LOCUS       sequence-256676-        6332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 198_pAAV-ProD1-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        366..397
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      451..460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             457..1383
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1387..1389,1390..1392,1393..2106)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1387..1389,1390..1392,1393..2103)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1432..1453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1693..1712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2134..2722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2187..2207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2605..2616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2754..3230
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2953..2972)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3270..3410
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3270..3399
                     /label=AAV2 ITR
     rep_origin      3485..3940
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3572..3591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3782..3803
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3957..3976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4076..4098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4136..4154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4222..4326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4327..4395,4396..5187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4545..4564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5358..5946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5847..5866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6008..6137
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 agagcaaata aaactgtctt ctctagtttg gggaaattgg agcctgcctt agggaaacag
       61 tgactaattt acagctagtg tttggaatga gatcatctgt caaaataaat gtatctttac
      121 ttccttcttg agaggaagca agctgtttta tgatgttcct tggaatcctt aaaaatacag
      181 agcaacattt acattattaa tgatacgctt tattgctgca ggctaactag gtccaaaatt
      241 gtccttccat agatggagct ggagagttac acagaagttt gcatatcgag ctcttaggtc
      301 tgcatgtaca gctaatgtac ttgtggaccc tgtcacatat cctcacatgg tcctgctgga
      361 gttagtagag ggtatataat ggaagctcga cttccagcta tcacatccac tgtgttgttg
      421 tgaactggaa tccactatag gccaggatcc gccaccatgg attatggagg cgccctgagt
      481 gccgttgggc gcgagctgct atttgtaacg aacccagtag tcgtcaatgg ctctgtactt
      541 gtgcctgagg accagtgtta ctgcgcgggc tggattgagt cgcgtggcac aaacggtgcc
      601 caaacggcgt cgaacgtgct gcaatggctt gctgctggct tctccatcct actgcttatg
      661 ttttacgcct accaaacatg gaagtcaacc tgcggctggg aggagatcta tgtgtgcgct
      721 atcgagatgg tcaaggtgat tctcgagttc ttcttcgagt ttaagaaccc gtccatgctg
      781 tatctagcca caggccaccg cgtccagtgg ttgcgttacg ccgagtggct tctcacctgc
      841 ccggtcattt gcattcacct gtcaaacctg acgggcttgt ccaacgacta cagcaggcgc
      901 accatgggtc tgcttgtgtc tgatattggc acaattgtgt ggggcgccac ttccgccatg
      961 gccaccggat acgtcaaggt catcttcttc tgcctgggtc tgtgttatgg tgctaacacg
     1021 ttctttcacg ctgccaaggc ctacatcgag ggttaccaca ccgtgccgaa gggccggtgt
     1081 cgccaggtgg tgactggcat ggcttggctc ttcttcgtat catggggtat gttccccatc
     1141 ctgttcatcc tcggccccga gggcttcggc gtcctgagcg tgtacggctc caccgtcggc
     1201 cacaccatca ttgacctgat gtcgaagaac tgctggggtc tgctcggcca ctacctgcgc
     1261 gtgctgatcc acgagcatat cctcatccac ggcgacattc gcaagaccac caaattgaac
     1321 attggtggca ctgagattga ggtcgagacg ctggtggagg acgaggccga ggctggcgcg
     1381 gtacccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1441 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1501 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1561 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1621 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1681 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1741 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1801 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1861 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1921 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1981 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2041 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2101 aagtgataag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2521 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     2581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2701 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     2761 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     2821 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     2881 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     2941 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3001 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3061 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3121 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3181 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     3241 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     3301 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3361 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3421 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3481 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3541 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     3601 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3661 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3721 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3781 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     3841 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     3901 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     3961 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6001 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
     6061 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     6121 catcactagg ggttcctgcg gccgcacgcg tatcctgggg tttaaacagg actaggtcca
     6181 ctgggagaag aggaagtcaa acaggactag tcatcgatag gctagccagc ttaattaatc
     6241 agatggttca ttgtaagcca ctgtagtcgt gggtgactca catcaaacca ccccttcggg
     6301 aacacgatgc cgacactgaa actacatagg gg
//
