LOCUS       sequence-256828-        5783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of secretory immunoglobulin heavy chains in mammalian 
            cells, mouse (C57BL/6), IgG2b isotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Lorenzo C, Delgado P, Busse CE, Sanz-Bravo A, Martos-Folgado I, 
            Bonzon-Kulichenko E, Ferrarini A, Gonzalez-Valdes IB, Mur SM, 
            Roldan-Montero R, Martinez-Lopez D, Martin-Ventura JL, Vazquez J, 
            Wardemann H, Ramiro AR
  TITLE     ALDH4A1 is an atherosclerosis auto-antigen targeted by protective 
            antibodies.
  JOURNAL   Nature. 2021 Jan;589(7841):287-292. doi: 10.1038/s41586-020-2993-2. 
            Epub 2020 Dec 2.
  PUBMED    33268892
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2993-2"; journalName: "Nature"; date: "2021-01";
            volume: "589"; issue: "7841"; pages: "287-292"
COMMENT     Sequence Label: AbVec2.0-mIghg2b
FEATURES             Location/Qualifiers
     source          1..5783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     829..853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1094..1113
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2318..2327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2331..2465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2368..2387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2422..2441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2534..2891
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2534..2725
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2557..2577)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2742..2877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2911..2928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2911..2927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2920..2942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3140..3595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3227..3246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3437..3458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3612..3631)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3731..3753
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3791..3809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3877..3981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3982..4050,4051..4842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4200..4219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5013..5601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5502..5521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5755..5772
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
      241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
     1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
     1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
     1201 ctgcaaccgg ttgactaact agctgtgatc aaaacaacac ccccaagcgt ctatccactg
     1261 gcccctgggt gtggagatac aactggttcc tctgtgactc tgggatgcct ggtcaagggc
     1321 tacttccctg agtcagtgac tgtgacttgg aactctggat ccctgtccag cagtgtgcac
     1381 accttcccag ctctcctgca gtctggactc tacactatga gcagctcagt gactgtcccc
     1441 tccagcacct ggccaagtca gaccgtcacc tgcagcgttg ctcacccagc cagcagcacc
     1501 acggtggaca aaaaacttga gcccagcggg cccatttcaa caatcaaccc ctgtcctcca
     1561 tgcaaggagt gtcacaaatg cccagctcct aacctcgagg gtggaccatc cgtcttcatc
     1621 ttccctccaa atatcaagga tgtactcatg atctccctga cacccaaggt cacgtgtgtg
     1681 gtggtggatg tgagcgagga tgacccagac gtccggatca gctggtttgt gaacaacgtg
     1741 gaagtacaca cagctcagac acaaacccat agagaggatt acaacagtac tatccgggtg
     1801 gtcagtgccc tccccatcca gcaccaggac tggatgagtg gcaaggagtt caaatgcaag
     1861 gtcaacaaca aagacctccc atcacccatc gagagaacca tctcaaaaat taaagggcta
     1921 gtcagagctc cacaagtata catcttgccg ccaccagcag agcagttgtc caggaaagat
     1981 gtcagtctca cttgcctggt cgtgggcttc aaccctggag acatcagtgt ggagtggacc
     2041 agcaatgggc atacagagga gaactacaag gacaccgcac cagtcctgga ctctgacggt
     2101 tcttacttca tatacagcaa gctcgatata aaaacaagca agtgggagaa aacagattcc
     2161 ttctcatgca acgtgagaca cgagggtctg aaaaattact acctgaagaa gaccatctcc
     2221 cggtctccgg gtaaatgagc tcagcaccca caaagctctc aggtcctaag agacactggc
     2281 acccatatcc atgcatccct tgtataaata aagcttggcc gccatggccc aacttgttta
     2341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2461 ggatcgggaa ttaattcggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     2521 ggttaggtac cttctgaggc ggaaagaacc atctgtggaa tgtgtgtcag ttagggtgtg
     2581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2881 gcttttgcaa aaagctgtta acagcttggc actggccgtc gttttacaac gtcgtgactg
     2941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagttg
     3001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3061 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3121 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     3361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3481 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3541 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     3601 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3661 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3721 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3841 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4201 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4501 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5701 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5761 tcagtgagcg aggaagcgga aga
//
