LOCUS       sequence-256672-        6098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 161_pAAV-ProD5-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        327..358
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      412..421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             418..1344
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1348..1350,1351..1353,1354..2067)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1348..1350,1351..1353,1354..2064)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1393..1414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1654..1673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2095..2683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2148..2168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2566..2577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2715..3191
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2914..2933)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3231..3371
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3231..3360
                     /label=AAV2 ITR
     rep_origin      3446..3901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3533..3552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3743..3764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3918..3937)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4037..4059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4097..4115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4183..4287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4288..4356,4357..5148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4506..4525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5319..5907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5808..5827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5969..6098
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag aagaggaagt
       61 caaacaggac tagtcatcga taggctagcc agcttaatta atcagatttc caggaagagg
      121 caccagagcc ctctccctat tgctcaaagc gcaggtggaa taatcttggc cgtgtgaccg
      181 gaattcaaag cagttttggg aactgagccc caacctgtaa gccctgatgt tatttcaaga
      241 aagatggtgt cagaattaaa ttggattaga gaggaggata tggattagag cagagcagca
      301 tcctcacatg gtcctgctgg agttagtaga gggtatataa tggaagctcg acttccagct
      361 atcacatcca ctgtgttgtt gtgaactgga atccactata ggccaggatc cgccaccatg
      421 gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta
      481 gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag
      541 tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc
      601 ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg
      661 gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt cttcttcgag
      721 tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac
      781 gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct gacgggcttg
      841 tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg cacaattgtg
      901 tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt
      961 ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac
     1021 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
     1081 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
     1141 gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt
     1201 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
     1261 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
     1321 gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg
     1381 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1441 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1501 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1561 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1621 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1681 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1741 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1801 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1861 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1921 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1981 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2041 ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat cgataatcaa
     2101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
     2521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg
     2701 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     2761 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     2821 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     2881 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     2941 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     3001 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     3061 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     3121 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     3181 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     3241 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     3301 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     3361 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3421 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     3541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3661 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     3781 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     3841 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3901 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3961 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4021 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4081 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4141 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4201 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4261 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4321 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4381 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4441 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4501 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4561 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4621 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4681 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4741 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4801 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4861 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4921 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4981 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5041 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5101 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5161 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5221 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5281 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5341 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5401 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5461 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5521 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5581 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5641 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5701 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5761 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5821 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5881 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5941 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
     6001 gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     6061 cagagaggga gtggccaact ccatcactag gggttcct
//
