LOCUS       sequence-25685-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Ancrile B, Lim KH, Counter CM
  TITLE     Oncogenic Ras-induced secretion of IL6 is required for 
            tumorigenesis.
  JOURNAL   Genes Dev. 2007 Jul 15. 21(14):1714-9.
  PUBMED    17639077
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Jul 15. 21(14):1714-9."
COMMENT     Sequence Label: pSuperRetropuro-IL-6 shRNA 1
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     41..57
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(191..210)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(391..414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctcnctcct tctctaggcg ccggaattag atcgatctct cgaggtcgac ggtatcgata
       61 agcttaaaaa agatggatgc ttccaatctg gtctcttgaa ccagattgga agcatccatc
      121 tggggatctc tatcactgat agggaactta taagattccc aaatccaaag acatttcacg
      181 tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg ccactcccct
      241 gtccctcaca gccatcttcc tgccagggcg cacgcgcgct gggtgttccc gcctagtgac
      301 actgggcccg cgattccttg gagcgggttg atgacgtcag cgttcgaatt ctaccgggta
      361 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
      421 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
      481 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
      541 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
      601 acta
//
