LOCUS       sequence-256739-        6541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 120_pAAV-ProC26-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        440..636
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      487..622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      643..652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             649..1575
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1579..1581,1582..1584,1585..2298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1579..1581,1582..1584,1585..2295)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1624..1645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1885..1904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2232..2253
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2326..2914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2379..2399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2797..2808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2946..3422
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3145..3164)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3462..3602
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3462..3591
                     /label=AAV2 ITR
     rep_origin      3677..4132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3764..3783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3974..3995
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4149..4168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4268..4290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4328..4346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4414..4518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4519..4587,4588..5379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4737..4756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5550..6138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6039..6058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6200..6329
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 tggataaata aagatccatt aatacggctg tcgtataaat aaagatccat cgccattctg
       61 ctcaaataaa taaagatcca tctgactgcg agtgtaataa ataaagatcc atgcaccggc
      121 tcgtgctata aataaagatc cattagctcg cggatgtaat aaataaagat ccatctgcgt
      181 tgatattaca taaataaaga tccatttctg gtcttgccct ataaataaag atccatcgtc
      241 aactgcttgc gataaataaa gatccatgta gccatgaagt cgataaataa agatccattt
      301 tactctcaat ttcataaata aagatccatt cagattggtg tgccataaat aaagatccat
      361 ttcaaagctg agtgcataaa taaagatcca tcaggagcct ggctgaataa ataaagatcc
      421 atgctcgaga tctgcgatct gcatctcaat tagtcagcaa ccatagtccc gcccctaact
      481 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tcgctgacta
      541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
      601 tgaggaggct tttttggagg cctaggcttt tgcaaaggat ccgccaccat ggattatgga
      661 ggcgccctga gtgccgttgg gcgcgagctg ctatttgtaa cgaacccagt agtcgtcaat
      721 ggctctgtac ttgtgcctga ggaccagtgt tactgcgcgg gctggattga gtcgcgtggc
      781 acaaacggtg cccaaacggc gtcgaacgtg ctgcaatggc ttgctgctgg cttctccatc
      841 ctactgctta tgttttacgc ctaccaaaca tggaagtcaa cctgcggctg ggaggagatc
      901 tatgtgtgcg ctatcgagat ggtcaaggtg attctcgagt tcttcttcga gtttaagaac
      961 ccgtccatgc tgtatctagc cacaggccac cgcgtccagt ggttgcgtta cgccgagtgg
     1021 cttctcacct gcccggtcat ttgcattcac ctgtcaaacc tgacgggctt gtccaacgac
     1081 tacagcaggc gcaccatggg tctgcttgtg tctgatattg gcacaattgt gtggggcgcc
     1141 acttccgcca tggccaccgg atacgtcaag gtcatcttct tctgcctggg tctgtgttat
     1201 ggtgctaaca cgttctttca cgctgccaag gcctacatcg agggttacca caccgtgccg
     1261 aagggccggt gtcgccaggt ggtgactggc atggcttggc tcttcttcgt atcatggggt
     1321 atgttcccca tcctgttcat cctcggcccc gagggcttcg gcgtcctgag cgtgtacggc
     1381 tccaccgtcg gccacaccat cattgacctg atgtcgaaga actgctgggg tctgctcggc
     1441 cactacctgc gcgtgctgat ccacgagcat atcctcatcc acggcgacat tcgcaagacc
     1501 accaaattga acattggtgg cactgagatt gaggtcgaga cgctggtgga ggacgaggcc
     1561 gaggctggcg cggtacccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1741 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1801 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2041 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2161 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2281 gacgagctgt acaagtgata agaattcgat atcaagctta tcgataatca acctctggat
     2341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2701 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc
     2761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2881 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga
     2941 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     3001 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     3061 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     3121 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     3181 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     3241 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     3301 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     3361 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     3421 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     3481 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     3541 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     3601 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     3661 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3721 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
     3781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     3901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     4021 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     4081 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4141 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4201 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4261 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     4321 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4381 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4441 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4501 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4561 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4621 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4681 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4741 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4801 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4861 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4921 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4981 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5041 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5101 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5161 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5221 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5281 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5341 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5401 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5461 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6181 tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc
     6241 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
     6301 agtggccaac tccatcacta ggggttcctg cggccgcacg cgtgttgcgc ctcgtgcaat
     6361 aaataaagat ccattgcctc gccgaaacga taaataaaga tccattccac tgatcgggat
     6421 ataaataaag atccattact gggcctgagg tataaataaa gatccatagt tctctcaacc
     6481 ctataaataa agatccatta ctagaagtat gcaataaata aagatccatt agatattacg
     6541 t
//
