LOCUS       sequence-256696-        5778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9, Part 3a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM585
FEATURES             Location/Qualifiers
     source          1..5778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4143..4177)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4248..4907)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4822..4841
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5089..5677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5578..5597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggacaaga agtattctat cggactggcc atcgggacta atagcgtcgg
       61 gtgggccgtg atcactgacg agtacaaggt gccctctaag aagttcaagg tgctcgggaa
      121 caccgaccgg cattccatca agaaaaatct gatcggagct ctcctctttg attcagggga
      181 aaccgctgaa gcaacccgcc tcaagcggac tgctagacgg cggtacacca ggaggaagaa
      241 ccggatttgt taccttcaag agatattctc caacgaaatg gcaaaggtcg acgacagctt
      301 cttccatagg ctggaagaat cattcctcgt ggaagaggat aagaagcatg aacggcatcc
      361 catcttcggt aatatcgtcg acgaggtggc ctatcacgag aaatacccaa ccatctacca
      421 tcttcgcaaa aagctggtgg actcaaccga caaggcagac ctccggctta tctacctggc
      481 cctggcccac atgatcaagt tcagaggcca cttcctgatc gagggcgacc tcaatcctga
      541 caatagcgat gtggataaac tgttcatcca gctggtgcag acttacaacc agctctttga
      601 agagaacccc atcaatgcaa gcggagtcga tgccaaggcc attctgtcag cccggctgtc
      661 aaagagccgc agacttgaga atcttatcgc tcagctgccg ggtgaaaaga aaaatggact
      721 gttcgggaac ctgattgctc tttcacttgg gctgactccc aatttcaagt ctaatttcga
      781 cctggcagag gatgccaagc tgcaactgtc caaggacacc tatgatgacg atctcgacaa
      841 cctcctggcc cagatcggtg accaatacgc cgaccttttc cttgctgcta agaatctttc
      901 tgacgccatc ctgctgtctg acattctccg cgtgaacact gaaatcacca aggcccctct
      961 ttcagcttca atgattaagc ggtatgatga gcaccaccag gacctgaccc tgcttaaggc
     1021 actcgtccgg cagcagcttc cggagaagta caaggaaatc ttctttgacc agtcaaagaa
     1081 tggatacgcc ggctacatcg acggaggtgc ctcccaagag gaattttata agtttatcaa
     1141 acctatcctt gagaagatgg acggcaccga agagctcctc gtgaaactga atcgggagga
     1201 tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt ccccaccaga tccatcttgg
     1261 ggagcttcac gccatccttc ggcgccaaga ggacttctac ccctttctta aggacaacag
     1321 ggagaagatt gagaaaattc tcactttccg catcccctac tacgtgggac ccctcgccag
     1381 aggaaatagc cggtttgctt ggatgaccag aaagtcagaa gaaactatca ctccctggaa
     1441 cttcgaagag gtggtggaca agggagccag cgctcagtca ttcatcgaac ggatgactaa
     1501 cttcgataag aacctcccca atgagaaggt cctgccgaaa cattccctgc tctacgagta
     1561 ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc
     1621 cgcattcctg tcaggcgaac aaaagaaggc aattgtggac cttctgttca agaccaatag
     1681 aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag aaaattgaat gcttcgactc
     1741 tgtggagatt agcggggtcg aagatcggtt caacgcaagc ctgggtacct accatgatct
     1801 gcttaagatc atcaaggaca aggattttct ggacaatgag gagaaagagg acatccttga
     1861 ggacattgtc ctgactctca ctctgttcga ggaccgggaa atgatcgagg agaggcttaa
     1921 gacctacgcc catctgttcg acgataaagt gatgaagcaa cttaaacgga gaagatatac
     1981 cggatgggga cgccttagcc gcaaactcat caacggaatc cgggacaaac agagcggaaa
     2041 gaccattctt gatttcctta agagcgacgg attcgctaat cgcaacttca tgcaacttat
     2101 ccatgatgat tccctgacct ttaaggagga catccagaag gcccaagtgt ctggacaagg
     2161 tgactcactg cacgagcata tcgcaaatct ggctggttca cccgctatta agaagggtat
     2221 tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga
     2281 gaacattgtc atcgagatgg ccagggaaaa ccagactacc cagaagggac agaagaacag
     2341 cagggagcgg atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac agatccttaa
     2401 agagcacccg gtggaaaaca cccagcttca gaatgagaag ctctatctgt actaccttca
     2461 aaatggacgc gatatgtatg tggaccaaga gcttgatatc aacaggctct cagactacga
     2521 cgtggacgcc atcgtccctc agagcttcct caaagacgac tcaattgaca ataaggtgct
     2581 gactcgctca gacaagaacc ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa
     2641 aaagatgaag aactattggc gccagcttct gaacgcaaag ctgatcactc agcggaagtt
     2701 cgacaatctc actaaggctg agaggggcgg actgagcgaa ctggacaaag caggattcat
     2761 taaacggcaa cttgtggaga ctcggcagat tactaaacat gtagcccaaa tccttgactc
     2821 acgcatgaat accaagtacg acgaaaacga caaacttatc cgcgaggtga aggtgattac
     2881 cctgaagtcc aagctggtca gcgatttcag aaaggacttt caattctaca aagtgcggga
     2941 gatcaataac tatcatcatg ctcatgacgc atatctgaat gccgtggtgg gaaccgccct
     3001 gatcaagaag tacccaaagc tggaaagcga gttcgtgtac ggagactaca aggtctacga
     3061 cgtgcgcaag atgattgcca aatctgagca ggagatcgga aaggccaccg caaagtactt
     3121 cttctacagc aacatcatga atttcttcaa gaccgaaatc acccttgcaa acggtgagat
     3181 ccggaagagg ccgctcatcg agactaatgg ggagactggc gaaatcgtgt gggacaaggg
     3241 cagagatttc gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa
     3301 aaccgaggtg caaaccggag gcttttctaa ggaatcaatc ctccccaagc gcaactccga
     3361 caagctcatt gcaaggaaga aggattggga ccctaagaag tacggcggat tcgattcacc
     3421 aactgtggct tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct
     3481 caagagcgtg aaggaactgc tgggtatcac cattatggag cgcagctcct tcgagaagaa
     3541 cccaattgac tttctcgaag ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa
     3601 gctcccaaag tatagcctgt tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc
     3661 tggcgaactt cagaagggta atgagctggc tctcccctcc aagtacgtga atttcctcta
     3721 ccttgcaagc cattacgaga agctgaaggg gagccccgag gacaacgagc aaaagcaact
     3781 gtttgtggag cagcataagc attatctgga cgagatcatt gagcagattt ccgagttttc
     3841 taaacgcgtc attctcgctg atgccaacct cgataaagtc cttagcgcat acaataagca
     3901 cagagacaaa ccaattcggg agcaggctga gaatatcatc cacctgttca ccctcaccaa
     3961 tcttggtgcc cctgccgcat tcaagtactt cgacaccacc atcgaccgga aacgctatac
     4021 ctccaccaaa gaagtgctgg acgccaccct catccaccag agcatcaccg gactttacga
     4081 aactcggatt gacctctcac agctcggagg ggatgaggga gctggttctt gagaccagac
     4141 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     4201 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     4261 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     4321 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     4381 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     4441 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
     4501 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     4561 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     4621 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     4681 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     4741 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     4801 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     4861 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     4921 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     4981 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     5041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5761 ttctgtggat aaccgtag
//
