LOCUS       sequence-256921-        6405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 182_pAAV-ProD13-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        634..665
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      719..728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             725..1651
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1655..1657,1658..1660,1661..2374)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1655..1657,1658..1660,1661..2371)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1700..1721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1961..1980)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2402..2990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2455..2475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2873..2884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3022..3498
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3221..3240)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3538..3678
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3538..3667
                     /label=AAV2 ITR
     rep_origin      3753..4208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3840..3859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4050..4071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4225..4244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4344..4366
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4404..4422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4490..4594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4595..4663,4664..5455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4813..4832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5626..6214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6115..6134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6276..6405
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag aagaggaagt
       61 caaacaggac tagtcatcga taggctagcc agcttaatta atcagatgcc attctcctgg
      121 tcccctcttt gaatatctac aaggtttgca aatgattcac aatgcctcaa actcgagtga
      181 tttaatacta tatgaacatt gaaagtgcca agtgtcagag gagacacaaa actgttgttt
      241 cttcacacac tgaaaataac cagaagcctt ctctcatttc ctggacgcga gaaaagacca
      301 cccctgaata ctagtgtggg tacgtgcacc agcgtcccga gtcgactaga gagccaggtc
      361 actggcctgt gcctctatct gctgaactgc accaaaatct ctggggccag ctgaagaacc
      421 agaattttaa tgcactttcc tcccaggtgt ctaatccaag aaaatgcaca caaaatgcca
      481 agctttccca aagcaaggag gatcagagca gcctgagaga aaagcacttt tttttctgat
      541 gagattccaa gtgacacact gtatttccag actcagaatt tctaggcgtg atgtctagaa
      601 cctgacatcc tcacatggtc ctgctggagt tagtagaggg tatataatgg aagctcgact
      661 tccagctatc acatccactg tgttgttgtg aactggaatc cactataggc caggatccgc
      721 caccatggat tatggaggcg ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
      781 cccagtagtc gtcaatggct ctgtacttgt gcctgaggac cagtgttact gcgcgggctg
      841 gattgagtcg cgtggcacaa acggtgccca aacggcgtcg aacgtgctgc aatggcttgc
      901 tgctggcttc tccatcctac tgcttatgtt ttacgcctac caaacatgga agtcaacctg
      961 cggctgggag gagatctatg tgtgcgctat cgagatggtc aaggtgattc tcgagttctt
     1021 cttcgagttt aagaacccgt ccatgctgta tctagccaca ggccaccgcg tccagtggtt
     1081 gcgttacgcc gagtggcttc tcacctgccc ggtcatttgc attcacctgt caaacctgac
     1141 gggcttgtcc aacgactaca gcaggcgcac catgggtctg cttgtgtctg atattggcac
     1201 aattgtgtgg ggcgccactt ccgccatggc caccggatac gtcaaggtca tcttcttctg
     1261 cctgggtctg tgttatggtg ctaacacgtt ctttcacgct gccaaggcct acatcgaggg
     1321 ttaccacacc gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg cttggctctt
     1381 cttcgtatca tggggtatgt tccccatcct gttcatcctc ggccccgagg gcttcggcgt
     1441 cctgagcgtg tacggctcca ccgtcggcca caccatcatt gacctgatgt cgaagaactg
     1501 ctggggtctg ctcggccact acctgcgcgt gctgatccac gagcatatcc tcatccacgg
     1561 cgacattcgc aagaccacca aattgaacat tggtggcact gagattgagg tcgagacgct
     1621 ggtggaggac gaggccgagg ctggcgcggt acccatggtg agcaagggcg aggagctgtt
     1681 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1741 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1801 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1861 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1921 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1981 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2041 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2101 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2161 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2221 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2281 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2341 gatcactctc ggcatggacg agctgtacaa gtgataagaa ttcgatatca agcttatcga
     2401 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2761 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2821 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3001 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3061 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3121 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3181 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3241 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3301 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3361 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3421 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3481 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3541 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3601 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3661 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3721 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3901 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3961 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4021 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4081 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4141 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4201 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4261 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4321 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4381 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4441 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4501 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4561 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4621 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4681 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4741 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4801 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4861 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4921 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4981 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5041 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5101 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5161 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5221 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5281 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5341 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5401 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5461 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5521 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5581 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5641 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5701 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5761 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5821 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5881 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5941 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6001 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6061 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6121 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6181 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6241 gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc gcgctcgctc
     6301 gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     6361 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcct
//
