LOCUS       sequence-256787-        7437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: pAAV-ProA1-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..7437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2169..2178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2175..2183,2184..2201,2202..2204,2205..2237,2238..2240,
                     2241..2264,2265..2291,2292..2348,2349..2354,2355..2624,
                     2625..2642,2643..3077,3078..3083,3084..3527)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      2271..2280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2561..2582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2688..2709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2949..2968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3564..4152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3617..3637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4035..4046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4184..4660
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4383..4402)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4700..4840
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4700..4829
                     /label=AAV2 ITR
     rep_origin      4915..5370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5002..5021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5212..5233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5387..5406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5506..5528
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5566..5584)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5652..5756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5757..5825,5826..6617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5975..5994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6788..7376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7277..7296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtagaggc aggccgagtt tgaggccagc ctggtctaca
      181 caggcgttct aggagaacct gtctcatatg cacatgggcc tgggattaca catacaactg
      241 caatggcagc acttggaaag cggaagcagg agaatcggaa tttcacactc atccttcatt
      301 atagaagtcc aaacctgtgc taagctactt ggacatcgct aaaaaacagc aaaaatcttt
      361 ctaggaattg ccaatgtata cacaccaagt tgtatttttg tagggcagac ttttccaact
      421 tgccagatga taataatcat ttatattagc tacatttcag gctccagaat taacactgtt
      481 actgaaatgt ataggttcta aaacatacca tttccaaata ttttaaagga ttaacatttt
      541 tgaaaagcat gtgatcttcc aaccatattt tagggaacag acataagaag taggcaagtt
      601 tgggaagaaa cagttcctga gggcatttct tcccaacctt gaatgccctg tgggttaact
      661 gaggtcttgg taaaatgtag attatttact ggactttttg aactgagagt gcatttctaa
      721 caaaactcca gggtatgtgg tccttgcatt acatatgggg tagcaacatt ctaaagcagt
      781 gtttttcaac ctgtggatca agacccacag aagtcacata gcagatatcc tgcctattag
      841 atatttacat taagattcag agcagtagca gaataggagt catcccaacc tgaggaactg
      901 catcagagtc ccagcatcag gaagggtgag agccactgag ctaaagtcct ctaggtgagg
      961 gggctgcccc ggaaagactc atttaaatga aaccactgac acagagagct gacagatgag
     1021 gtgggttccg tgtctgtgag gctctgctgg tcgtctcctc actcccatgg aagaaccacc
     1081 gagatgaggg cgaggggcag agctaaccca gcctctaagt aggcagagtc tagtgtccag
     1141 ctgcccaagg aagaagtttg ctgtgtgagg tggccctgat gtccgcacac acaaaatgcc
     1201 agtgaagtct acttgaccaa gtgaagctgg tgtggaatgg gaagaagcac acacagtcag
     1261 tctctctgca cacactctgt ccttcacttc ttcacttacc agagatttga tgagaaccta
     1321 ctagcagatc agatttgatc cctgagtgga aaaaacgtac agtgggagat aaaagaggaa
     1381 aacaacggat ctgagtttga ggttagccta gtctgagcaa gccggataca cagtaagacc
     1441 ctgtctcaca tacatccact cgcacgcgcg cgcacacaaa cacacacaca cacacacaca
     1501 cacacacaca cacacacaca catcgtgcta aggacaagat aggcatcctg agagatgagc
     1561 caggacaaag aaccagtaat agctcctgga gcagcacatc tgttttgcca ggattatccc
     1621 ttggatctct taaaaccgag accttgtaat ctgaagactc aacttgggct gtacccttaa
     1681 ccttcagctc tatgatgcaa gtgagtccac aggaccggag gctttgagat gagcttttca
     1741 gaagggagga gttggccgct tgctcccaga gctccagcac ctgcattctt ctggctatgt
     1801 cagaagccag atcatttccc tcgttaaaaa caaaaacaaa aaaacaaaca aacaaaatgt
     1861 tagtctttgc cctttatctg cctggcaaag cttttaattg gcttgatctg tcattccgct
     1921 agacataaag gggacaatcc ccggattagg aaggagctct ccagctcggg taaggagtct
     1981 caaggcaagg taggcaagca ccaccggtcc gcactctcgc ccagctttta cgggaagaag
     2041 agaatgttac tctatcctaa catatttttc cttttcctct atctcacaga taggaaaaat
     2101 ttaagagcca gagggaacgt cccttctcag aggagacagc agaaggatcc tgtacaccgc
     2161 gggctagcgc caccatgggt tctcatcatc atcatcatca tggtatggcc agcatgactg
     2221 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatctc gccaccatgg
     2281 tggactcatc acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc
     2341 tgagctcact cgagaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
     2401 acttccacat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
     2461 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc
     2521 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2581 tgaccgccgc cgggatcact ctcggcatgg acgagctgta taagggcggt accggaggga
     2641 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2701 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
     2761 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2821 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2881 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
     2941 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3001 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3061 acaagctgga gtacaacctg ccggaccaac tgactgaaga gcagatcgca gaatttaaag
     3121 aggctttctc cctatttgac aaggacgggg atgggacaat aacaaccaag gagctgggga
     3181 cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
     3241 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
     3301 gaaaaatgaa atacagggac acggaagaag aaattagaga agcgttcggt gtgtttgata
     3361 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
     3421 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gacggggatg
     3481 gtcaggtaaa ctacgaagag tttgtgcaaa tgatgacagc gaagtgagtc gacagatctg
     3541 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     3601 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3661 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3721 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3781 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3841 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3901 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3961 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     4021 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4081 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4141 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     4201 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     4261 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     4321 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     4381 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     4441 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     4501 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     4561 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     4621 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
     4681 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4741 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4801 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4861 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4921 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4981 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5041 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5101 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     5161 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5221 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     5281 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5341 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     5401 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5461 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5521 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5581 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5641 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5701 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5761 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5821 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5881 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5941 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6001 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6061 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6121 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6181 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6241 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6301 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6361 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6421 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6481 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6541 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6601 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6661 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
