LOCUS       sequence-256788-        6722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 216_pAAV-ProD24-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        456..487
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        1081..1112
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1166..1175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1172..2098
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2102..2104,2105..2107,2108..2821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2102..2104,2105..2107,2108..2818)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2147..2168)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2408..2427)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2849..3437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2902..2922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3320..3331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3469..3945
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3668..3687)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3985..4125
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3985..4114
                     /label=AAV2 ITR
     rep_origin      4200..4655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4287..4306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4497..4518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4672..4691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4791..4813
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4851..4869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4937..5041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5042..5110,5111..5902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5260..5279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6073..6661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6562..6581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatatt
      241 ccggtcacac ggccaagatt attccacctg cgctttgagc aatagggaga gggctctggt
      301 gcctcttcct ggaatttgat taattcgctt gagtcagtca cagaatttga ggaagcattg
      361 atatttgaag atgtgttctt ctaaaggata caaatgaata tatgcatagt gagagtttag
      421 gagatagcat cctcacatgg tcctgctgga gttagtagag ggtatataat ggaagctcga
      481 cttccagcta tcacatccac tgtgttgttg tgaactggaa tccactatag gccaatcctg
      541 gggtttaaac aggactaggt ccactgggag aagaggaagt caaacaggac tagtcatcga
      601 taggctagcc agcttaatta atcagatggt tcattgtaag ccactgtagt cgtgggtgac
      661 tcacatcaaa ccaccccttc gggaacacga tgccgacact gaaactacat aggggagagc
      721 aaataaaact gtcttctcta gtttggggaa attggagcct gccttaggga aacagtgact
      781 aatttacagc tagtgtttgg aatgagatca tctgtcaaaa taaatgtatc tttacttcct
      841 tcttgagagg aagcaagctg ttttatgatg ttccttggaa tccttaaaaa tacagagcaa
      901 catttacatt attaatgata cgctttattg ctgcaggcta actaggtcca aaattgtcct
      961 tccatagatg gagctggaga gttacacaga agtttgcata tcgagctctt aggtctgcat
     1021 gtacagctaa tgtacttgtg gaccctgtca catatcctca catggtcctg ctggagttag
     1081 tagagggtat ataatggaag ctcgacttcc agctatcaca tccactgtgt tgttgtgaac
     1141 tggaatccac tataggccag gatccgccac catggattat ggaggcgccc tgagtgccgt
     1201 tgggcgcgag ctgctatttg taacgaaccc agtagtcgtc aatggctctg tacttgtgcc
     1261 tgaggaccag tgttactgcg cgggctggat tgagtcgcgt ggcacaaacg gtgcccaaac
     1321 ggcgtcgaac gtgctgcaat ggcttgctgc tggcttctcc atcctactgc ttatgtttta
     1381 cgcctaccaa acatggaagt caacctgcgg ctgggaggag atctatgtgt gcgctatcga
     1441 gatggtcaag gtgattctcg agttcttctt cgagtttaag aacccgtcca tgctgtatct
     1501 agccacaggc caccgcgtcc agtggttgcg ttacgccgag tggcttctca cctgcccggt
     1561 catttgcatt cacctgtcaa acctgacggg cttgtccaac gactacagca ggcgcaccat
     1621 gggtctgctt gtgtctgata ttggcacaat tgtgtggggc gccacttccg ccatggccac
     1681 cggatacgtc aaggtcatct tcttctgcct gggtctgtgt tatggtgcta acacgttctt
     1741 tcacgctgcc aaggcctaca tcgagggtta ccacaccgtg ccgaagggcc ggtgtcgcca
     1801 ggtggtgact ggcatggctt ggctcttctt cgtatcatgg ggtatgttcc ccatcctgtt
     1861 catcctcggc cccgagggct tcggcgtcct gagcgtgtac ggctccaccg tcggccacac
     1921 catcattgac ctgatgtcga agaactgctg gggtctgctc ggccactacc tgcgcgtgct
     1981 gatccacgag catatcctca tccacggcga cattcgcaag accaccaaat tgaacattgg
     2041 tggcactgag attgaggtcg agacgctggt ggaggacgag gccgaggctg gcgcggtacc
     2101 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2161 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2221 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2281 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2341 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2401 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2461 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2521 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2581 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2641 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2701 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2761 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg
     2821 ataagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2881 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3241 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     3301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3421 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3481 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3541 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3601 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3661 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3721 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3781 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3841 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3901 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3961 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4021 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4081 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4141 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4261 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4381 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4501 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4561 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4621 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4681 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4741 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4801 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4861 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6721 gt
//
