LOCUS       sequence-256679-        2116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Linus, Part 4a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM582
FEATURES             Location/Qualifiers
     source          1..2116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(481..515)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(586..1245)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1160..1179
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1427..2015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1916..1935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcatcgg tctcaatcct tggctactac acttgaacgt attgagaaga actttgtcat
       61 tactgaccca aggttgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt
      121 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga
      181 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac
      241 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca
      301 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac
      361 tgagcatgtc cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga
      421 agagattgcc aaaaagaaga gaaaggtcta actcgagtgg ctgagacctt gaccagacca
      481 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      541 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      601 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      661 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      721 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
      781 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
      841 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
      901 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
      961 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1021 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1081 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1141 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1201 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1261 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1321 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     1381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2101 ctgtggataa ccgtag
//
