LOCUS       sequence-256671-       10374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual yTRAP, Gateway destination vector of aTF2 fusion reporting on 
            mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pSK234
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    419..440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    495..511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     500..522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     519..535
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     519..535
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        830..1237
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     protein_bind    1790..1914
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1939..1944,1945..1962,1963..1969)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2023..2682
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2089..2108)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3024..3329
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3208..3227
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3370..3494)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3532..3552
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4156..4245
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4252..4341
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5900..6087
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5955..5979)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             6511..7320
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6578..6597)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7837..7853
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7837..7853
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7867..7886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7868..7886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7893..7910)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7893..7909)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7902..7924)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8209..8228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8341..8362
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8516..8535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8635..8657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8695..8713)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8781..8885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8886..8954,8955..9746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9104..9123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9917..10374,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      421 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccgcggctcg
      481 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      541 ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc cgaggagaac
      601 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacgatta
      661 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt
      721 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt
      781 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa
      841 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc
      901 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
      961 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
     1021 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
     1081 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
     1141 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
     1201 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agatggcgtc
     1261 gtacgctgca ccgagtgcaa ccacacaaaa atcatacaac gaatggtata tgcttcattt
     1321 ctttgtttcg cattagctgc gctatttgac tcaaattatt attttttact aagacgacgc
     1381 gtcacagtgt tcgagtctgt gtcatttctt ttgtaattct cttaaaccac ttcataaagt
     1441 tgtgaagttc atagcaaaat tcttccgcaa aaagatgaat cttagttctc agcccaccaa
     1501 aagaggtaca tgctaagatc atacagaagt tattgtcact tcttaccttg ctcttaaatg
     1561 tacattacaa ccgggtatta tatcttacat catcgtataa tatgatcttt ctttatggag
     1621 aaaatttttt tttcactcga ccaaagctcc cattgcttct gaagagtgta gtgtatattg
     1681 gtacatcttc tcttgaaaga ctccattgta ctgtaacaaa aagcggtttc ttcatcgact
     1741 tgctcggaat aacatctata tctgcccact agcaacagct agccccatca caagtttgta
     1801 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1861 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     1921 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1981 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2041 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2101 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2161 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2221 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2281 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2341 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2401 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2461 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2521 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2581 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2641 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2701 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2761 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2821 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2881 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2941 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     3001 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     3061 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     3121 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3181 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     3241 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3301 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3361 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3421 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3481 ttgtacaaag tggtgatggg ctgcaggaat tcgatatcaa gcttaatggc tccaaagaag
     3541 aagagaaagg ttggtattca tggtgttcca gttgatggtg gtttacaagc tccaccaact
     3601 gatgtttctt tgggtgatga attgcatttg gatggtgaag atgttgctat ggctcatgct
     3661 gatgctttgg atgattttga tttggatatg ttgggtgacg gtgattctcc aggtccaggt
     3721 tttactccac atgattctgc tccatatggt gctttagata tggctgactt tgaattcgaa
     3781 caaatgttca ccgatgcctt gggtattgat gaatatggtg gtggtgctag acccggggag
     3841 cgccccttcc agtgtcgcat ttgcatgcgg aacttttcga gaaatttcat tttgcaaaga
     3901 catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc
     3961 tccgatagag ctaatttgag aagacatcta cgtacgcaca ccggcgagaa gccattccaa
     4021 tgccgaatat gcatgcgcaa cttcagtaga catgatcaat tgactagaca cctaaaaacc
     4081 cacctgagag gttctgccgg cgaattggct ttgaaattgg ctggtttgga tatcaattct
     4141 ggttctgctg ctagataccc atatgatgtt ccagattatg ctggttaccc ttacgatgtt
     4201 cctgactacg caggttctta tccatacgat gtacctgatt atgcctcaag atatccttat
     4261 gacgtccctg attacgccgg ttatccatat gacgtaccag actacgctgg tagttaccca
     4321 tacgacgtcc cagattacgc ttcttcataa tgagggccct catgtaattt cttgcaaaca
     4381 taagtaaatg caaacacaat aataccgatc ataaagcatt ttcttctata ttaaaaaaca
     4441 aggtttaata aagctgttat atatatatat atatatatag acgtataatt agtttagttc
     4501 tttttgtacc atataccata aacaaggtaa acttcacctc tcaatatatc tagaatttca
     4561 taaaaatatc tagcaaggtt tcaactcctt caatcacgtt ttcatcactc aaccctatct
     4621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct
     4741 gatgcggtat tttctttaat taatgaggac gtgtagttta attgaggacg tgtggttcta
     4801 aagtgaggac gtgtattaaa tatgaggacg tgtttaaagc attgaggacg tgtcctaaat
     4861 ctgaggacgt gtaattatct gaggacgtgt tttagaagtg aggacgtgtg gccggcccag
     4921 atccgccagg cgtgtatata tagcgtggat ggccaggcaa ctttagtgct gacacataca
     4981 ggcatatata tatgtgtgcg acgacacatg atcatatggc atgcatgtgc tctgtatgta
     5041 tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt
     5101 gcagcataaa ttactatact tctatagaca cacaaacaca aatacacaca ctgtttaaac
     5161 aaaaaatggg ttctggtggt tctgaattga tcaaagaaaa catgcacatg aagttgtaca
     5221 tggaaggtac tgttaacaac catcatttca agtgtacctc tgaaggtgaa ggtaaaccat
     5281 atgaaggtac tcaaaccatg agaatcaaag ctgttgaagg tggtccattg ccatttgctt
     5341 ttgatatttt ggctacctcc ttcatgtacg gttctaagac tttcattaac cacacccaag
     5401 gtatcccaga tttcttcaaa caatctttcc cagaaggttt cacttgggaa agagttacta
     5461 cttacgaaga tggtggtgtt ttgactgcta ctcaagatac ttctttacaa gacggttgct
     5521 tgatctacaa cgttaagatt agaggtgtca acttcccatc taatggtcca gttatgcaaa
     5581 aaaagacttt gggttgggaa gcttctactg aaacattata tccagctgat ggtggtttgg
     5641 aaggtagagc tgatatggct ttgaaattgg ttggtggtgg tcatttgatc tgcaacttga
     5701 aaactaccta cagatctaag aagccagcca agaatttgaa aatgccaggt gtttactacg
     5761 tcgatagaag attggaaaga atcaaagaag ccgacaaaga aacctacgtt gaacaacacg
     5821 aagttgctgt tgctagatat tgtgatttgc catccaaatt aggtcataga ggatcttaag
     5881 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     5941 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     6001 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
     6061 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat
     6121 cgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     6181 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     6241 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     6301 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     6361 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     6421 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     6481 catcacatcc gaacatcaat tgaaacaacc atgggtaagg aaaagactca cgtttcgagg
     6541 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     6601 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     6661 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     6721 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     6781 gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa
     6841 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     6901 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     6961 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     7021 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     7081 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     7141 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     7201 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     7261 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     7321 agatcttcag tactgacaat aaaaagattg cttaaaaaga ttctcttttt ttatgatatt
     7381 tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa
     7441 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     7501 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     7561 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     7621 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     7681 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     7741 taagtcaata cctccttgaa ccatttccca taatggtgaa agttccctca agaattttac
     7801 tctgtcagaa acggccttaa cgacgtaggc ggccgctcta gaactagtgg atcgatcccc
     7861 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     7921 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7981 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8041 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     8101 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8161 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     8221 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggtg
     8281 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8341 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     8401 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     8461 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     8521 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8701 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcg
//
