LOCUS       sequence-25676-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST TNRC6A
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      131..140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             140..163
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    206..230
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgagc tcggtcgatc catcgatagt gtggtggaat
      121 tctgatcgcc gccgccatgg actacaagga cgacgatgac aagtaccctt atgacgtgcc
      181 cgattacgct ttgaaccaga tatcaacaag tttgtacaaa aaagcaggct ccaccatggg
      241 aaccatggat gctgattctg cctccagttc tgaatcagag agaaacatca ctatcatggc
      301 ttcagggaac acaggtggtg aaaaagatgg ccttcggaat agcactggac ttggttccca
      361 aaacaagttt gtagttggta gcagcagcaa taatgtgggc catggaagta gtactgggcc
      421 atggggtttt tcccatggag ccataataag cacatgtcag gtctctgtgg atgctcctga
      481 aagcaaatct gaaagtagca acaatagaat gaatgcttgg ggcactgtaa gttcttcatc
      541 aaatggaggg ttaaatccaa gcactttgaa ttcagctagc aaccatggtg cctggccagt
      601 attagagaac aatggacttg ccctaaanng g
//
