LOCUS       sequence-256721-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human RAB10 T23N mutant with N-terminal GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, 
            Jimenez-Morales D, Stevenson E, Krogan NJ, Swaney DL, Snyder MP, 
            Mukherjee S, Bassik MC
  TITLE     Systematic Identification of Host Cell Regulators of Legionella 
            pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
  JOURNAL   Cell Host Microbe. 2019 Oct 9;26(4):551-563.e6. doi: 
            10.1016/j.chom.2019.08.017. Epub 2019 Sep 17.
  PUBMED    31540829
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.08.017"; journalName: "Cell Host Microbe"; 
            date: "2019-10-9- 9"; volume: "26"; issue: "4"; pages: "551-563.e6"
COMMENT     Sequence Label: GFP-RAB10 T23N
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    192..309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        431..642
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          643..1581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1538..1558
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1602..2198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1602..1621)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2098..2118
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2208..2261
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2268..2277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2274..2276,2277..2279,2280..2990)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2319..2340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2580..2599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2927..2948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3625..3658)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3714..4302
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3767..3787)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4185..4196)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4305..4321)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4306..4322)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4831..5011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5073..5661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5153..5172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5832..6623,6624..6692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6455..6474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6693..6797)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6872..6891)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7063..7442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7444..7642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7597..7617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7660..7840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7887..8012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8509..8742
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8927..8971
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
       61 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      121 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
      181 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      241 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      301 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
      361 cttcgagtac taggatccat taggcggccg cgtggataac cgtattaccg ccatgcatgt
      421 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      481 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      541 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      601 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      661 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      721 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      781 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      841 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      901 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      961 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     1021 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     1081 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1141 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1201 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1261 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1321 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1381 cgcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1441 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1501 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1561 agtttttttc ttccatttca ggtgtcgtga gctagcccac catgaccgag tacaagccca
     1621 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     1681 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     1741 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     1801 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     1861 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     1921 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     1981 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     2041 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     2101 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     2161 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atcgggagag ggcagaggaa
     2221 gtctgctaac atgcggtgac gtcgaggaga atcctggccc accggtcgcc accatggtga
     2281 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2341 taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc
     2401 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2461 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2521 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2581 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2641 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2701 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2761 aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2821 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2881 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2941 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggaagtg
     3001 gtatggcgaa gaagacgtac gacctgcttt tcaagctgct cctgatcggg gattccggag
     3061 tggggaagaa ctgcgtcctt tttcgttttt cggatgatgc cttcaatact acctttattt
     3121 ccaccatagg aatagacttc aagatcaaaa cagttgaatt acaaggaaag aagatcaagc
     3181 tacagatatg ggatacagca ggccaggagc gatttcacac catcacaacc tcctactaca
     3241 gaggcgcaat gggtatcatg ctagtatatg acatcaccaa tggtaaaagt tttgaaaaca
     3301 tcagcaaatg gcttagaaac atagatgagc atgccaatga agatgtggaa agaatgttac
     3361 taggaaacaa gtgtgatatg gacgacaaaa gagttgtacc taaaggaaaa ggagaacaga
     3421 ttgcaaggga gcatggtatt aggttttttg agactagtgc aaaagcaaat ataaacatcg
     3481 aaaaggcgtt cctcacgtta gctgaagata tccttcgaaa gacccctgta aaagagccca
     3541 acagtgaaaa tgtagatatc agcagtggag gaggcgtgac aggctggaag agcaaatgct
     3601 gctgagaatt cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac
     3661 atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac
     3721 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3781 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3841 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3901 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3961 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4021 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4081 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4141 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4201 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4261 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4321 gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4381 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4441 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4501 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     4561 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     4621 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     4681 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     4741 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     4801 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     4861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4921 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4981 cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc
     5041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     6841 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6901 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     6961 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     7021 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     7081 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     7141 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7201 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7261 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     7321 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     7381 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     7441 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     7501 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     7561 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     7621 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     7681 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7741 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7801 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     7861 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     7921 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     7981 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8041 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8101 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8161 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8221 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8281 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8341 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8401 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8461 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8521 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8581 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8641 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8701 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8761 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8821 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8881 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8941 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9001 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9061 tgctgtactt tctatag
//
