LOCUS       sequence-256913-        6432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 185_pAAV-ProD16-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        661..692
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      746..755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             752..1678
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1682..1684,1685..1687,1688..2401)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1682..1684,1685..1687,1688..2398)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1727..1748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1988..2007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2429..3017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2482..2502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2900..2911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3049..3525
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3248..3267)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3565..3705
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3565..3694
                     /label=AAV2 ITR
     rep_origin      3780..4235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3867..3886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4077..4098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4252..4271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4371..4393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4431..4449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4517..4621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4622..4690,4691..5482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4840..4859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6142..6161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6303..6432
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag aagaggaagt
       61 caaacaggac tagtcatcga taggctagcc agcttaatta atcagataga gttgaacaag
      121 cagtaccaaa gcgggtagca ttggtgccgc gtgcggtaac tcagtgtaac tcgttcaccg
      181 gcagacttca acattcattt ctgactgatt cagcagtcag agcgccaaac ttccagtaat
      241 ctctctgaag atttggcaca gatacggagt tcagaatagg aacaagtgtg cactgatgga
      301 gaggagctac tacggctatt tctaaaaata gcatggctgc aggcttccgg aggcttagga
      361 ttctcacgaa acgaaactga gagattctta agatagaacc aggagactgt agctgaagat
      421 tcctgactga cgtgaatatg acttccttta tcccaaagga tgcagtgact tctcaggctc
      481 gggtagcatc atgattagtt ataattgact ttgcctttta atttttaagg caatctataa
      541 attatggatt gttgtgtcaa tcggaagaca ggattttgac atcgtacacc atatacctca
      601 tgattgtcgt caaaaccaag tcctttatcg gggatcctca catggtcctg ctggagttag
      661 tagagggtat ataatggaag ctcgacttcc agctatcaca tccactgtgt tgttgtgaac
      721 tggaatccac tataggccag gatccgccac catggattat ggaggcgccc tgagtgccgt
      781 tgggcgcgag ctgctatttg taacgaaccc agtagtcgtc aatggctctg tacttgtgcc
      841 tgaggaccag tgttactgcg cgggctggat tgagtcgcgt ggcacaaacg gtgcccaaac
      901 ggcgtcgaac gtgctgcaat ggcttgctgc tggcttctcc atcctactgc ttatgtttta
      961 cgcctaccaa acatggaagt caacctgcgg ctgggaggag atctatgtgt gcgctatcga
     1021 gatggtcaag gtgattctcg agttcttctt cgagtttaag aacccgtcca tgctgtatct
     1081 agccacaggc caccgcgtcc agtggttgcg ttacgccgag tggcttctca cctgcccggt
     1141 catttgcatt cacctgtcaa acctgacggg cttgtccaac gactacagca ggcgcaccat
     1201 gggtctgctt gtgtctgata ttggcacaat tgtgtggggc gccacttccg ccatggccac
     1261 cggatacgtc aaggtcatct tcttctgcct gggtctgtgt tatggtgcta acacgttctt
     1321 tcacgctgcc aaggcctaca tcgagggtta ccacaccgtg ccgaagggcc ggtgtcgcca
     1381 ggtggtgact ggcatggctt ggctcttctt cgtatcatgg ggtatgttcc ccatcctgtt
     1441 catcctcggc cccgagggct tcggcgtcct gagcgtgtac ggctccaccg tcggccacac
     1501 catcattgac ctgatgtcga agaactgctg gggtctgctc ggccactacc tgcgcgtgct
     1561 gatccacgag catatcctca tccacggcga cattcgcaag accaccaaat tgaacattgg
     1621 tggcactgag attgaggtcg agacgctggt ggaggacgag gccgaggctg gcgcggtacc
     1681 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1801 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2101 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2161 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2221 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2281 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2341 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg
     2401 ataagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2461 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2521 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2581 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2641 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2701 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2761 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2821 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     2881 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2941 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3001 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3061 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3121 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3181 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3241 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3301 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3361 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3421 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3481 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3541 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3601 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3661 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3721 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3781 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3841 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3901 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3961 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4021 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4081 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4141 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4201 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4261 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4321 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4381 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4441 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4501 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4621 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4681 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4741 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4801 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4861 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4921 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4981 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5041 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5101 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5161 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5221 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5281 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5341 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5401 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5461 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5521 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6301 gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct
     6361 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
     6421 ctaggggttc ct
//
