LOCUS       sequence-25683-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST EIF2C4
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             142..165
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    208..232
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgag ctcggatcga tccatcgata gtgtggtgga
      121 attctgatcg ccgccgccat ggactacaag gacgacgatg acaagtaccc ttatgacgtg
      181 cccgattacg ctttgaacca gatatcaaca agtttgtaca aaaaagcagg ctccaccatg
      241 gaggcgctgg gacccggacc tccggctagc ctgtttcagc cacctcgtcg tcctggcctt
      301 ggaactgttg gaaaaccaat tcgactgtta gccaatcatt ttcaggttca gattcctaaa
      361 atagatgtgt atcactatga tgtggatatt aagcctgaaa aacggcctcg tagagtcaac
      421 agggaggtag tagatacaat ggtgcggcac ttcaagatgc aaatatttgg tgatcggcag
      481 cctgggtatg atggcaaaag aaacatgtac acagcacatc cactaccaat tggacgggat
      541 agggttgata tggaggtgac tcttccaggc gagggtaaag accaaacatt taaagtgtct
      601 gttcagtggg tgtcagttgt gagccttcag ttgnntnnna nannnnnntg nnngggnnnn
      661 nnnnntgaan tcccnnnnan nnantnnnnt acaagccncn ttgatgttat cacaagacac
      721 ctttcccntc catgganggt a
//
