LOCUS       sequence-256667-       13406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13406)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST51-LRRK2-G2019S
FEATURES             Location/Qualifiers
     source          1..13406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8882..8906)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8932..8973
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8983..9000
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9023..9040)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9029..9253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9299..9727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9386..9405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9596..9617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9736..9756)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9741..10070
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9921..10056
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9983..10002
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10118..10165
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10184..10582
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10740..10861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10777..10796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10831..10850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10910..10926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10910..10926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10923..10945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10934..10950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10958..10964,10965..10982,10983..10988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11003..11024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11141..11158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11301..11889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11381..11400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12060..12851,12852..12920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12683..12702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12921..13025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13100..13119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt gatcaacaag tttgtacaaa
     1261 aaagcaggct ccgaattcgc ccttatggct agtggcagct gtcaggggtg cgaagaggac
     1321 gaggaaactc tgaagaagtt gatagtcagg ctgaacaatg tccaggaagg aaaacagata
     1381 gaaacgctgg tccaaatcct ggaggatctg ctggtgttca cgtactccga gcacgcctcc
     1441 aagttatttc aaggcaaaaa tatccatgtg cctctgttga tcgtcttgga ctcctatatg
     1501 agagtcgcga gtgtgcagca ggtgggttgg tcacttctgt gcaaattaat agaagtctgt
     1561 ccaggtacaa tgcaaagctt aatgggaccc caggatgttg gaaatgattg ggaagtcctt
     1621 ggtgttcacc aattgattct taaaatgcta acagttcata atgccagtgt aaacttgtca
     1681 gtgattggac tgaagacctt agatctcctc ctaacttcag gtaaaatcac cttgctgata
     1741 ttggatgaag aaagtgatat tttcatgtta atttttgatg ccatgcactc atttccagcc
     1801 aatgatgaag tccagaaact tggatgcaaa gctttacatg tgctgtttga gagagtctca
     1861 gaggagcaac tgactgaatt tgttgagaac aaagattata tgatattgtt aagtgcgtta
     1921 acaaatttta aagatgaaga ggaaattgtg cttcatgtgc tgcattgttt acattcccta
     1981 gcgattcctt gcaataatgt ggaagtcctc atgagtggca atgtcaggtg ttataatatt
     2041 gtggtggaag ctatgaaagc attccctatg agtgaaagaa ttcaagaagt gagttgctgt
     2101 ttgctccata ggcttacatt aggtaatttt ttcaatatcc tggtattaaa cgaagtccat
     2161 gagtttgtgg tgaaagctgt gcagcagtac ccagagaatg cagcattgca gatctcagcg
     2221 ctcagctgtt tggccctcct cactgagact attttcttaa atcaagattt agaggaaaag
     2281 aatgagaatc aagagaatga tgatgagggg gaagaagata aattgttttg gctggaagcc
