LOCUS       sequence-256709-        6501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 42_pAAV-ProC41-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        612..808
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      659..794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             821..1747
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1751..1753,1754..1756,1757..2470)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1751..1753,1754..1756,1757..2467)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1796..1817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2057..2076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2404..2425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2498..3086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2551..2571)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2969..2980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3118..3594
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3317..3336)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3634..3774
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3634..3763
                     /label=AAV2 ITR
     rep_origin      3849..4304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3936..3955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4146..4167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4321..4340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4440..4462
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4500..4518)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4586..4690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4691..4759,4760..5551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4909..4928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5722..6310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6211..6230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6372..6501
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgttagact ggagtgccaa tgcataaatt cattgctagc tattaaatat
       61 gcataaattc atctccgtgg ccaatacatg cataaattca tgcggggtcg cccttaatgc
      121 ataaattcat tgtgttaaat atagtatgca taaattcata gcgtcggaac cggtatgcat
      181 aaattcattt taccgacttt tgaatgcata aattcattta gacacacaaa atatgcataa
      241 attcattcga ccccgcagct catgcataaa ttcatccgta cggttcctgc atgcataaat
      301 tcattgtttt tatggaaata tgcataaatt catgtcaagc acgtaaccat gcataaattc
      361 atacttgatg gttgggcatg cataaattca tggtgttaaa tgttatatgc ataaattcat
      421 cctccaaatc actacatgca taaattcatg aaagacgtcg ggtaatgcat aaattcatcc
      481 atttatcgta accatgcata aattcatgag acgattgggc ctatgcataa attcataact
      541 gaagaattcc gatgcataaa ttcatcagga acacgatacg atgcataaat tcatgctcga
      601 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      661 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt
      721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      781 cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct
      841 gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt
      901 acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg
      961 tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct
     1021 tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg
     1081 cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat
     1141 gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac
     1201 ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag
     1261 gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc
     1321 catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa
     1381 cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg
     1441 gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc
     1501 catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt
     1561 cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct
     1621 gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt
     1681 gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg
     1741 cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1801 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1861 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1921 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1981 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2041 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2101 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2161 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2221 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2281 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2341 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2401 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2461 gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2521 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2581 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2641 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2701 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2761 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2821 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2881 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     2941 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3001 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3061 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     3121 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3181 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3241 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3301 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3361 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     3421 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3481 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3541 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     3601 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3661 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3721 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     3781 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     3841 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3901 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     3961 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4021 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4081 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4141 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     4201 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     4261 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4321 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4381 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4441 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4501 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4561 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4621 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4681 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4741 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4801 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4861 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4921 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4981 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5041 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5101 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5161 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5221 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5281 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5341 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5401 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5461 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5521 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5581 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5641 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5701 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5761 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5821 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5881 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5941 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6001 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6061 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6361 tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc
     6421 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
     6481 actccatcac taggggttcc t
//
