LOCUS       sequence-256811-       10595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Borsos M, Perricone SM, Schauer T, Pontabry J, de Luca KL, de Vries 
            SS, Ruiz-Morales ER, Torres-Padilla ME, Kind J
  TITLE     Genome-lamina interactions are established de novo in the early 
            mouse embryo.
  JOURNAL   Nature. 2019 May;569(7758):729-733. doi: 10.1038/s41586-019-1233-0. 
            Epub 2019 May 22.
  PUBMED    31118510
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1233-0"; journalName: "Nature"; date: "2019-05";
            volume: "569"; issue: "7758"; pages: "729-733"
COMMENT     Sequence Label: Lenti_AID-Dam-lamin B1
FEATURES             Location/Qualifiers
     source          1..10595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..46
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    163..184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(199..204,205..222,223..229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    237..253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     242..264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     261..277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     261..277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     296..316
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        298..316
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        417..796
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        797..1000
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     951..971
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1015..1195
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1242..1367
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1860..2093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2278..2322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2587..2704
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2750..3260
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3179..3197
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3264..3273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3276..3302
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3312..3992
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             4845..4886
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4902..4926
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6730..6754)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6782..7370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6835..6855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7253..7264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7457..7690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7762..7883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7799..7818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7853..7872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7923..8058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8078..8097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8079..8097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8107..8124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8107..8123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8116..8138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8265..8720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8352..8371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8562..8583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8746..8850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8851..8919,8920..9711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9069..9088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9882..10470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10371..10390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
       61 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
      301 taaccctcac taaagggaac aaaagctgga gctgcaagct tggccattgc atacgttgta
      361 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca
      421 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      481 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      541 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      601 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      661 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      721 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      781 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      841 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      901 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      961 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accggggtct
     1021 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1081 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1141 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1201 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1261 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1321 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1381 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1441 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1501 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1561 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1621 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1681 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1741 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1801 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1861 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1921 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1981 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     2041 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     2101 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     2161 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2221 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2281 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2341 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2401 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2461 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2521 gaaggtggag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2581 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2641 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2701 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattccacg gggttggggt
     2761 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2821 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     2881 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     2941 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3001 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     3061 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3121 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3181 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3241 tcaccgacct ctctccccag ggggccgcca tgggctaccc atacgatgtt cctgactatg
     3301 cgggatccaa tggcagtgtc gagctgaatc tgagggagac tgagctgtgt cttggtcttc
     3361 ccggtggaga tacagtggct ccggtaaccg gaaacaagag agggttctca gagacggttg
     3421 atctgaagct aaatctgaat aatgagcctg caaacaagga aggatctacg actcatgacg
     3481 tcgtgacttt tgattccaag gagaagagtg cttgtcctaa agatccagcc aaacctccgg
     3541 ccaaggcaca agttgtggga tggccaccgg tgagatcata ccggaagaac gtgatggttt
     3601 cctgccaaaa atcaagcggt ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg
