LOCUS       sequence-256738-        9028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pcDNA5 Flag-NFRKB
FEATURES             Location/Qualifiers
     source          1..9028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..930
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             937..963
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4980..4997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4986..5210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5343..5362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5494..5541
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5549..6571
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5549..6571
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6701..6822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6738..6757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6792..6811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6871..6887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6871..6887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6884..6906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6895..6911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6919..6925,6926..6943,6944..6949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6964..6985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7102..7119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7273..7861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7353..7372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8032..8823,8824..8892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8655..8674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8893..8997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgccagact acaaggacga tgatgacaag cttaagtacc catacgatgt tccagattac
      961 gctctcgaca tggattcctt agaccatatg ctgacagatc ctctggaact tggtccgtgt
     1021 ggagatggcc atggcacgcg catcatggag gattgcctcc tgggaggcac cagagttagt
     1081 ctgcccgagg accttctgga ggatcctgag atcttctttg atgttgtcag cctctcaaca
     1141 tggcaggaag tgttaagtga ttctcaacgt gaacacctcc agcagtttct gccccagttt
     1201 cctgaagaca gtgctgagca gcagaatgaa ctcatcttag ccttgttcag tggggagaac
     1261 ttccgctttg gaaaccctct gcacattgcc cagaagcttt tccgagacgg acactttaac
     1321 cccgaggtgg tcaagtaccg gcagttatgc ttcaagtcac agtacaagcg ctacctcaac
     1381 tcccagcagc agtatttcca tcggctgctg aagcaaattc ttgcttcccg gagtgatctg
     1441 ctggagatgg cccggcggag tggccccgcc cttcccttcc ggcagaaacg cccttcacca
     1501 tcccgcacac ctgaggagcg ggagtggcgg acccagcagc gctacttgaa ggtcttaagg
     1561 gaagtgaaag aggagtgtgg tgacacagcc ctgtcatctg atgaagagga tctcagctca
     1621 tggcttccga gctctccagc acgttctcct agtcctgcgg tgcccctgcg ggtggtgccc
     1681 acactttcaa ccacggatat gaaaactgca gataaagtag aactggggga cagtgacctg
     1741 aagataatgt taaagaagca ccacgagaag cggaaacatc agccagatca cccggacctt
     1801 ttgacagggg acctgactct caatgacatc atgactcgag taaatgctgg caggaagggc
     1861 tctctggcag ccttatatga cttggctgtc cttaaaaaaa aggttaagga aaaagaggaa
     1921 aagaagaaga agaaaataaa aacgatcaaa tcagaggcag aggacctggc cgagccgcta
     1981 agcagtactg aaggggtcgc acctctctca caggccccct ctccgctggc aattcctgct
     2041 atcaaggaag agccccttga agacctcaag ccttgccttg gaatcaatga aatatcttcc
     2101 agcttcttct ctcttctatt agagatcttg ctgctggaga gtcaggctag ccttcctatg
     2161 ctagaggagc gagttttgga ttggcagtca tcgccagcca gctccctcaa cagctggttc
     2221 tctgcggccc ccaactgggc tgagttggta ctaccagccc tgcagtatct tgctggagaa
     2281 agtcgagctg ttccttccag tttctctcca tttgttgaat tcaaagagaa aacccagcag
     2341 tggaagttgc ttggccaatc ccaagataat gaaaaggaat tagctgccct cttccagcta
     2401 tggctagaga ccaaagatca ggccttctgt aagcaagaaa atgaagacag ctcagatgcc
     2461 acaacacctg tccctcgggt aagaactgac tatgtggtgc gtcccagcac gggggaggag
     2521 aaacgggttt ttcaggagca ggagcgttac aggtatagcc aaccccataa ggcgttcacc
     2581 ttccgcatgc acggctttga gtctgtggtg gggccagtga agggcgtgtt tgacaaggag
     2641 acctcgctca acaaggctcg ggagcactcc ctgctgcgct ccgaccggcc tgcctacgtc
     2701 accattctgt ctcttgttcg ggacgctgcg gctcgactgc ctaatggaga aggcacacgg
     2761 gcagagatct gtgaactgct taaggactcc cagtttcttg caccagatgt caccagcact
     2821 caggtaaata cagtagtgag tggtgcactg gatcggctac attacgaaaa agatccctgt
     2881 gtgaaatacg acattggacg aaagctgtgg atctacctgc atcgtgaccg gagtgaagaa
     2941 gagtttgagc ggattcacca agcacaagca gctgcagcta aagccagaaa agctcttcag
     3001 caaaaaccca agcccccatc caaggtgaag tccagtagca aggagagctc cataaaggtc
     3061 cttagcagtg gcccttctga gcagagccag atgagcctca gtgactccag tatgccaccc
     3121 accccagtca cacctgtaac ccccaccaca ccagcattgc ccgccattcc catctcccct
     3181 ccacctgtat cggcagtgaa caaaagcggc ccttccacag tctcagaacc agctaagtct
     3241 agctcgggtg ttcttctggt gtcttcacca acaatgccac atctgggaac aatgctttcc
     3301 ccagcttcca gccagactgc acccagttct caggctgccg cccgggtcgt gagccactct
     3361 ggctctgctg gactgtctca ggtgcgagtg gtggcccagc ctagccttcc cgctgttccc
     3421 cagcagtcgg gagggccggc acagacattg ccacagatgc cagcaggacc gcagatccgg
     3481 gttccagcca ctgccacaca gaccaaagta gtgccccaga cagtaatggc cactgtgccc
     3541 gtcaaagcgc agactacggc agccactgtg cagcggcctg gacccgggca gacagggctc
