LOCUS       sequence-256722-        6334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 174_pAAV-ProC39-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        445..641
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      492..627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             654..1580
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1584..1586,1587..1589,1590..2303)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1584..1586,1587..1589,1590..2300)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1629..1650)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1890..1909)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2237..2258
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2331..2919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2384..2404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2802..2813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2951..3427
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3150..3169)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3467..3607
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3467..3596
                     /label=AAV2 ITR
     rep_origin      3682..4137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3769..3788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3979..4000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4154..4173)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4273..4295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4333..4351)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4419..4523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4524..4592,4593..5384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4742..4761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5555..6143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6044..6063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6205..6334
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgttccaag gcacccttta taaatcaata acgatctaac acctgacgaa
       61 ttaattaata acgatccgcg tcgtaggcat aaatcaataa ctgtattact gaatgcgaat
      121 taattaataa cgcgattgta gcgcactata aatcaataaa ctggggtttc gtgccgaatt
      181 aattaataac gctttaggct aagtggataa atcaataaat atgctctgtt atccgaatta
      241 attaataacg gaccatgatg gattcataaa tcaataaggg tcgaaccaga agcgaattaa
      301 ttaataacgc cattagctag caagataaat caataatgaa ctactagttg acgaattaat
      361 taataacgtg gtcgatgcga tctataaatc aataagtccg ctcgaacaac cgaattaatt
      421 aataacggct cgagatctgc gatctgcatc tcaattagtc agcaaccata gtcccgcccc
      481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatcgct
      541 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aggatccgcc accatggatt
      661 atggaggcgc cctgagtgcc gttgggcgcg agctgctatt tgtaacgaac ccagtagtcg
      721 tcaatggctc tgtacttgtg cctgaggacc agtgttactg cgcgggctgg attgagtcgc
      781 gtggcacaaa cggtgcccaa acggcgtcga acgtgctgca atggcttgct gctggcttct
      841 ccatcctact gcttatgttt tacgcctacc aaacatggaa gtcaacctgc ggctgggagg
      901 agatctatgt gtgcgctatc gagatggtca aggtgattct cgagttcttc ttcgagttta
      961 agaacccgtc catgctgtat ctagccacag gccaccgcgt ccagtggttg cgttacgccg
     1021 agtggcttct cacctgcccg gtcatttgca ttcacctgtc aaacctgacg ggcttgtcca
     1081 acgactacag caggcgcacc atgggtctgc ttgtgtctga tattggcaca attgtgtggg
     1141 gcgccacttc cgccatggcc accggatacg tcaaggtcat cttcttctgc ctgggtctgt
     1201 gttatggtgc taacacgttc tttcacgctg ccaaggccta catcgagggt taccacaccg
     1261 tgccgaaggg ccggtgtcgc caggtggtga ctggcatggc ttggctcttc ttcgtatcat
     1321 ggggtatgtt ccccatcctg ttcatcctcg gccccgaggg cttcggcgtc ctgagcgtgt
     1381 acggctccac cgtcggccac accatcattg acctgatgtc gaagaactgc tggggtctgc
     1441 tcggccacta cctgcgcgtg ctgatccacg agcatatcct catccacggc gacattcgca
     1501 agaccaccaa attgaacatt ggtggcactg agattgaggt cgagacgctg gtggaggacg
     1561 aggccgaggc tggcgcggta cccatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1801 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2041 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2101 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2281 gcatggacga gctgtacaag tgataagaat tcgatatcaa gcttatcgat aatcaacctc
     2341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     2761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2881 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     2941 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     3001 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     3061 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     3121 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     3181 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     3241 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     3301 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     3361 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     3421 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
     3481 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3541 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     3601 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3661 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3721 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     3781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     3901 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3961 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
     4021 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
     4081 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4141 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4261 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4321 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4381 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4741 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4801 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4861 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4921 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4981 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5041 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5101 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5161 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5221 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5281 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5341 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5401 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5461 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5521 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5701 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5941 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6181 tttgctggcc ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg
     6241 ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
     6301 gagggagtgg ccaactccat cactaggggt tcct
//