     3661 gagcaccgta cttgaggaaa atcgatttga ggatgtataa aagctacgat gagctttcta
     3721 atgctttgtc caacatgttc agctctttta ccatgggcaa acatggagga gaagaaggaa
     3781 tgatagactt catgaatgag aggaaattga tggatttggt gaatagctgg gactatgttc
     3841 cctcttatga agacaaagac ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt
     3901 tcgtcgatac atgcaagcgt ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc
     3961 cgagggcgat ggagaagtgc aagagcagag ctgcggcctc caccatgaag aaaaatcgcg
     4021 cttttttgaa gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa cggcatttgc
     4081 ccaagggcga atgtctggtt gagccttttg taggtgccgg gtcggtgttt ctcaacaccg
     4141 acttttctcg ttatatcctt gccgatatca atagcgacct gatcagtctc tataacattg
     4201 tgaagatgcg tactgatgag tacgtacagg ccgcacgcga gctgtttgtt cccgaaacaa
     4261 attgcgccga ggtttactat cagttccgcg aagagttcaa caaaagccag gatccgttcc
     4321 gtcgggcggt actgttttta tatttgaacc gctacggtta caacggcctg tgtcgttaca
     4381 atctgcgcgg tgagtttaac gtgccgttcg gccgctacaa aaaaccctat ttcccggaag
     4441 cagagttgta tcacttcgct gaaaaagcgc agaatgcctt tttctattgt gagtcttacg
     4501 ccgatagcat ggcgcgcgca gatgatgcat ccgtcgtcta ttgcgatccg ccttatgcac
     4561 cgctgtctgc gaccgccaac tttacggcgt atcacacaaa cagttttacg cttgaacaac
     4621 aagcgcatct ggcggagatc gccgaaggtc tggttgagcg ccatattcca gtgctgatct
     4681 ccaatcacga tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg catgtcgtca
     4741 aagttcgacg cagtataagc agcaacggcg gcacacgtaa aaaggtggac gaactgctgg
     4801 ctttgtacaa accaggagtc gtttcacccg cgaaaaaaac cggtggtaag cctatcccta
     4861 accctctcct cggtctcgat tctacgatcg atttcgatca aacaagtttg tacaaaaaag
     4921 caggctccgc ggccgccccc ttcaccatgg cgaccgcgac ccccgtgcag cagcagcggg
     4981 cgggcagccg cgccagcgcc cccgccacgc cgctcagccc cacgcgcctg tcgcgcctgc
     5041 aggagaaaga ggagctgcgg gagctcaacg accgcctggc tgtgtacatc gataaggtgc
     5101 gcagcctgga gacggagaac agcgcgctgc agctgcaggt gaccgagcgc gaggaggtgc
     5161 gcggccgcga gctcaccggc ctcaaggctc tctacgagac cgagctggcc gacgcacgcc
     5221 gcgctctgga cgacacggcc cgcgagcgcg ccaagctcca gatcgagctg ggcaagttca
     5281 aggccgagca cgaccagctg ctgctcaatt atgccaagaa ggaatctgat ctcagtggag
     5341 cccagatcaa gcttcgagag tatgaggcgg cactaaactc taaggatgcg gcgctggcta
     5401 ctgccctagg ggacaaaaag agtttagagg gagacttgga ggatctgaaa gatcagattg
     5461 cccagctaga agcatcctta tctgccgcca aaaagcagtt agcagatgaa actttactta
     5521 aagtggattt ggagaatcgc tgtcagagcc ttactgagga cttggagttt cgtaaaaata
     5581 tgtatgaaga ggagatcaat gagacaagga ggaagcatga gacccgcttg gtggaagtgg
     5641 actctgggcg tcagattgag tatgagtaca agctggctca agccctgcat gagatgcggg
     5701 agcagcacga cgcgcaggtg aggctgtaca aggaagagct ggagcagacc taccacgcca
     5761 agcttgagaa tgccagactc tcctcagaga tgaacacttc cactgtcaac agtgcccggg
     5821 aagagctgat ggagagccgg atgaggatcg agagcctctc ctcacagctc tctaacctgc
     5881 agaaagagtc tagagcgtgt ttggaaagga tccaggaatt ggaggacatg cttgctaagg
     5941 agagagacaa ctcgcgccgc atgctgtctg acagagagag agagatggcg gagatcaggg
     6001 accagatgca gcagcagctg agtgattatg agcagctgct ggacgtgaag ctggccctgg
     6061 acatggagat cagcgcctac aggaagctcc tggaaggcga agaagagcgg ttaaagctct
     6121 ctccaagccc ttcttcccgg gtgaccgtgt ccagagcgtc ctccagtcgc agtgtgcgca
     6181 ccaccagagg aaagcggaag agagttgatg tggaggagtc ggaggcgagc agcagtgtta
     6241 gcatttccca ctctgcctca gccacgggga acgtgtgcat tgaagagata gatgttgatg
     6301 ggaagtttat tcgcttgaag aacacttctg agcaggatca accaatggga ggctgggaga
     6361 tgatcagaaa aattggagac acatcagtca gttacaaata tacctcaaga tatgtgctga
     6421 aggctggcca gactgtcaca gtgtgggctg caaatgctgg cgtcacagcc agccctccaa
     6481 ctgacctcat ctggaagaac cagaactctt ggggtactgg tgaagatgtg aaggttatat
     6541 tgaagaattc tcagggagag gaggttgctc agagaagcac agtcttcaag accaccatac
     6601 ccgaggagga ggaggaggag gaggaggagc ccatcggagt ggctgtggag gaggagcgtt
     6661 tccaccagca gggagcccca agagcatcca ataaaagctg tgccattatg tgaaagggtg
     6721 ggcgcgccga cccagctttc ttgtacaaag tggttcgatc gaaccgcggt ctagactcga
     6781 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6841 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6901 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6961 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7021 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7081 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7141 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7201 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7261 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7321 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
     7381 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7441 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7501 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7561 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7621 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7681 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7741 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7801 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7861 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7981 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8041 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     8101 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8161 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8221 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     8281 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8341 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8401 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8461 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8521 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8581 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8641 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8701 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8821 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8881 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8941 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9001 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9061 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9121 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9181 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9241 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9301 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9361 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9421 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9481 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9541 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9601 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9661 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9721 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10021 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10081 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10141 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10201 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10261 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10321 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10381 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10441 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10501 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10561 gataaccgta ttaccgcctt tgagtgagct gatac
//
