LOCUS       sequence-256677-        6535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 13_pAAV-ProC71-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      849..858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             855..1781
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1785..1787,1788..1790,1791..2504)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1785..1787,1788..1790,1791..2501)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1830..1851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2091..2110)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2438..2459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2532..3120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2585..2605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3003..3014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3152..3628
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3351..3370)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3668..3808
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3668..3797
                     /label=AAV2 ITR
     rep_origin      3883..4338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3970..3989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4180..4201
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4355..4374)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4474..4496
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4534..4552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4620..4724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4725..4793,4794..5585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4943..4962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5756..6344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6245..6264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6406..6535
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc aaacgccctc
       61 ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc tccccgatcc
      121 ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc tcctaccctc
      181 ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg cccctgagac
      241 ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct gttgcttggc
      301 ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg ttatgtacac
      361 ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga cctgaccgtc
      421 ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata ttgaatagtc
      481 ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg ggcgtgctgc
      541 ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct cgccactcac
      601 ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt ggaatagatc
      661 ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt aacttcattc
      721 ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaatatata atggaagctt
      781 gaccggagcc gttagttgcc cgtcggacgc gatttgcgag gcgctttatg cgcgcaccgg
      841 tcggatccgc caccatggat tatggaggcg ccctgagtgc cgttgggcgc gagctgctat
      901 ttgtaacgaa cccagtagtc gtcaatggct ctgtacttgt gcctgaggac cagtgttact
      961 gcgcgggctg gattgagtcg cgtggcacaa acggtgccca aacggcgtcg aacgtgctgc
     1021 aatggcttgc tgctggcttc tccatcctac tgcttatgtt ttacgcctac caaacatgga
     1081 agtcaacctg cggctgggag gagatctatg tgtgcgctat cgagatggtc aaggtgattc
     1141 tcgagttctt cttcgagttt aagaacccgt ccatgctgta tctagccaca ggccaccgcg
     1201 tccagtggtt gcgttacgcc gagtggcttc tcacctgccc ggtcatttgc attcacctgt
     1261 caaacctgac gggcttgtcc aacgactaca gcaggcgcac catgggtctg cttgtgtctg
     1321 atattggcac aattgtgtgg ggcgccactt ccgccatggc caccggatac gtcaaggtca
     1381 tcttcttctg cctgggtctg tgttatggtg ctaacacgtt ctttcacgct gccaaggcct
     1441 acatcgaggg ttaccacacc gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg
     1501 cttggctctt cttcgtatca tggggtatgt tccccatcct gttcatcctc ggccccgagg
     1561 gcttcggcgt cctgagcgtg tacggctcca ccgtcggcca caccatcatt gacctgatgt
     1621 cgaagaactg ctggggtctg ctcggccact acctgcgcgt gctgatccac gagcatatcc
     1681 tcatccacgg cgacattcgc aagaccacca aattgaacat tggtggcact gagattgagg
     1741 tcgagacgct ggtggaggac gaggccgagg ctggcgcggt acccatggtg agcaagggcg
     1801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1861 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1921 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1981 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2041 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2101 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2221 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2281 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2341 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2401 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2461 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgataagaa ttcgatatca
     2521 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2941 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3061 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3121 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     3181 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     3241 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     3301 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     3361 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     3421 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     3481 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     3541 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     3601 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     3661 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3721 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3781 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     3841 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3901 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     3961 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4021 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4081 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4141 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4201 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4261 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4321 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4381 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4441 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4501 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5881 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc
     6421 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca
     6481 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcct
//