     2341 tgttacaaag cattaacgtg gcatagaaag aacaagcacg tgcaggaggc cgcatgctgg
     2401 gcactaaata atctccttat gtaccaaaac agtttacatg agaagattgg agatgaagat
     2461 ggccatttcc cagctcatag ggaagtgatg ctctccatgc tgatgcattc ttcatcaaag
     2521 gaagttttcc aggcatctgc gaatgcattg tcaactctct tagaacaaaa tgttaatttc
     2581 agaaaaatac tgttatcaaa aggaatacac ctgaatgttt tggagttaat gcagaagcat
     2641 atacattctc ccgaagtggc tgaaagtggc tgtaaaatgc taaatcatct ttttgaagga
     2701 agcaacactt ccctggatat aatggcagca gtggtcccca aaatactaac agttatgaaa
     2761 cgtcatgaga catcattacc agtgcagctg gaggcgcttc gagctatttt acattttata
     2821 gtgcctggca tgccagaaga atccagggag gatacagaat ttcatcataa gctaaatatg
     2881 gttaaaaaac agtgtttcaa gaatgatatt cacaaactgg tcctagcagc tttgaacagg
     2941 ttcattggaa atcctgggat tcagaaatgt ggattaaaag taatttcttc tattgtacat
     3001 tttcctgatg cattagagat gttatccctg gaaggtgcta tggattcagt gcttcacaca
     3061 ctgcagatgt atccagatga ccaagaaatt cagtgtctgg gtttaagtct tataggatac
     3121 ttgattacaa agaagaatgt gttcatagga actggacatc tgctggcaaa aattctggtt
     3181 tccagcttat accgatttaa ggatgttgct gaaatacaga ctaaaggatt tcagacaatc
     3241 ttagcaatcc tcaaattgtc agcatctttt tctaagctgc tggtgcatca ttcatttgac
     3301 ttagtaatat tccatcaaat gtcttccaat atcatggaac aaaaggatca acagtttcta
     3361 aacctctgtt gcaagtgttt tgcaaaagta gctatggatg attacttaaa aaatgtgatg
     3421 ctagagagag cgtgtgatca gaataacagc atcatggttg aatgcttgct tctattggga
     3481 gcagatgcca atcaagcaaa ggagggatct tctttaattt gtcaggtatg tgagaaagag
     3541 agcagtccca aattggtgga actcttactg aatagtggat ctcgtgaaca agatgtacga
     3601 aaagcgttga cgataagcat tgggaaaggt gacagccaga tcatcagctt gctcttaagg
     3661 aggctggccc tggatgttgc caacaatagc atttgccttg gaggattttg tataggaaaa
     3721 gttgaacctt cttggcttgg tcctttattt ccagataaga cttctaattt aaggaaacaa
     3781 acaaatatag catctacact agcaagaatg gtgatcagat atcagatgaa aagtgctgtg
     3841 gaagaaggaa cagcctcagg cagcgatgga aatttttctg aagatgtgct gtctaaattt
     3901 gatgaatgga cctttattcc tgactcttct atggacagtg tgtttgctca aagtgatgac
     3961 ctggatagtg aaggaagtga aggctcattt cttgtgaaaa agaaatctaa ttcaattagt
     4021 gtaggagaat tttaccgaga tgccgtatta cagcgttgct caccaaattt gcaaagacat
     4081 tccaattcct tggggcccat ttttgatcat gaagatttac tgaagcgaaa aagaaaaata
     4141 ttatcttcag atgattcact caggtcatca aaacttcaat cccatatgag gcattcagac
     4201 agcatttctt ctctggcttc tgagagagaa tatattacat cactagacct ttcagcaaat
     4261 gaactaagag atattgatgc cctaagccag aaatgctgta taagtgttca tttggagcat
     4321 cttgaaaagc tggagcttca ccagaatgca ctcacgagct ttccacaaca gctatgtgaa
     4381 actctgaaga gtttgacaca tttggacttg cacagtaata aatttacatc atttccttct
     4441 tatttgttga aaatgagttg tattgctaat cttgatgtct ctcgaaatga cattggaccc
     4501 tcagtggttt tagatcctac agtgaaatgt ccaactctga aacagtttaa cctgtcatat
     4561 aaccagctgt cttttgtacc tgagaacctc actgatgtgg tagagaaact ggagcagctc
     4621 attttagaag gaaataaaat atcagggata tgctccccct tgagactgaa ggaactgaag
     4681 attttaaacc ttagtaagaa ccacatttca tccctatcag agaactttct tgaggcttgt
     4741 cctaaagtgg agagtttcag tgccagaatg aattttcttg ctgctatgcc tttcttgcct
     4801 ccttctatga caatcctaaa attatctcag aacaaatttt cctgtattcc agaagcaatt
     4861 ttaaatcttc cacacttgcg gtctttagat atgagcagca atgatattca gtacctacca
     4921 ggtcccgcac actggaaatc tttgaactta agggaactct tatttagcca taatcagatc
     4981 agcatcttgg acttgagtga aaaagcatat ttatggtcta gagtagagaa actgcatctt
     5041 tctcacaata aactgaaaga gattcctcct gagattggct gtcttgaaaa tctgacatct
     5101 ctggatgtca gttacaactt ggaactaaga tcctttccca atgaaatggg gaaattaagc
     5161 aaaatatggg atcttccttt ggatgaactg catcttaact ttgattttaa acatatagga
     5221 tgtaaagcca aagacatcat aaggtttctt caacagcgat taaaaaaggc tgtgccttat
