LOCUS       sequence-256782-        6551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: pAAV2-ProC3-Cre/mCherry
FEATURES             Location/Qualifiers
     source          1..6551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..1091
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1101..1154
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1155..1862
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1297..1315)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1565..1584
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1890..2478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1943..1963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2361..2372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2510..2986
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2709..2728)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3026..3166
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3026..3155
                     /label=AAV2 ITR
     rep_origin      3241..3696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3328..3347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3538..3559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3713..3732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3832..3854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3892..3910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3978..4082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4083..4151,4152..4943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4301..4320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5114..5702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5603..5622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5764..5893
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        join(6405..6551,1..50)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      join(6452..6551,1..36)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ggatccgcca
       61 ccatggccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
      121 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
      181 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
      241 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
      301 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
      361 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgaa
      421 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
      481 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta
      541 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
      601 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
      661 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
      721 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
      781 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
      841 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
      901 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
      961 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
     1021 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
     1081 aagatggcga tggaccggtc gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     1141 agaatcctgg cccagtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     1201 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     1261 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1321 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     1381 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     1441 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     1501 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     1561 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     1621 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     1681 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     1741 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     1801 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     1861 aatgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     1921 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1981 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2041 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2101 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2161 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2221 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2281 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga
     2341 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2401 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2461 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc
     2521 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     2581 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     2641 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     2701 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     2761 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     2821 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     2881 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     2941 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt
     3001 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     3061 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3121 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     3181 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     3241 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3301 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3361 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3421 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     3481 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3541 gactcttgtt ccaaactgga acaacactca actctatttc gggctattct tttgatttat
     3601 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     3661 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     3721 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5761 tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc
     5821 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     5881 actaggggtt cctgcggccg cacgcgtaaa gctggaactg gggccggaaa cagctgaggg
     5941 tgcccagccg gaaactcgaa atcaacgtag gccggaaact attcgatgaa ttccgccgga
     6001 aactaggtcc ggaggactgc cggaaacacc tgtaatcaag ccgccggaaa cctgttgtgg
     6061 ccgtatgccg gaaacgtctt aattggacgt gccggaaact cttttaatga gttcgccgga
     6121 aaccagacca gccgagctgc cggaaaccgg ttatatagaa cggccggaaa cggtccacag
     6181 gaaaaagccg gaaacaccca aacggttagc gccggaaacg actggggagg acgtgccgga
     6241 aacgtactat ctgaagatgc cggaaacact tgaaagctcc aagccggaaa cgggcccgtg
     6301 cggatagccg gaaactgacg gtacacggcc gccggaaaca ctacttgtat ggtagccgga
     6361 aacttgggcg tggctggggc cggaaacgct cgagatctgc gatctgcatc tcaattagtc
     6421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6481 ccattctccg ccccatcgct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6541 ggcctctgag c
//
