LOCUS       sequence-256783-        6062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: pAAV-ProD1-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        462..493
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      565..574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(571..579,580..597,598..600,601..633,634..636,637..660,
                     661..687,688..744,745..750,751..1020,1021..1038,1039..1473,
                     1474..1479,1480..1923)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1084..1105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1345..1364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1960..2548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2013..2033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2431..2442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2580..3056
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2779..2798)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3096..3236
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3096..3225
                     /label=AAV2 ITR
     rep_origin      3311..3766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3398..3417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3608..3629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3783..3802)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3902..3924
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3962..3980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4048..4152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4153..4221,4222..5013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4371..4390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5184..5772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5673..5692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5834..5963
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 aatcagatgg ttcattgtaa gccactgtag tcgtgggtga ctcacatcaa accacccctt
       61 cgggaacacg atgccgacac tgaaactaca taggggagag caaataaaac tgtcttctct
      121 agtttgggga aattggagcc tgccttaggg aaacagtgac taatttacag ctagtgtttg
      181 gaatgagatc atctgtcaaa ataaatgtat ctttacttcc ttcttgagag gaagcaagct
      241 gttttatgat gttccttgga atccttaaaa atacagagca acatttacat tattaatgat
      301 acgctttatt gctgcaggct aactaggtcc aaaattgtcc ttccatagat ggagctggag
      361 agttacacag aagtttgcat atcgagctct taggtctgca tgtacagcta atgtacttgt
      421 ggaccctgtc acatatcctc acatggtcct gctggagtta gtagagggta tataatggaa
      481 gctcgacttc cagctatcac atccactgtg ttgttgtgaa ctggaatcca ctataggcca
      541 ggatcctgta caccgcgggc tagcgccacc atgggttctc atcatcatca tcatcatggt
      601 atggccagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      661 gatctcgcca ccatggtgga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
      721 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
      781 aacggcatca aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc
      841 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      901 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
      961 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtataag
     1021 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1081 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     1141 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1201 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1261 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     1321 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1381 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1441 ggcaacatcc tggggcacaa gctggagtac aacctgccgg accaactgac tgaagagcag
     1501 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
     1561 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
     1621 caggacatga tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc
     1681 ctgacaatga tggcaagaaa aatgaaatac agggacacgg aagaagaaat tagagaagcg
     1741 ttcggtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
     1801 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
     1861 gacatcgacg gggatggtca ggtaaactac gaagagtttg tgcaaatgat gacagcgaag
     1921 tgagtcgaca gatctgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     1981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2341 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     2401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2461 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2521 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
     2581 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     2641 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     2701 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     2761 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2821 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2881 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2941 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     3001 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     3061 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3121 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3181 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3241 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3301 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3361 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     3421 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3481 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3541 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3601 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     3661 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     3721 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3781 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3841 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3901 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3961 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4021 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5821 tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg
     5881 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     5941 caactccatc actaggggtt cctgcggccg cacgcgtatc ctggggttta aacaggacta
     6001 ggtccactgg gagaagagga agtcaaacag gactagtcat cgataggcta gccagcttaa
     6061 tt
//
