LOCUS       sequence-256720-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human RAB10 with N-terminal GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, 
            Jimenez-Morales D, Stevenson E, Krogan NJ, Swaney DL, Snyder MP, 
            Mukherjee S, Bassik MC
  TITLE     Systematic Identification of Host Cell Regulators of Legionella 
            pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
  JOURNAL   Cell Host Microbe. 2019 Oct 9;26(4):551-563.e6. doi: 
            10.1016/j.chom.2019.08.017. Epub 2019 Sep 17.
  PUBMED    31540829
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.08.017"; journalName: "Cell Host Microbe"; 
            date: "2019-10-9- 9"; volume: "26"; issue: "4"; pages: "551-563.e6"
COMMENT     Sequence Label: GFP-RAB10
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..254
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          255..1193
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1150..1170
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1214..1810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1214..1233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1710..1730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1820..1873
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1880..1889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1886..1888,1889..1891,1892..2602)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1931..1952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2192..2211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2539..2560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3237..3270)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3326..3914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3379..3399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3797..3808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3917..3933)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3918..3934)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4443..4623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4685..5273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4765..4784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5444..6235,6236..6304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6067..6086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6305..6409)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6484..6503)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6675..7054
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7056..7254
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7209..7229
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7272..7452
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7499..7624
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8121..8354
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8539..8583
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8881..8998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgtggata accgtattac cgccatgcat gtgcccgtca gtgggcagag cgcacatcgc
       61 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      121 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      181 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      241 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
      301 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
      361 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
      421 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
      481 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
      541 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
      601 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
      661 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
      721 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
      781 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
      841 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
      901 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
      961 gggcgccgtc caggcacctc gattagttct cgcgcttttg gagtacgtcg tctttaggtt
     1021 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     1081 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     1141 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     1201 gagctagccc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1261 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1321 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     1561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1801 gcccggtgcc ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     1861 gaatcctggc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1921 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg
     1981 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2041 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2101 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2161 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2221 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2281 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2341 tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat
     2401 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2461 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2521 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2581 cggcatggac gagctgtaca agggtggaag tggtatggcg aagaagacgt acgacctgct
     2641 tttcaagctg ctcctgatcg gggattccgg agtggggaag acctgcgtcc tttttcgttt
     2701 ttcggatgat gccttcaata ctacctttat ttccaccata ggaatagact tcaagatcaa
     2761 aacagttgaa ttacaaggaa agaagatcaa gctacagata tgggatacag caggccagga
     2821 gcgatttcac accatcacaa cctcctacta cagaggcgca atgggtatca tgctagtata
     2881 tgacatcacc aatggtaaaa gttttgaaaa catcagcaaa tggcttagaa acatagatga
     2941 gcatgccaat gaagatgtgg aaagaatgtt actaggaaac aagtgtgata tggacgacaa
     3001 aagagttgta cctaaaggaa aaggagaaca gattgcaagg gagcatggta ttaggttttt
     3061 tgagactagt gcaaaagcaa atataaacat cgaaaaggcg ttcctcacgt tagctgaaga
     3121 tatccttcga aagacccctg taaaagagcc caacagtgaa aatgtagata tcagcagtgg
     3181 aggaggcgtg acaggctgga agagcaaatg ctgctgagaa ttcgtcgagg gacctaataa
     3241 cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt tccactaggt
     3301 acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3721 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3841 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3901 ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa
     3961 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4021 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4081 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4141 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4201 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     4261 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     4321 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     4381 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     4441 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4501 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4561 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4621 gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6421 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     6481 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     6541 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     6601 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     6661 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     6721 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     6781 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     6841 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     6901 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     6961 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     7021 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     7081 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     7141 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     7201 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     7261 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7441 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7501 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7561 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7621 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7681 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     7741 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     7801 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     7861 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     7921 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     7981 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8041 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8101 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8161 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8221 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8281 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8341 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8401 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8461 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8521 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8581 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8641 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8701 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     8761 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     8821 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     8881 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8941 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9001 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc ttcgagtact
     9061 aggatccatt aggcggc
//
