LOCUS       sequence-256673-        6398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 159_pAAV-ProD3-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        411..442
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      496..505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             502..1428
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1432..1434,1435..1437,1438..2151)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1432..1434,1435..1437,1438..2148)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1477..1498)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1738..1757)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2179..2767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2232..2252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2650..2661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2799..3275
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2998..3017)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3315..3455
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3315..3444
                     /label=AAV2 ITR
     rep_origin      3530..3985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3617..3636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3827..3848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4002..4021)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4121..4143
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4181..4199)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4267..4371
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4372..4440,4441..5232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4590..4609)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5403..5991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5892..5911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6053..6182
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 tgtgtccctg gtgaaaagtc taccccctcg ggtttgttat ggggcccaga acttgtacaa
       61 tgccccgtaa gcttcttaga ggaggcgggt attaggcagg attggatctg cgtcccctga
      121 catgcccttc atacccttga attaggattc ctaagggagc cggcaggagg agaatggggt
      181 gttccttgtg gttccacttc acacctactc ttcgagaccc cggattctga caagtttccc
      241 gaacttggaa gacccagatg tcaaggtctg aaggtaaatg atgtgtttag gcaggagggt
      301 tcagccaggc tcagctctcc tcccctctcg caggggaagg agagagaccc tctagaattc
      361 ctgtaggagg tgcacgtggg ctgatcctca catggtcctg ctggagttag tagagggtat
      421 ataatggaag ctcgacttcc agctatcaca tccactgtgt tgttgtgaac tggaatccac
      481 tataggccag gatccgccac catggattat ggaggcgccc tgagtgccgt tgggcgcgag
      541 ctgctatttg taacgaaccc agtagtcgtc aatggctctg tacttgtgcc tgaggaccag
      601 tgttactgcg cgggctggat tgagtcgcgt ggcacaaacg gtgcccaaac ggcgtcgaac
      661 gtgctgcaat ggcttgctgc tggcttctcc atcctactgc ttatgtttta cgcctaccaa
      721 acatggaagt caacctgcgg ctgggaggag atctatgtgt gcgctatcga gatggtcaag
      781 gtgattctcg agttcttctt cgagtttaag aacccgtcca tgctgtatct agccacaggc
      841 caccgcgtcc agtggttgcg ttacgccgag tggcttctca cctgcccggt catttgcatt
      901 cacctgtcaa acctgacggg cttgtccaac gactacagca ggcgcaccat gggtctgctt
      961 gtgtctgata ttggcacaat tgtgtggggc gccacttccg ccatggccac cggatacgtc
     1021 aaggtcatct tcttctgcct gggtctgtgt tatggtgcta acacgttctt tcacgctgcc
     1081 aaggcctaca tcgagggtta ccacaccgtg ccgaagggcc ggtgtcgcca ggtggtgact
     1141 ggcatggctt ggctcttctt cgtatcatgg ggtatgttcc ccatcctgtt catcctcggc
     1201 cccgagggct tcggcgtcct gagcgtgtac ggctccaccg tcggccacac catcattgac
     1261 ctgatgtcga agaactgctg gggtctgctc ggccactacc tgcgcgtgct gatccacgag
     1321 catatcctca tccacggcga cattcgcaag accaccaaat tgaacattgg tggcactgag
     1381 attgaggtcg agacgctggt ggaggacgag gccgaggctg gcgcggtacc catggtgagc
     1441 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1501 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1561 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1621 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1681 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1741 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1801 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1861 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1921 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1981 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2041 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2101 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg ataagaattc
     2161 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2581 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     2641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2701 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2761 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     2821 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     2881 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     2941 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3001 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3061 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3121 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3181 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3241 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3301 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3361 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3421 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3481 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3541 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3601 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3661 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3721 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     3781 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3841 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     3901 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3961 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4021 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4081 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4141 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4201 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4261 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4321 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4381 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4441 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4501 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4561 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4621 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4681 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4741 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4801 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4861 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4921 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4981 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5041 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5101 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5161 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5221 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5281 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5341 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5521 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5761 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5881 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5941 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6001 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg
     6061 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc
     6121 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
     6181 ctgcggccgc acgcgtatcc tggggtttaa acaggactag gtccactggg agaagaggaa
     6241 gtcaaacagg actagtcatc gataggctag ccagcttaat taatcagatt gtctttcctc
     6301 ccgtctaagc atggaagccc taaatggcct acaattccta tagcttagtt gcaaatttga
     6361 gaagttcccc cactgactct ggtggaggtg gtctattc
//
