LOCUS       sequence-25679-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST IGF2BP1
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     68..92
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             192..215
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             216..242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    258..282
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggggcttt agcgactctc cctatcagtg atagagatct ccctatcagt gatagagatc
       61 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      121 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      181 ttggtaccat ggactacaag gacgacgatg acaagtaccc ttatgacgtg cccgattacg
      241 ctttgaacca gatatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      301 cagtcgacat gaacaagctt tacatcggca acctcaacga gagcgtgacc cccgcggact
      361 tggagaaagt gtttgcggag cacaagatct cctacagcgg ccagttcttg gtcaaatccg
      421 gctacgcctt cgtggactgc ccggacgagc actgggcgat gaaggccatc gaaactttct
      481 ccgggaaagt agaattacaa ggaaaacgct tagagattga acattcggtg cccaaaaaaa
      541 caaaggagcc ggaaaattca aatccgaaat attccacccc agctccgatg ggaagtactg
      601 gacagcctgc tggctcagta tggtacagta gagaactgtg agcaagtgaa caccgagagt
      661 nagagncggt gntgaatgtc acctattcca gccgggagca gaccaggcaa gccatcatga
      721 agctgaatgg ccaccagttg gagancatgc ctgangtctg tacatcccga tgagcaatag
      781 cacaggactg agatggcgcg agggcttggc ttcggncncc c
//