     3601 acggtgacaa gtctccctgc cacagccagc cctgtgagta agccagccac gagttctcct
     3661 gggacctctg ctcccagtgc ctccacggct gccgtcattc aaaatgtcac aggacagaac
     3721 atcatcaagc aggtggcaat cactgggcag cttggtgtga agccccaaac aggcaacagc
     3781 attccactca cagccactaa cttccgcatc cagggtaagg atgtattgcg tctgccgccc
     3841 tcttccatca ccacagatgc caagggccag acggttctgc gaatcactcc ggacatgatg
     3901 gccacattgg ccaagtccca ggttaccaca gtcaaattga cccaggacct cttcgggaca
     3961 ggaggcaaca ctacaggcaa aggcatctct gccaccttac acgtcacttc caatccagta
     4021 catgcagctg atagccctgc caaggccagt tcagccagtg ccccttcatc cactccaaca
     4081 ggtaccactg tggtcaaagt gactcctgac ctcaagccaa cagaagcctc aagttcggct
     4141 tttcgcttga tgccagctct tggcgtgagt gtggctgacc agaagggaaa aagcacagtg
     4201 gcctcttcag aagcaaaacc agctgccacg atccgcatcg tgcagggact gggagtgatg
     4261 cctcccaaag caggccagac catcaccgtt gcaacccacg ccaagcaagg ggcctcggtg
     4321 gccagtgggt ctggaactgt ccatacttca gcggtgtcct tacccagtat gaatgctgct
     4381 gtgtccaaga ctgtagctgt ggcttctggg gctgcaagca cccccatcag catcagcaca
     4441 ggagccccca ccgtgcggca ggtccctgtc agcaccacgg ttgtgtccac gtcccaggct
     4501 gagaagttgc ctacacggat cacagttccc ctctctgtga tcagccagcc aatgaagggc
     4561 aagagcgcgg tcacagcccc catcatcaaa ggcaaccttg gagccgacct cagtgggttg
     4621 ggccgcaaca tcatcctcac aactatgcca gcaggcacta agctcattgc tggcaataag
     4681 cctgttagtt tcctcactgc tcagcagttg cagcagcttc agcagcaagg tcaggccaca
     4741 caggtgcgca tccagactgt ccctgcatcc catctccaac agggaacagc ttctggctcc
     4801 tccaaagcag tctccactgt tgttgtgact acagctccgt ctcctaaaca ggcacctgag
     4861 caacaatgag ctctagagct gcaggcggat ccactagtcc agtgtggtgg aattctgcag
     4921 atatccagca cagtggcggc cgcttaagtc tagagggccc gtttaaaccc gctgatcagc
     4981 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5041 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5101 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5161 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5221 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5401 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5461 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     5521 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     5581 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     5641 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     5701 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     5761 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     5821 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     5881 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     5941 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     6001 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     6061 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     6121 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     6181 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     6241 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     6301 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     6361 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     6421 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     6481 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     6541 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     6601 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6661 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6841 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6901 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6961 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7021 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7081 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7141 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7201 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7261 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7441 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7681 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7861 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7921 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7981 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8041 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8101 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8161 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8221 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8281 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8341 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8401 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8461 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8521 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8581 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8641 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8701 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8761 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8821 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8881 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8941 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9001 atttccccga aaagtgccac ctgacgtc
//
