LOCUS       sequence-256904-        5765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of secretory immunoglobulin heavy chains in mammalian 
            cells, mouse (C57BL/6), IgG3 isotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  TITLE     More mouse immunoglobulin expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec2.0-mIghg3
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     829..853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1094..1113
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2300..2309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2313..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2350..2369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2404..2423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2516..2873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2516..2707
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2539..2559)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2724..2859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2893..2910)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2893..2909)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2902..2924)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3122..3577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3209..3228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3419..3440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3594..3613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3713..3735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3773..3791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3859..3963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3964..4032,4033..4824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4182..4201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4995..5583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5484..5503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5737..5754
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
      241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
     1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
     1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
     1201 ctgcaaccgg ttgactaact agctgtgatc acaacaacag ccccaagcgt ctatcccttg
     1261 gtccctggct gcggtgacac atctggatcc tcggtgacac tgggatgcct tgtcaaaggc
     1321 tacttccctg agccggtaac tgtaaaatgg aactatggag ccctgtccag cggtgtgcgc
     1381 acagtctcat ctgtactgca gtctgggttc tattccctca gcagcttggt gactgtaccc
     1441 tccagcacct ggcccagcca gactgtcatc tgcaacgtag cccacccagc cagcaagact
     1501 gagttgatta agagaatcga gcctagaata cccaagccca gtaccccccc aggttcttca
     1561 tgcccacctg gtaacatctt gggtggacca tccgtcttca tcttcccccc aaagcccaag
     1621 gatgcactca tgatctccct aacccccaag gttacgtgtg tggtggtgga tgtgagcgag
     1681 gatgacccag atgtccatgt cagctggttt gtggacaaca aagaagtaca cacagcctgg
     1741 acgcagcccc gtgaagctca gtacaacagt accttccgag tggtcagtgc cctccccatc
     1801 cagcaccagg actggatgag gggcaaggag ttcaaatgca aggtcaacaa caaagccctc
     1861 ccagccccca tcgagagaac catctcaaaa cccaaaggaa gagcccagac acctcaagta
     1921 tacaccatac ccccacctcg tgaacaaatg tccaagaaga aggttagtct gacctgcctg
     1981 gtcaccaact tcttctctga agccatcagt gtggagtggg aaaggaacgg agaactggag
     2041 caggattaca agaacactcc acccatcctg gactcggatg ggacctactt cctctacagc
     2101 aagctcactg tggatacaga cagttggttg caaggagaaa tttttacctg ctccgtggtg
     2161 catgaggctc tccataacca ccacacacag aagaacctgt ctcgctcccc tggtaaatga
     2221 gaacagcacc tagccattcc tcgggtctta caagacactg ataccagccc taaccgctga
     2281 accctataaa taaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg
     2461 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
     2521 gcggaaagaa ccatctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2581 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2701 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2761 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     2821 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt
     2881 taacagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2941 ccaacttaat cgccttgcag cacatccccc tttcgccagt tggcgtaata gcgaagaggc
     3001 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     3061 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     3121 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3301 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     3361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3421 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     3481 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3541 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     3601 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3661 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3721 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3781 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3961 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4141 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4201 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4501 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4801 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4861 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5101 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5761 gaaga
//