     5281 aaccgaatga aacttatgat tgtgggaaat actgggagtg gtaaaaccac cttattgcag
     5341 caattaatga aaaccaagaa atcagatctt ggaatgcaaa gtgccacagt tggcatagat
     5401 gtgaaagact ggcctatcca aataagagac aaaagaaaga gagatctcgt cctaaatgtg
     5461 tgggattttg caggtcgtga ggaattctat agtactcatc cccattttat gacgcagcga
     5521 gcattgtacc ttgctgtcta tgacctcagc aagggacagg ctgaagttga tgccatgaag
     5581 ccttggctct tcaatataaa ggctcgcgct tcttcttccc ctgtgattct cgttggcaca
     5641 catttggatg tttctgatga gaagcaacgc aaagcctgca tgagtaaaat caccaaggaa
     5701 ctcctgaata agcgagggtt ccctgccata cgagattacc actttgtgaa tgccaccgag
     5761 gaatctgatg ctttggcaaa acttcggaaa accatcataa acgagagcct taatttcaag
     5821 atccgagatc agcttgttgt tggacagctg attccagact gctatgtaga acttgaaaaa
     5881 atcattttat cggagcgtaa aaatgtgcca attgaatttc ccgtaattga ccggaaacga
     5941 ttattacaac tagtgagaga aaatcagctg cagttagatg aaaatgagct tcctcacgca
     6001 gttcactttc taaatgaatc aggagtcctt cttcattttc aagacccagc actgcagtta
     6061 agtgacttgt actttgtgga acccaagtgg ctttgtaaaa tcatggcaca gattttgaca
     6121 gtgaaagtgg aaggttgtcc aaaacaccct aagggaatta tttcgcgtag agatgtggaa
     6181 aaatttcttt caaaaaaaag gaaatttcca aagaactaca tgtcacagta ttttaagctc
     6241 ctagaaaaat tccagattgc tttgccaata ggagaagaat atttgctggt tccaagcagt
     6301 ttgtctgacc acaggcctgt gatagagctt ccccattgtg agaactctga aattatcatc
     6361 cgactatatg aaatgcctta ttttccaatg ggattttggt caagattaat caatcgatta
     6421 cttgagattt caccttacat gctttcaggg agagaacgag cacttcgccc aaacagaatg
     6481 tattggcgac aaggcattta cttaaattgg tctcctgaag cttattgtct ggtaggatct
     6541 gaagtcttag acaatcatcc agagagtttc ttaaaaatta cagttccttc ttgtagaaaa
     6601 ggctgtattc ttttgggcca agttgtggac cacattgatt ctctcatgga agaatggttt
     6661 cctgggttgc tggagattga tatttgtggt gaaggagaaa ctctgttgaa gaaatgggca
     6721 ttatatagtt ttaatgatgg cgaagaacat caaaaaatct tacttgatga cttgatgaag
     6781 aaagcagagg aaggagatct cttagtaaat ccagatcaac caaggctcac cattccaata
     6841 tctcagattg cccctgactt gattttggct gacctgccta gaaatattat gttgaataat
     6901 gatgagttgg aatttgaaca agctccagag tttctcctag gtgatggcag ttttggatca
     6961 gtttaccgag cagcctatga aggagaagaa gtggctgtga agatttttaa taaacataca
     7021 tcactcaggc tgttaagaca agagcttgtg gtgctttgcc acctccacca ccccagtttg
     7081 atatctttgc tggcagctgg gattcgtccc cggatgttgg tgatggagtt agcctccaag
     7141 ggttccttgg atcgcctgct tcagcaggac aaagccagcc tcactagaac cctacagcac
     7201 aggatcgcac tccacgtagc tgatggtttg agatacctcc actcagccat gattatatac
     7261 cgagacctga aaccccacaa tgtgctgctt ttcacactgt atcccaatgc tgccatcatc
     7321 gcaaagattg ctgactacag cattgctcag tactgctgta gaatggggat aaaaacatca
     7381 gagggcacac cagggtttcg tgcacctgaa gttgccagag gaaatgtcat ttataaccaa
     7441 caggctgatg tttattcatt tggtttacta ctctatgaca ttttgacaac tggaggtaga
     7501 atagtagagg gtttgaagtt tccaaatgag tttgatgaat tagaaataca aggaaaatta
     7561 cctgatccag ttaaagaata tggttgtgcc ccatggccta tggttgagaa attaattaaa
     7621 cagtgtttga aagaaaatcc tcaagaaagg cctacttctg cccaggtctt tgacattttg
     7681 aattcagctg aattagtctg tctgacgaga cgcattttat tacctaaaaa cgtaattgtt
     7741 gaatgcatgg ttgctacaca tcacaacagc aggaatgcaa gcatttggct gggctgtggg
     7801 cacaccgaca gaggacagct ctcatttctt gacttaaata ctgaaggata cacttctgag
     7861 gaagttgctg atagtagaat attgtgctta gccttggtgc atcttcctgt tgaaaaggaa
     7921 agctggattg tgtctgggac acagtctggt actctcctgg tcatcaatac cgaagatggg
     7981 aaaaagagac ataccctaga aaagatgact gattctgtca cttgtttgta ttgcaattcc
     8041 ttttccaagc aaagcaaaca aaaaaatttt cttttggttg gaaccgctga tggcaagtta
     8101 gcaatttttg aagataagac tgttaagctt aaaggagctg ctcctttgaa gatactaaat
