LOCUS       sequence-256793-        6310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 211_pAAV-ProD20-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        669..700
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             760..1686
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1690..1692,1693..1695,1696..2409)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1690..1692,1693..1695,1696..2406)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1735..1756)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1996..2015)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2437..3025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2490..2510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2908..2919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3057..3533
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3256..3275)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3573..3713
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3573..3702
                     /label=AAV2 ITR
     rep_origin      3788..4243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3875..3894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4085..4106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4260..4279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4379..4401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4439..4457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4525..4629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4630..4698,4699..5490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4848..4867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5661..6249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6150..6169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatagg
      241 ggattgaaga gaacagagcc caggaactgg actggagaca gagaaggtaa caaaatggta
      301 tttgtgggtg tttgagagaa gagcagtcgc tcctatccta cactggcttt ggcagcggca
      361 agcagccctc agatctcagc tgagctgaga ggatgctctg aaaccagctt ggccagagcc
      421 ctgaggcagg caggcagatg gatagtcact tcccgacatg tgcaggagcg ataggcagag
      481 tgcagagtcc agggcataga ggcccagaac tactgtctgc tgctccactg agccagagct
      541 ttgctgacca agtgttctga catggctgta ttatgccctg ttgtctgggc actaaatttt
      601 atacctcctg agattccata tggcttgggt tggtgcagca aatcctcaca tggtcctgct
      661 ggagttagta gagggtatat aatggaagct cgacttccag ctatcacatc cactgtgttg
      721 ttgtgaactg gaatccacta taggccagga tccgccacca tggattatgg aggcgccctg
      781 agtgccgttg ggcgcgagct gctatttgta acgaacccag tagtcgtcaa tggctctgta
      841 cttgtgcctg aggaccagtg ttactgcgcg ggctggattg agtcgcgtgg cacaaacggt
      901 gcccaaacgg cgtcgaacgt gctgcaatgg cttgctgctg gcttctccat cctactgctt
      961 atgttttacg cctaccaaac atggaagtca acctgcggct gggaggagat ctatgtgtgc
     1021 gctatcgaga tggtcaaggt gattctcgag ttcttcttcg agtttaagaa cccgtccatg
     1081 ctgtatctag ccacaggcca ccgcgtccag tggttgcgtt acgccgagtg gcttctcacc
     1141 tgcccggtca tttgcattca cctgtcaaac ctgacgggct tgtccaacga ctacagcagg
     1201 cgcaccatgg gtctgcttgt gtctgatatt ggcacaattg tgtggggcgc cacttccgcc
     1261 atggccaccg gatacgtcaa ggtcatcttc ttctgcctgg gtctgtgtta tggtgctaac
     1321 acgttctttc acgctgccaa ggcctacatc gagggttacc acaccgtgcc gaagggccgg
     1381 tgtcgccagg tggtgactgg catggcttgg ctcttcttcg tatcatgggg tatgttcccc
     1441 atcctgttca tcctcggccc cgagggcttc ggcgtcctga gcgtgtacgg ctccaccgtc
     1501 ggccacacca tcattgacct gatgtcgaag aactgctggg gtctgctcgg ccactacctg
     1561 cgcgtgctga tccacgagca tatcctcatc cacggcgaca ttcgcaagac caccaaattg
     1621 aacattggtg gcactgagat tgaggtcgag acgctggtgg aggacgaggc cgaggctggc
     1681 gcggtaccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1741 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1801 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1861 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1921 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1981 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2041 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2101 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2161 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2221 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2281 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2341 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2401 tacaagtgat aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     2461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2581 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2641 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2701 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2761 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2821 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     2881 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2941 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3001 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     3061 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     3121 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     3181 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     3241 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     3301 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     3361 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     3421 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     3481 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     3541 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     3601 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3661 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     3721 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     3781 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3841 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     3901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4081 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     4141 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     4201 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     4261 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4321 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4381 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4441 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6301 gctcacatgt
//
