ctgatcatgg gcgacttcaa cacccccctg agcaccctgg accgcagcac ccgccagaag
     2281 gtgaacaagg acacccagga gctgaacagc gccctgcacc aggccgacct gatcgacatc
     2341 taccgcaccc tgcaccccaa gagcaccgag tacaccttct tcagcgcccc ccaccacacc
     2401 tacagcaaga tcgaccacat cgtgggcagc aaggccctgc tgagcaagtg caagcgcacc
     2461 gagatcatca ccaactacct gagcgaccac agcgccatca agctggagct gcgcatcaag
     2521 aacctgaccc agagccgcag caccacctgg aagctgaaca acctgctgct gaacgactac
     2581 tgggtgcaca acgagatgaa ggccgagatc aagatgttct tcgaaaccaa cgagaacaag
     2641 gacaccacct accagaacct gtgggacgcc ttcaaggccg tgtgccgcgg caagttcatc
     2701 gccctgaacg cctacaagcg caagcaggag cgcagcaaga tcgacaccct gaccagccag
     2761 ctgaaggagc tggagaagca ggagcagacc cacagcaagg ccagccgccg ccaggagatc
     2821 accaagatcc gcgccgagct gaaggagatc gagacccaga agaccctgca gaagatcaac
     2881 gagtcgcgaa gctggttctt cgagcgcatc aacaagatcg accgccccct ggcccgcctg
     2941 atcaagaaga agcgcgagaa gaaccagatc gacaccatca agaacgacaa gggcgacatc
     3001 accaccgacc ccaccgagat ccagaccacc atccgcgagt actacaagca cctgtacgcc
     3061 aacaagctgg agaacctgga ggagatggac accttcctgg acacctacac cctgccccgc
     3121 ctgaaccagg aggaggtgga gagcctgaac cgccccatca ccggcagcga gatcgtggcc
     3181 atcatcaaca gcctgcccac caagaagagc cccggccccg acggcttcac cgccgagttc
     3241 taccagcgct acaaggagga gctggtgccc ttcctgctga agctgttcca aagcatcgag
     3301 aaggagggca tcctgcccaa cagcttctac gaggccagca tcatcctgat ccccaagccc
     3361 ggccgcgaca ccaccaagaa ggagaacttc cgccccatca gcctgatgaa catcgacgcc
     3421 aagatcctga acaagatcct ggccaaccgc atccagcagc acatcaagaa gctgatccac
     3481 cacgaccagg tgggcttcat ccccgggatg cagggctggt tcaacatccg caagagcatc
     3541 aacgtgatcc agcacatcaa ccgcgccaag gacaagaacc acatgatcat cagcatcgac
     3601 gccgagaagg ccttcgacaa gatccagcag cccttcatgc tgaagaccct gaacaagctg
     3661 ggcatcgacg gcacctactt caagatcatc cgcgccatct acgacaagcc caccgccaac
     3721 atcatcctga acggccagaa gctggaggcc ttccccctga agaccggcac gcgtcagggc
     3781 tgccccctga gccccctgct gttcaacatc gtgctggagg tgctggcccg cgccatccgc
     3841 caggagaagg agatcaaggg catccagctg ggcaaggagg aggtgaagct gagcctgttc
     3901 gccgacgaca tgatcgtgta cctggagaac cccatcgtga gcgcccagaa cctgctgaag
     3961 ctgatcagca acttcagcaa ggtgagcggc tacaagatca acgtgcagaa gagccaggcc
     4021 ttcctgtaca ccaacaaccg ccagaccgag agccagatca tgggcgagct gcccttcacc
     4081 atcgctagca agcgcatcaa gtacctgggc atccagctga cccgcgacgt gaaggacctg
     4141 ttcaaggaga actacaagcc cctgctgaag gagatcaagg aggagaccaa caagtggaag
     4201 aacatcccct gcagctgggt gggccgcatc aacatcgtga agatggccat cctgcccaag
     4261 gtgatctacc gcttcaacgc catccccatc aagctgccca tgaccttctt caccgagctg
     4321 gagaagacca ccctgaagtt catctggaac cagaagcgcg cccgcatcgc caagagcatc
     4381 ctgagccaga agaacaaggc cggcggcatc accctgcccg acttcaagct gtactacaag
     4441 gccaccgtga ccaagaccgc ctggtactgg taccagaacc gcgatatcga ccagtggaac
     4501 cgcaccgagc ccagcgagat catgccccac atctacaact acctgatctt cgacaagccc
     4561 gagaagaaca agcagtgggg caaggacagc ctgttcaaca agtggtgctg ggagaactgg
     4621 ctggccatct gccgcaagct gaagctggac cccttcctga ccccctacac caagatcaac
     4681 agccgctgga tcaaggacct gaacgtgaag cccaagacca tcaagaccct ggaggagaac
     4741 ctgggcatca ccatccagga catcggcgtg ggcaaggact tcatgagcaa gacccccaag
     4801 gccatggcca ccaaggacaa gatcgacaag tgggacctga tcaagctgaa gagcttctgc
     4861 accgccaagg agaccaccat ccgcgtgaac cgccagccca ccacctggga gaagatcttc
     4921 gccacctaca gcagcgacaa gggcctgatc agccgcatct acaacgagct gaagcagatc
     4981 tacaagaaga agaccaacaa ccccatcaag aagtgggcca aggacatgaa ccgccacttc
     5041 agcaaggagg acatctacgc cgccaagaag catatgaaga agtgcagcag cagcctggcc
     5101 atccgcgaga tgcagatcaa gaccaccatg cgctaccacc tgacccccgt gcgcatggcc
     5161 atcatcaaga agagcggcaa caaccgctgc tggcgcggct gcggcgagat cggcaccctg
     5221 ctgcactgct ggtgggactg caagctggtg cagcccctgt ggaagagcgt gtggcgcttc
     5281 ctgcgcgacc tggagctgga gatccccttc gaccccgcca tccccctgct gggcatctac
     5341 cccaacgagt acaagagctg ctgctacaag gacacctgca cccgcatgtt catcgccgcc
     5401 ctgttcacca tcgccaagac ctggaaccag cccaagtgcc ccaccatgat cgattggatc
     5461 aagaagatgt ggcacatcta caccatggag tactacgccg ccatcaagaa cgacgagttc
     5521 atcagcttcg tgggcacctg gatgaagctg gagaccatca tcctgagcaa gctgagccag
     5581 gagcagaaga ccaagcaccg catcttcagc ctgatcggcg gcaactgatg tcaggccaag
     5641 cttccatcga tagacatgat aagatacatt gatgagtttg gacaaaccac aacaagaatg
     5701 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5761 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     5821 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tacctaggcg
     5881 agaccctgtc tcacaaaata aagtaagccc ggactgagtg cggaaaggcg ggcctggcgg
     5941 gtctggtctc cccatgcggg ccaccagagg ccctgcagcc ttcagtcgct tgaaggggta
     6001 atggcgcttc cactcacaaa catggcggac agagcgtgtg aacgagatga acagcccctc
     6061 aaaaatatgg ccgccgaggc tggacggccg tgccccagca gcaccgcctc cgcgccccac