     8161 ataggaaatg tcagtactcc attgatgtgt ttgagtgaat ccacaaattc aacggaaaga
     8221 aatgtaatgt ggggaggatg tggcacaaag attttctcct tttctaatga tttcaccatt
     8281 cagaaactca ttgagacaag aacaagccaa ctgttttctt atgcagcttt cagtgattcc
     8341 aacatcataa cagtggtggt agacactgct ctctatattg ctaagcaaaa tagccctgtt
     8401 gtggaagtgt gggataagaa aactgaaaaa ctctgtggac taatagactg cgtgcacttt
     8461 ttaagggagg taatggtaaa agaaaacaag gaatcaaaac acaaaatgtc ttattctggg
     8521 agagtgaaaa ccctctgcct tcagaagaac actgctcttt ggataggaac tggaggaggc
     8581 catattttac tcctggatct ttcaactcgt cgacttatac gtgtaattta caacttttgt
     8641 aattcggtca gagtcatgat gacagcacag ctaggaagcc ttaaaaatgt catgctggta
     8701 ttgggctaca accggaaaaa tactgaaggt acacaaaagc agaaagagat acaatcttgc
     8761 ttgaccgttt gggacatcaa tcttccacat gaagtgcaaa atttagaaaa acacattgaa
     8821 gtgagaaaag aattagctga aaaaatgaga cgaacatctg ttgagaaaaa gggcgaattc
     8881 gacccagctt tcttgtacaa agtggttgat ctagagggcc cgcggttcga aggtaagcct
     8941 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     9001 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     9061 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     9121 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     9181 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     9241 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     9301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9361 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9421 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9481 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9601 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9721 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9781 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9901 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9961 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    10021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    10081 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    10141 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
    10201 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
    10261 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
    10321 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
    10381 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
    10441 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
    10501 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
    10561 tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga cacgtgctac
    10621 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    10681 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    10741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10861 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    10921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10981 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    11041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    11101 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    11161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    11221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag gaaccgtaaa
    11281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    11761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13021 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    13081 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    13141 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    13201 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    13261 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa
    13321 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
    13381 tctaggtctt gaaaggagtg ggaatt
//
