LOCUS       sequence-25677-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST TNRC6B
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             179..202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             203..229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     gap             590..606
                     /estimated_length=17
ORIGIN
        1 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccatgga
      181 ctacaaggac gacgatgaca agtaccctta tgacgtgccc gattacgctt tgaaccagat
      241 atcaacaagt ttgtacaaaa aagcaggctc caccatggga accaattcag tcgacatgag
      301 agagaaggag caagaaaggg aagaacagtt aatggaagac aagaaaagga agaaagagga
      361 taaaaagaaa aaagaagcca ctcagaaggt cacggaacaa aaaaccaaag tgcccgaagt
      421 gacgaaacca agtttaagcc aaccaacggc cgccagccca attggcagct ctccatcgcc
      481 accagtcaat ggtggcaaca atgccaaaag ggtggcagtg ccgaacggac aaccgccaag
      541 cgccgcccgc tacatgcctc gggaggtgcc gccgcgattc cgttgccngn nnnnnnnnnn
      601 nnnnnnacna annnnngggc agcccccctc caccgtcctg catgctcctt gggggtgggn
      661 nggctctcct nnnnnnncnc ncnnnnnngn annaanccna aannacgnan annnganngg
      721 nnnnnntgct gcagantgag agtgggactg cgc
//