     6121 gtgatctctc gccgggcaca gcgctgaccg cggaggtcca accggaagaa tgtccggatt
     6181 ggacattcgg aagagggccc gccttccctg gggaatctct gcgcacgcgc agaacgcttc
     6241 gaccaatgaa aacacaggaa gccgtccgcg caaccgcgtt gcgtcacttc tgccgcccct
     6301 gtttcaaggt atatagccgt agacggaact tcgcctttct ctcggcctta gcgccatttt
     6361 tttgggtgag tgttttttgg ttcctgcgtt gggattccgt gtacaatcca tagacatctg
     6421 acctcggcac ttagcatcat cacagcaaac taactgtagc ctttctctct ttccctgtag
     6481 aaacctctgc acctgaggcc accatggtga gcaagggcga ggagctgttc accggggtgg
     6541 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     6601 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     6661 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     6721 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     6781 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     6841 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     6901 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     6961 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     7021 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     7081 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     7141 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     7201 gcatggacga gctgtacaag ggcagtggag ctactaactt cagcctgctg aagcaggctg
     7261 gtgacgtcga ggagaatcct ggccccatgt ctagactgga caagagcaaa gtcataaacg
     7321 gcgctctgga attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc
     7381 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     7441 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     7501 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct
     7561 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     7621 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     7681 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     7741 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     7801 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     7861 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg gccgacgccc
     7921 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     7981 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg tccggcgcta
     8041 ctaacttcag cctgctgaag caggctggtg acgtcgagga gaatcctggt cccatgattg
     8101 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     8161 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     8221 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     8281 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     8341 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     8401 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     8461 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     8521 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     8581 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     8641 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     8701 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     8761 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     8821 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     8881 tcttctgatt cgaaggcctg tcgtgaagct tggggatcaa ttctctagag ctcgctgatc
     8941 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9001 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9061 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9121 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9181 ggcggaaaga accagctggg cctactagct actcgggacc ccttaccgaa acatcgccgc
     9241 attctgcaga ggagtcgagt gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa
     9301 ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat
     9361 aaagtggtga tcctaactga cctaagacag ggaattttta ctaggattaa atgtcaggaa
     9421 ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac
     9481 tgtataggga tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10081 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10441 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10501 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10561 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10621 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10681 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10741 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10801 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10861 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10921 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10981 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11041 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11101 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11161 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11221 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11281 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11341 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11401 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaa
//
