LOCUS       sequence-256789-        6523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 214_pAAV-ProD22-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        752..783
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             843..1769
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1773..1775,1776..1778,1779..2492)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1773..1775,1776..1778,1779..2489)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1818..1839)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2079..2098)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2520..3108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2573..2593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2991..3002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3140..3616
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3339..3358)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3656..3796
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3656..3785
                     /label=AAV2 ITR
     rep_origin      3871..4326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3958..3977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4168..4189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4343..4362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4462..4484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4522..4540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4608..4712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4713..4781,4782..5573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4931..4950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5744..6332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6233..6252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6394..6523
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag aagaggaagt
       61 caaacaggac tagtcatcga taggctagcc agcttaatta atcagatatt ccggtcacac
      121 ggccaagatt attccacctg cgctttgagc aatagggaga gggctctggt gcctcttcct
      181 ggaatttgat taattcgctt gagtcagtca cagaatttga ggaagcattg atatttgaag
      241 atgtgttctt ctaaaggata caaatgaata tatgcatagt gagagtttag gagatagggg
      301 ttcattgtaa gccactgtag tcgtgggtga ctcacatcaa accacccctt cgggaacacg
      361 atgccgacac tgaaactaca taggggagag caaataaaac tgtcttctct agtttgggga
      421 aattggagcc tgccttaggg aaacagtgac taatttacag ctagtgtttg gaatgagatc
      481 atctgtcaaa ataaatgtat ctttacttcc ttcttgagag gaagcaagct gttttatgat
      541 gttccttgga atccttaaaa atacagagca acatttacat tattaatgat acgctttatt
      601 gctgcaggct aactaggtcc aaaattgtcc ttccatagat ggagctggag agttacacag
      661 aagtttgcat atcgagctct taggtctgca tgtacagcta atgtacttgt ggaccctgtc
      721 acatatcctc acatggtcct gctggagtta gtagagggta tataatggaa gctcgacttc
      781 cagctatcac atccactgtg ttgttgtgaa ctggaatcca ctataggcca ggatccgcca
      841 ccatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
      901 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
      961 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
     1021 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
     1081 gctgggagga gatctatgtg tgcgctatcg agatggtcaa ggtgattctc gagttcttct
     1141 tcgagtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
     1201 gttacgccga gtggcttctc acctgcccgg tcatttgcat tcacctgtca aacctgacgg
     1261 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctgat attggcacaa
     1321 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     1381 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     1441 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     1501 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     1561 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     1621 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     1681 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     1741 tggaggacga ggccgaggct ggcgcggtac ccatggtgag caagggcgag gagctgttca
     1801 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1861 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1921 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1981 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2041 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2101 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2161 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2221 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2281 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2341 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2401 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2461 tcactctcgg catggacgag ctgtacaagt gataagaatt cgatatcaag cttatcgata
     2521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2881 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2941 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     3121 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     3181 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     3241 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3301 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3361 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3421 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3481 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3541 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3601 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     3661 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3721 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3781 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3841 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3901 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     3961 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4021 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4081 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4141 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4201 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4261 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4321 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4381 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4441 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4501 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4561 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4681 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4741 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4801 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4861 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4921 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4981 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5041 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5101 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5161 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5221 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5281 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5341 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5401 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5461 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5521 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5581 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5641 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6361 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
     6421 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     6481 gcgcgcagag agggagtggc caactccatc actaggggtt cct
//
