LOCUS       sequence-256683-        2263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Linus-Mxi, Part 4a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM725
FEATURES             Location/Qualifiers
     source          1..2263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             479..601
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(628..662)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(733..1392)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1307..1326
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1574..2162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2063..2082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccttggcta ctacacttga acgtattgag aagaactttg tcattactga
       61 cccaaggttg ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga
      121 atatagccgt gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga
      181 tcgcgcgaca gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca
      241 gctgattaat tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat
      301 gcgagatcag aagggagatg tccagtactt tattggggtt cagttggatg gaactgagca
      361 tgtccgagat gctgccgaga gagagggagt catgctgatt aagaaaactg cagaagagat
      421 tgccaaaaag aagagaaagg tcggcggcag cggcggcggc agcggcagcg gcggcagcat
      481 ggaacgtgtg agaatgatta atgtgcaaag gctgttagaa gccgcagagt ttttagaaag
      541 aagagaaaga gaatgcgaac acgggtatgc cagttctttc cctagcatgc cctctcccag
      601 ataactcgag tggctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
      661 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      721 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      781 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      841 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      901 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
      961 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1021 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1081 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1141 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1201 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1261 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1321 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1381 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1441 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
     1501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tag
//
